Combination | Temperature | Cultivar | Variant | Immobile J2 after 24 h | Immobile J2 after 48 h | Immobile J2 immersed in water | Immobile J2 after NaOH treatment |
---|---|---|---|---|---|---|---|
1 | 10°C | Ina | J2+H2O | 0 ± 0 i | 0 ± 0 k | 0 ± 0g | 0 ± 0 f |
2 | 10°C | Ina | J2+S+H2O | 34.72 ± 13.062 de | 29.44 ± 12.936 hi | 2.778 ± 1.297 ef | 2.778 ± 1.297 de |
3 | 10°C | Ina | J2+StS+H2O | 43.33 ± 12.713 c | 30.56 ± 15.031 ghi | 1.111 ±2.171 fg | 1.111 ±2.171 ef |
4 | 10°C | Ina | J2+RD | 0 ± 0 i | 0 ± 0 k | 0±0g | 0 ± 0 f |
5 | 10°C | Ina | J2+S+RD | 8.06 ± 4.597 h | 22.22 ± 13.731 ij | 1.389 ± 1.716 fg | 1.667 ±2.247 ef |
6 | 10°C | Ina | J2+StS+RD | 20 ± 9.535 f | 34.44 ±8.165 fgh | 1.944 ± 1.716 fg | 1.944 ± 1.716 ef |
7 | 10°C | Jaga | J2+H2O | 0 ± 0 i | 0 ± 0 k | 0±0g | 0 ± 0 f |
8 | 10°C | Jaga | J2+S+H2O | 0 ± 0 i | 0 ± 0 k | 0±0g | 0 ± 0 f |
9 | 10°C | Jaga | J2+StS+H2O | 0 ± 0 i | 0 ± 0 k | 0±0g | 0 ± 0 f |
10 | 10°C | Jaga | J2+RD | 0 ± 0 i | 0 ± 0 k | 0±0g | 0 ± 0 f |
11 | 10°C | Jaga | J2+S+RD | 0 ± 0 i | 0 ± 0 k | 0±0g | 0 ± 0 f |
12 | 10°C | Jaga | J2+StS+RD | 0 ± 0 i | 0 ± 0 k | 0±0g | 0 ± 0 f |
13 | 17°C | Ina | J2+H2O | 0 ± 0 i | 0 ± 0 k | 0±0g | 0 ± 0 f |
14 | 17°C | Ina | J2+S+H2O | 18.33 ± 10.2 fg | 14.72 ± 19.667 j | 0±0g | 0 ± 0 f |
15 | 17°C | Ina | J2+StS+H2O | 21.94 ± 15.6 f | 24.72 ± 15.793 i | 4.167 ±4.949 de | 4.167 ± 4.949 d |
16 | 17°C | Ina | J2+RD | 0 ± 0 i | 0 ± 0 k | 0±0g | 0 ± 0 f |
17 | 17°C | Ina | J2+S+RD | 36.39 ± 10.489 d | 48.89 ± 15.33 d | 0±0g | 0 ± 0 f |
18 | 17°C | Ina | J2+StS+ RD | 36.11 ± 9.83 d | 38.89 ± 14.094 efg | 1.667 ±1.741 fg | 1.667 ± 1.741 ef |
19 | 17°C | Jaga | J2+ H2O | 0 ± 0 i | 0 ± 0 k | 0±0g | 0 ± 0 f |
20 | 17°C | Jaga | J2+S+H2O | 0 ± 0 i | 0 ± 0 k | 0±0g | 0 ± 0 f |
21 | 17°C | Jaga | J2+StS+H2O | 0 ± 0 i | 0 ± 0 k | 0±0g | 0 ± 0 f |
22 | 17°C | Jaga | J2+RD | 0 ± 0 i | 0 ± 0 k | 0±0g | 0 ± 0 f |
23 | 17°C | Jaga | J2+S+ RD | 0 ± 0 i | 45.83 ± 14.848 de | 1.667 ±2.247 fg | 1.667 ±2.247 ef |
24 | 17°C | Jaga | J2+StS+RD | 0 ± 0 i | 39.17 ± 14.293 efg | 0.556 ± 1.297 g | 0.556 ± 1.297 f |
25 | 21 °C | Ina | J2+H2O | 0 ± 0 i | 0 ± 0 k | 0±0g | 0 ± 0 f |
26 | 21°C | Ina | J2+S+H20 | 17.78 ± 10.184 fg | 39.72 ± 11.322 ef | 7.778 ±4.103 c | 7.778 ±4.103 c |
27 | 21°C | Ina | J2+StS+H2O | 29.44 ± 9.727 e | 38.33 ± 9.924 efg | 29.167 ± 10.648 a | 29.167 ± 10.648 a |
28 | 21°C | Ina | J2+RD | 0 ± 0 i | 0 ± 0 k | 0±0g | 0 ± 0 f |
29 | 21°C | Ina | J2+S+RD | 53.61 ± 14.387 b | 71.11 ± 22.398 c | 1.389 ± 1.716 fg | 1.389 ± 1.716 ef |
30 | 21°C | Ina | J2+StS+RD | 100 ± 0 a | 100 ± 0 a | 0.556 ± 1.297 g | 0.556 ± 1.297 f |
31 | 21°C | Jaga | J2+H2O | 0 ± 0 i | 0 ± 0 k | 0±0g | 0 ± 0 f |
32 | 21°C | Jaga | J2+S+H2O | 14.44 ± 7.566 g | 44.17 ± 15.707 de | 6.944 ±3.001 cd | 6.944 ± 3.001 c |
33 | 21°C | Jaga | J2+StS+H2O | 16.67 ±6.816 fg | 15.56 ± 7.698 j | 10.556 ± 7.083 b | 10.556 ± 7.083 b |
34 | 21°C | Jaga | J2+RD | 0 ± 0 i | 0 ± 0 k | 0 ± 0 g | 0 ± 0 f |
35 | 21°C | Jaga | J2+S+RD | 20.83 ± 6.376 f | 100 ± 0 a | 2.778 ±3.978 ef | 2.778 ± 3.978 de |
36 | 21°C | Jaga | J2+StS+RD | 18.33 ± 8.704 fg | 84.17 ± 24.168 b | 1.667 ± 2.659 fg | 1.667 ±2.659 ef |
LSD0.05 | 5.39 | 8.795 | 2.171 | 2.18 |
Source of variation | d.f. | Immobile J2 after 24 h | Immobile J2 after 48 h | Immobile J2 immersed in water | Immobile J2 after NaOH treatment |
---|---|---|---|---|---|
Temperature (T) | 2 | 8723.15*** | 38277.8*** | 943.39*** | 938.374*** |
Cultivar (C) | 1 | 40703.81*** | 8983.6*** | 257.202*** | 262.371*** |
Variant (V) | 5 | 9691.42*** | 34681.6*** | 583.498*** | 582.31*** |
TC | 2 | 159.67* | 3219.1*** | 47.557** | 47.016** |
TV | 10 | 3228.36*** | 7990.8*** | 440.458*** | 441.152*** |
CV | 5 | 5546.58*** | 2276.7*** | 184.671*** | 183.297*** |
TCV | 10 | 2071.8*** | 884.7*** | 104.192*** | 104.979*** |
Residual | 396 | 45.1 | 120.1 | 7.319 | 7.377 |
Primer | Seq 5´ to 3´- Forward | Seq 5´ to 3´ - Reverse |
---|---|---|
TCTCTGATGATGAGGCTGAAGA | TCACCGTCCTTCTTGTCCTT | |
ACTCATCTTGGTGGTGAAGATT | TCAATGCCATCAAAGAGAGAATCA | |
TTCCTGCTCTTGAATTGGCT | AATTGTGACTTCATCCGCCT | |
CGTAGAGAAGAATTCCGTGAAGA | TTCAGAGCGGTGACTTCCA | |
GCCTCTCCAGCATAATGACG | CGATTTATTTCACGACTGACTGA |
Trait | Immobile J2 after 24 h | Immobile J2 after 48 h | Immobile J2 immersed in water | Immobile J2 after NaOH treatment |
---|---|---|---|---|
Immobile J2 after 24 h | 1 | |||
Iimmobile J2 after 48 h | 0.7419*** | 1 | ||
Immobile J2 immersed in water | 0.2069 | 0.2241 | 1 | |
Immobile treatment J2 after NaOH | 0.2066 | 0.2241 | 1*** | 1 |
Hsp family | Gene |
Gene |
Gene location on contig |
---|---|---|---|
Hsp90 | MhA1_Contig1972.frz3.gene3 | 4732..7425 | |
Hsp70 | MhA1_Contig113.frz3.gene45 | 79493..86067 | |
Hsp60 | MhA1_Contig737.frz3.gene23 | 48523..50607 | |
sHsps | MhA1_Contig30.frz3.gene18 | 50262..54876 | |
sHsps | hsp12.3 (F38E11.1) | MhA1_Contig199.frz3.gene23 | 35708..36080 |