hemolymph outflow | After detachment of antennae | After incision of body with a scalpel | into a glass capillary by puncturing body | After detachment of antennae with glass capillary (modified method) |
---|---|---|---|---|
Difficulty level | easy | difficult | difficult and requiring precision | easy |
Microbiological contamination of collected material | likely | very likely | unlikely | unlikely |
Transport | very difficult | very difficult | difficult | easy |
Approximate amount of collected material | 5-10 μL | 2-8 μL | 6-20 μL | 8-20 μL |
Stage of insects used for hemolymph extraction | adult | adult and larvae | adult with developed circulatory system | adult |
Storage of collected material | difficult | difficult | easy | easy |
Collecting efficiency | fast | slow | slow | fast |
Possibility of hemocytes adhesion | no | no | yes | yes |