<abstract xmlns="http://www.w3.org/1999/xhtml"><p>In this report, we describe the initial colonization of environmental microorganisms associated with ISS on four different materials (Nomex, cable labeling material, printed circuit board, and aluminum), which are commonly used at the ISS. Material substrates were placed in the Russian segment of the ISS in a Target Book for 135 days. After the incubation, the Target Book was analyzed on Earth by determining colony-forming units and identifying the microorganisms by rRNA gene sequencing. The highest cell concentrations and widest biological diversity were on the polymer materials, such as Nomex and cable labeling material. Additional molecular biological identification revealed the following organisms as typical pioneer microorganisms: <italic>Staphylococcus</italic> spp., <italic>Bacillus</italic> spp., <italic>Streptococcus</italic> spp., <italic>Cladosporium</italic> spp., <italic>Sphingomonas</italic> spp., <italic>Micrococcus luteus</italic>, and <italic>Stenotrophomonas maltophilia</italic>.</p></abstract>

In this report, we describe the initial colonization of environmental microorganisms associated with ISS on four different materials (Nomex, cable labeling material, printed circuit board, and aluminum), which are commonly used at the ISS. Material substrates were placed in the Russian segment of the ISS in a Target Book for 135 days. After the incubation, the Target Book was analyzed on Earth by determining colony-forming units and identifying the microorganisms by rRNA gene sequencing. The highest cell concentrations and widest biological diversity were on the polymer materials, such as Nomex and cable labeling material. Additional molecular biological identification revealed the following organisms as typical pioneer microorganisms: Staphylococcus spp., Bacillus spp., Streptococcus spp., Cladosporium spp., Sphingomonas spp., Micrococcus luteus, and Stenotrophomonas maltophilia.

Study of Initial Colonization by Environmental Microorganisms in the Russian Segment of the International Space Station (ISS)

Department TX4 - Electronics, Communications and Intelligent Systems

Gravitational and Space Research

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

In this report, we describe the initial colonization of environmental microorganisms associated with ISS on four different materials (Nomex, cable labeling...

Study of Initial Colonization by Environmental Microorganisms in the Russian Segment of the International Space Station (ISS)

Article Category: Research Article

Pubblicato online: 18 gen 2022

Pagine: 46 - 57

DOI: https://doi.org/10.2478/gsr-2014-0012

© 2014 Ulrich Reidt et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.