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Extracellular activity of proteases from Yarrowia lipolytica IPS21 as a function of the carbon and nitrogen source

Dorota Wieczorek
Dorota Wieczorek
, 
Katarzyna Miśkiewicz
Katarzyna Miśkiewicz
, 
Dorota Gendaszewska
Dorota Gendaszewska
, 
Paulina Pipiak
Paulina Pipiak
, 
Magdalena Lasoń-Rydel
Magdalena Lasoń-Rydel
, 
Katarzyna Sieczyńska
Katarzyna Sieczyńska
 e 
Katarzyna Ławińska
Katarzyna Ławińska
   | 01 dic 2023
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Article Category: Research Article
Pubblicato online: 01 dic 2023
Pagine: 66 - 74
DOI: https://doi.org/10.2478/ftee-2023-0046
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© 2023 Łukasiewicz Research Network-Łódź Institute of Technology, published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Fig. 1.

*CTLS - chrome-tanned leather shavings Influence of the carbon source on the activity of the proteases of the yeast Y. lipolytica IPS21. Values for single elements are not significantly different (P < 0.05) according to Tukey’s Kramer test for multiple comparisons of supernatants marked with the same letter
*CTLS - chrome-tanned leather shavings Influence of the carbon source on the activity of the proteases of the yeast Y. lipolytica IPS21. Values for single elements are not significantly different (P < 0.05) according to Tukey’s Kramer test for multiple comparisons of supernatants marked with the same letter

Fig. 2.

*CTLS -chrome-tanned leather shavings Influence of the carbon source on the pH of the medium during the biological process with the yeast Y. lipolytica IPS21. Values for single elements are not significantly different (P < 0.05) according to Tukey’s Kramer test for multiple comparisons of the medium marked with the same letter
*CTLS -chrome-tanned leather shavings Influence of the carbon source on the pH of the medium during the biological process with the yeast Y. lipolytica IPS21. Values for single elements are not significantly different (P < 0.05) according to Tukey’s Kramer test for multiple comparisons of the medium marked with the same letter

Fig. 3.

*YE – yeast extract Influence of the amount of yeast extract in a medium with 0.6 g of CTLS on the activity of proteases of Y. lipolytica IPS21 yeast. Values for single elements are not significantly different (P < 0.05) according to Tukey’s Kramer test for multiple comparisons
*YE – yeast extract Influence of the amount of yeast extract in a medium with 0.6 g of CTLS on the activity of proteases of Y. lipolytica IPS21 yeast. Values for single elements are not significantly different (P < 0.05) according to Tukey’s Kramer test for multiple comparisons

Metal concentrations in selected samples of biomass with Y. lipolytica IPS21 yeast or a supernatant after the process of protease production in a medium with 0.6 g of CTLS and different amounts of yeast extract. Values for single elements are not significantly different (P<0.05) according to Tukey’s Kramer test for multiple comparisons of the biomass or supernatant marked with the same letter

MetalVariant Ca Cr Fe K Mg Na P S
g kg-1
0.2 g YE 3.16 ± 3.68 ± 0.55 ± 5.16 ± 2.44 ± 15.44 ± 16.02 ± 3.36 ±
biomass 0.21a 0.30a 0.02a 0.55a 0.13a 1.22a 1.45a 1.24a
0.4 g YE 1.92 ± 3.33 ± 0.51 ± 4.06 ± 2.12 ± 9.81 ± 19.89 ± 5.15 ±
biomass 0.11b 0.22a 0.02a 0.45b 0.18b 1.18b 2.66b 0.12b
0.2 g YE 0.10 ± 0.54 ± 0.09 ± 0.25 ± 0.05 ± 3.0 ± 0.09 ± 0.52 ±
supernatant 0.01a 0.01a 0.01a 0.08a 0.32a 0.54a 0.02a 0.13a
0.4 YE 0.10 ± 0.53 ± 0.10 ± 0.26 ± 0.06 ± 3.3 ± 0.08 ± 0.57 ±
supernatant 0.01a 0.02a 0.04a 0.02a 0.01a 0.03a 0.01a 0.03a

Influence of the carbon source on the biomass and yeast Y. lipolytica IPS21 protein concentration. Values for single elements are not significantly different (P < 0.05) according to Tukey’s Kramer test for multiple comparison of the biomass or total protein marked with the same letter

Carbon source Biomass production, g g-1 of carbohydrate substrate Total protein, μg mL-1
Yeast extract 0.385 ± 0.02d 120 ± 5.20a
Glucose 0.452 ± 0.11aa 124 ± 4.73a
Glycerol 0.465 ± 0.03aa 120 ± 6.55a
Starch 0.401 ± 0.02d 114 ± 4.66a
Semolina wheat 0.225 ± 0.09a 111 ± 7.22a
Oat 0.317 ± 0.12b 109 ± 1.99a
Saccharose 0.364 ± 0.09c 130 ± 2.33a
Pectin 0.299 ± 0.16b 128 ± 2.98a
Straw 0.364 ± 0.04c 112 ± 7.66a
Saccharose + CTLS* 0.354 ± 0.04c 124 ± 8.13a
Starch + CTLS 0.320 ± 0.08b 120 ± 5.78a
CTLS 0.204 ± 0.09a 110 ± 2.59a

Composition of amino acids in the supernatant after 48 hours of biological treatment with Y. lipolytica IPS21

Name of acid Yeast extract medium with Yarrowia CTLS medium with Yarrowia Yeast extract medium without Yarrowia CTLS medium without Yarrowia
g 100 g-1 of supernatant
Aspartic acid 0.03 0.22 0.07 0.13
Threonine <0.02 0.05 0.07 0.04
Serine <0.02 0.10 <0.02 0.06
Glutamic acid 0.09 0.29 0.09 0.26
Proline <0.02 0.33 0.03 0.25
Glycine <0.02 0.55 0.02 0.37
Alanine <0.02 0.21 0.06 0.20
Valine <0.02 0.05 0.05 0.06
Methionine <0.02 <0.02 <0.02 <0.02
Isoleucine <0.02 0.19 0.03 0.05
Leucine <0.02 0.08 0.06 0.08
Tyrosine <0.02 0.03 0.03 0.03
Phenylalanine <0.02 0.07 0.04 0.05
Ornithine <0.02 <0.02 <0.02 <0.02
Gamma-Aminobutyric acid <0.02 <0.02 <0.02 <0.02
Lysine <0.02 0.09 0.05 0.12
Histidine <0.02 0.03 <0.04 <0.02
Arginine <0.02 0.17 0.03 0.11
Taurine, 2-aminoethanesulfonic acid <0.02 <0.02 <0.02 <0.02
Hydroxyproline <0.02 0.30 <0.02 0.17
Cysteine <0.02 <0.02 <0.02 <0.02
Hydroxylysine <0.02 0.04 <0.02 <0.02
Sum of amino acid content (mean value) 0.12 2.78 0.63 1.98

Influence of the amount of yeast extract in the medium with 0.6 g of CTLS on the biomass and protein concentration of the yeast Y. lipolytica IPS21. Values for single elements are not significantly different (P<0.05) according to Tukey’s Kramer test for multiple comparisons of biomass or the total protein marked with the same letter

Carbon sources Biomass production, g g-1 of carbohydrate substrate Total protein, μg mL-1
0.6 g yeast extract 0.205 ± 0.14a 112.4 ± 6.55c
0.4 g yeast extract 0.375 ± 0.16c 89.2 ± 4.22b
0.2 g yeast extract 0.316 ± 0.09b 64.2 ± 4.33a
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