<abstract xmlns="http://www.w3.org/1999/xhtml">

The green bean (<italic>Phaseolus vulgaris</italic> L.) is a very widely grown food crop that contributes significantly to human dietary needs in many countries due to its high content of protein. This study evaluates foliar applications of ZnSO4 versus that of zinc oxide nanoparticles (ZnO NPs) to leaves of the green bean cv. ‘Strike’ and records the plant responses in terms of Zn uptake and concentrations of photosynthetic pigments and bioactive compounds. The experiment was conducted under greenhouse conditions in Chihuahua, Mexico, with a completely randomised experimental design with 10 replicates. The two treatments were foliar applications of either an aqueous solution of ZnSO4 or an aqueous suspension of ZnO NPs (both 150 mg · L−1). The application of ZnO NPs significantly increased concentrations of Zn2+ in the leaflets, roots, stems and pods of chlorophylls <italic>a</italic> and <italic>b</italic> (values 15.40 μg · g−1 and 11.64 μg · g−1, respectively). Sucrose concentration was also increased by Zn2+ applications, but no differences were found in total phenols (TP), total flavonoids (TFl) or antioxidant capacity (AC). In the pods and seeds, Zn2+ application left sucrose and TFl concentrations unchanged, but the TP increase was significant. The AC was affected by both zinc sources and only in the pods. The applications of ZnSO4 or ZnO NPs significantly increased the biomass accumulation (79.10 g · p−1 and 84.70 g · p−1 DW) and yield (55.64 g · p−1 and 53.80 g · p−1 FW). These results suggest that the application of ZnO NPs could represent a worthwhile biofortification strategy in the commercial production of green bean cv. ‘Strike’.
</abstract>

The green bean (Phaseolus vulgaris L.) is a very widely grown food crop that contributes significantly to human dietary needs in many countries due to its high content of protein. This study evaluates foliar applications of ZnSO4 versus that of zinc oxide nanoparticles (ZnO NPs) to leaves of the green bean cv. ‘Strike’ and records the plant responses in terms of Zn uptake and concentrations of photosynthetic pigments and bioactive compounds. The experiment was conducted under greenhouse conditions in Chihuahua, Mexico, with a completely randomised experimental design with 10 replicates. The two treatments were foliar applications of either an aqueous solution of ZnSO4 or an aqueous suspension of ZnO NPs (both 150 mg · L−1). The application of ZnO NPs significantly increased concentrations of Zn2+ in the leaflets, roots, stems and pods of chlorophylls a and b (values 15.40 μg · g−1 and 11.64 μg · g−1, respectively). Sucrose concentration was also increased by Zn2+ applications, but no differences were found in total phenols (TP), total flavonoids (TFl) or antioxidant capacity (AC). In the pods and seeds, Zn2+ application left sucrose and TFl concentrations unchanged, but the TP increase was significant. The AC was affected by both zinc sources and only in the pods. The applications of ZnSO4 or ZnO NPs significantly increased the biomass accumulation (79.10 g · p−1 and 84.70 g · p−1 DW) and yield (55.64 g · p−1 and 53.80 g · p−1 FW). These results suggest that the application of ZnO NPs could represent a worthwhile biofortification strategy in the commercial production of green bean cv. ‘Strike’.

Zinc sulphate or zinc nanoparticle applications to leaves of green beans

Instituto Tecnológico Nacional de México

Folia Horticulturae

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

{"article-title":"Zinc sulphate or zinc nanoparticle applications to leaves of green beans"}

The green bean (Phaseolus vulgaris L.) is a very widely grown food crop that contributes significantly to human dietary needs in many countries due to its high...

Treatment	Chl		TC	TAn	Sucrose	TP	TFl	AC

	a	b
Control	11.41 b	7.70 b	2.91 a	1.05 a	5.06 b	3.37 a	0.32 a	62.90 a
ZnSO₄	12.62 b	8.24 b	2.14 a	1.24 a	6.38 a	3.55 a	0.42 a	72.31 a
ZnO NPs	15.40 a	11.64 a	1.58 a	1.50 a	5.53 ab	3.20 a	0.44 a	56.57 a

Organ	Treatment	Sucrose (mg · g⁻¹)	TP (mg GAE · 100 g⁻¹)	TFl (mg CE · 100 g⁻¹)	AC (% DPPH inhibition)
Pod	Control	10.87 a	1.96 b	0.061 a	12.36 b
	ZnSO₄	10.89 a	1.80 b	0.074 a	18.60 a
	ZnO NPs	15.06 a	2.53 a	0.092 a	21.39 a

Seed	Control	12.90 a	2.18 b	0.109 a	71.15 a
	ZnSO₄	14.92 a	2.16 b	0.077 b	70.96 a
	ZnO NPs	17.87 a	4.15 a	0.136 a	70.48 a

Zinc sulphate or zinc nanoparticle applications to leaves of green beans

Article Category: Original Article

Pubblicato online: 28 dic 2021

Pagine: 365 - 375

Ricevuto: 12 mag 2021

Accettato: 29 nov 2021

DOI: https://doi.org/10.2478/fhort-2021-0028

Parole chiave
antioxidant capacity, efficiency, fertilisation, nanofertilisers, L.

© 2021 Jaime Bautista-Diaz et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

Changes in concentrations of photosynthetic pigments, bioactive compounds and antioxidant capacities in leaflets of the green bean cv. ‘Strike’ following applications of ZnSO4 and ZnO NPs.

Concentrations of sucrose, TP, TFl and AC in pods and seeds of the green bean cv. ‘Strike’ by supplying ZnSO4 and ZnO NPs.

Zinc sulphate or zinc nanoparticle applications to leaves of green beans

Article Category: Original Article

Pubblicato online: 28 dic 2021

Pagine: 365 - 375

Ricevuto: 12 mag 2021

Accettato: 29 nov 2021

DOI: https://doi.org/10.2478/fhort-2021-0028

Parole chiaveantioxidant capacity, efficiency, fertilisation, nanofertilisers, L.

© 2021 Jaime Bautista-Diaz et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

Changes in concentrations of photosynthetic pigments, bioactive compounds and antioxidant capacities in leaflets of the green bean cv. ‘Strike’ following applications of ZnSO4 and ZnO NPs.

Concentrations of sucrose, TP, TFl and AC in pods and seeds of the green bean cv. ‘Strike’ by supplying ZnSO4 and ZnO NPs.

Parole chiave
antioxidant capacity, efficiency, fertilisation, nanofertilisers, L.