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<p>This study examines genetic diversity and structure of a Croatian garlic germplasm collection using 13 simple sequence repeat (SSR) markers. A total of 71 alleles were observed across 64 accessions representing 3 Croatian regions (Istria, Dalmatia and continental Croatia) and 16 foreign landraces, with an average of 5.46 alleles per locus. Among the 80 accessions analysed, 61 distinct multilocus genotypes (MLG) were identified, of which 51 represented unique genotypes and the remaining accessions were divided into 10 MLG groups, comprising potential duplicates or redundant genotypes. Model-based Bayesian and hierarchical UPGMA clustering approaches revealed five major groups within the collection which partially correlated with geographical origin. The analysis of molecular variance (AMOVA) showed that the majority (87.71%) of the total molecular diversity is within the Croatian groups of accessions, even though a significant share (12.29%) of diversity derived from genetic diversity among groups. These results support regional structuring, as well as the existence of significant diversity within local populations. This study is the first comprehensive report on an extensive evaluation of genetic resources of garlic maintained by Croatia with the aim of setting the course for future preservation strategies with particular emphasis on the value of diversity in the context of climate change both on macro and micro levels.</p>
</abstract>

This study examines genetic diversity and structure of a Croatian garlic germplasm collection using 13 simple sequence repeat (SSR) markers. A total of 71 alleles were observed across 64 accessions representing 3 Croatian regions (Istria, Dalmatia and continental Croatia) and 16 foreign landraces, with an average of 5.46 alleles per locus. Among the 80 accessions analysed, 61 distinct multilocus genotypes (MLG) were identified, of which 51 represented unique genotypes and the remaining accessions were divided into 10 MLG groups, comprising potential duplicates or redundant genotypes. Model-based Bayesian and hierarchical UPGMA clustering approaches revealed five major groups within the collection which partially correlated with geographical origin. The analysis of molecular variance (AMOVA) showed that the majority (87.71%) of the total molecular diversity is within the Croatian groups of accessions, even though a significant share (12.29%) of diversity derived from genetic diversity among groups. These results support regional structuring, as well as the existence of significant diversity within local populations. This study is the first comprehensive report on an extensive evaluation of genetic resources of garlic maintained by Croatia with the aim of setting the course for future preservation strategies with particular emphasis on the value of diversity in the context of climate change both on macro and micro levels.

Genetic diversity and structure analysis of Croatian garlic collection assessed by SSR markers

University of Zagreb Faculty of Agriculture

Department of Genetics, Evolution, Microbiology and Immunology

Institute of Biology, University of Campinas (UNICAMP)

Folia Horticulturae

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

This study examines genetic diversity and structure of a Croatian garlic germplasm collection using 13 simple sequence repeat (SSR) markers. A total of 71...

Genetic diversity and structure analysis of Croatian garlic collection assessed by SSR markers

Article Category: Original Article

Pubblicato online: 13 mag 2021

Pagine: 157 - 171

Ricevuto: 15 dic 2020

Accettato: 31 mar 2021

DOI: https://doi.org/10.2478/fhort-2021-0011

Parole chiave
collections management, garlic, genetic diversity, genetic structure, plant genetic resources, SSR

© 2021 Danijela Poljuha et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Genetic diversity and structure analysis of Croatian garlic collection assessed by SSR markers

Article Category: Original Article

Pubblicato online: 13 mag 2021

Pagine: 157 - 171

Ricevuto: 15 dic 2020

Accettato: 31 mar 2021

DOI: https://doi.org/10.2478/fhort-2021-0011

Parole chiavecollections management, garlic, genetic diversity, genetic structure, plant genetic resources, SSR

© 2021 Danijela Poljuha et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Parole chiave
collections management, garlic, genetic diversity, genetic structure, plant genetic resources, SSR