<abstract xmlns="http://www.w3.org/1999/xhtml">

<p>The aim of this study was to compare three diagnostic methods for the diagnosis of cryptosporidiosis and to detect subtypes of<italic>Cryptosporidium parvum</italic> by sequences analyses of <italic>gp60</italic> gene in diarrheic calves in several herds in Konya province located in Central Anatolia Region of Turkey. Fecal samples were collected from a total of 194 pre-weaned calves (n=158, ≤15 days old, and n=36, 15 to 40 days old), with diarrhoea. For comparative diagnosis, all samples were examined by modified Ziehl-Neelsen staining of fecal smears for the presence of oocyst, nested PCR-RFLP of SSU rRNA and TaqMan qPCR for the detection of <italic>Cryptosporidium</italic> DNA. A total of 92 (47.4%) and 104 (53.6%) out of the examined samples were found positive by microscopic examination and molecular tools, respectively. The diagnostic sensitivity and specificity of microscopic identification were determined as 88.5% and 100.0%, respectively compared to molecular assays. <italic>Cryptosporidium parvum</italic> was the only detected species in all positive samples by species-specific qPCR and nested PCR-RFLP assays. Species identifications were further confirmed by sequence analyses of the SSU rRNA PCR products. There was no statistically significant difference in <italic>C. parvum</italic> prevalence between early pre-weaned calves and calves older than 15 days. The sequence analyses of the <italic>gp60</italic> gene of <italic>C. parvum</italic> isolates revealed a one subtype IIaA13G2R1 belonging to zoonotic family IIa in diarrheic calves</p></abstract>

The aim of this study was to compare three diagnostic methods for the diagnosis of cryptosporidiosis and to detect subtypes ofCryptosporidium parvum by sequences analyses of gp60 gene in diarrheic calves in several herds in Konya province located in Central Anatolia Region of Turkey. Fecal samples were collected from a total of 194 pre-weaned calves (n=158, ≤15 days old, and n=36, 15 to 40 days old), with diarrhoea. For comparative diagnosis, all samples were examined by modified Ziehl-Neelsen staining of fecal smears for the presence of oocyst, nested PCR-RFLP of SSU rRNA and TaqMan qPCR for the detection of Cryptosporidium DNA. A total of 92 (47.4%) and 104 (53.6%) out of the examined samples were found positive by microscopic examination and molecular tools, respectively. The diagnostic sensitivity and specificity of microscopic identification were determined as 88.5% and 100.0%, respectively compared to molecular assays. Cryptosporidium parvum was the only detected species in all positive samples by species-specific qPCR and nested PCR-RFLP assays. Species identifications were further confirmed by sequence analyses of the SSU rRNA PCR products. There was no statistically significant difference in C. parvum prevalence between early pre-weaned calves and calves older than 15 days. The sequence analyses of the gp60 gene of C. parvum isolates revealed a one subtype IIaA13G2R1 belonging to zoonotic family IIa in diarrheic calves

Comparison of three diagnostic methods in the diagnosis of cryptosporidiosis and <italic>gp</italic>60 subtyping of <italic>Cryptosporidium parvum</italic> in diarrheic calves in Central Anatolia Region of Turkey

The EuroBiotech Journal

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Comparison of three diagnostic methods in the diagnosis of cryptosporidiosis and 60 subtyping of  in diarrheic calves in Central Anatolia Region of Turkey

The aim of this study was to compare three diagnostic methods for the diagnosis of cryptosporidiosis and to detect subtypes ofCryptosporidium parvum by...

Method		Molecular tools		Sensitivity	Specificity
Method		Positive	Negative	Sensitivity	Specificity
Microscopy	Positive	92	0	85.5%	100%
Microscopy	Negative	12	90	(80.7-93.9%)	(96.0-100.0%)
Total		104	90

Age group (days)	Total no. of samples	Cryptosporidium parvum
Age group (days)	Total no. of samples	No. positive (%)	χ2	p	gp60 subtype
Early pre-weaned calves (≤ 15)	158	89 (56.3)	2.535	0.139	IIaA13G2R1
Pre-weaned calves (15-40)	36	15 (41.7)
Total	194	104 (53.6)

Comparison of three diagnostic methods in the diagnosis of cryptosporidiosis and gp60 subtyping of Cryptosporidium parvum in diarrheic calves in Central Anatolia Region of Turkey

Article Category: Research Article

Pubblicato online: 22 apr 2021

Pagine: 63 - 69

DOI: https://doi.org/10.2478/ebtj-2021-0010

Parole chiave
diarrheic calves, subtype, nested PCR-RFLP, prevalence, TaqMan qPCR, Turkey

© 2021 Alparslan Yildirim, Ferda Sevinc, Zuhal Onder, Onder Duzlu, Ozlem Derinbay Ekici, Nermin Isik, Arif Ciloglu, Emrah Simsek, Gamze Yetismis, Abdullah Inci, published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Figure 1

Comparison of the results of microscopic examination and molecular assays in the detection of Cryptosporidium in calves and evaluation of diagnostic sensitivity and specificity.

Distribution and subtype of C. parvum over age groups of the examined calves based on molecular diagnostic tests used in the study.

Comparison of three diagnostic methods in the diagnosis of cryptosporidiosis and gp60 subtyping of Cryptosporidium parvum in diarrheic calves in Central Anatolia Region of Turkey

Article Category: Research Article

Pubblicato online: 22 apr 2021

Pagine: 63 - 69

DOI: https://doi.org/10.2478/ebtj-2021-0010

Parole chiavediarrheic calves, subtype, nested PCR-RFLP, prevalence, TaqMan qPCR, Turkey

© 2021 Alparslan Yildirim, Ferda Sevinc, Zuhal Onder, Onder Duzlu, Ozlem Derinbay Ekici, Nermin Isik, Arif Ciloglu, Emrah Simsek, Gamze Yetismis, Abdullah Inci, published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Figure 1

Comparison of the results of microscopic examination and molecular assays in the detection of Cryptosporidium in calves and evaluation of diagnostic sensitivity and specificity.

Distribution and subtype of C. parvum over age groups of the examined calves based on molecular diagnostic tests used in the study.

Parole chiave
diarrheic calves, subtype, nested PCR-RFLP, prevalence, TaqMan qPCR, Turkey