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Genetic bases of tomatо marker-assisted selection in Belarus

Olga G. Babak
Olga G. Babak
, 
Natalia A. Nekrashevich
Natalia A. Nekrashevich
, 
Konstantsia K. Yatsevich
Konstantsia K. Yatsevich
, 
Sergey V. Malyshev
Sergey V. Malyshev
 e 
Alexander V. Kilchevsky
Alexander V. Kilchevsky
   | 25 apr 2018
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Article Category: Research Article
Pubblicato online: 25 apr 2018
Pagine: 128 - 135
DOI: https://doi.org/10.2478/ebtj-2018-0017
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© 2018 Olga G. Babak, Natalia A. Nekrashevich, Konstantsia K. Yatsevich, Sergey V. Мalyshev, Alexander V. Kilchevsky, published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Figure 1

PCR amplification results with tF/ tR SCAR marker. Lines: 1,2 - LA3134, 3,4 - LA2529, 5,6,7 - LA2374, 8, 9,10- LA3899, 11,12,13 - LA4062, 14,15,16 - LA3183, 17,18,19 - LA348.
PCR amplification results with tF/ tR SCAR marker. Lines: 1,2 - LA3134, 3,4 - LA2529, 5,6,7 - LA2374, 8, 9,10- LA3899, 11,12,13 - LA4062, 14,15,16 - LA3183, 17,18,19 - LA348.

Figure 2

PCR amplification results with OgcF/ OgcR SCAR marker. А - Polyacrylamide gel. Lines: 1-2, 9-10 – with Ogc allele, B - fragment analysis on an automatic sequencer: homo- and heterozygous samples.
PCR amplification results with OgcF/ OgcR SCAR marker. А - Polyacrylamide gel. Lines: 1-2, 9-10 – with Ogc allele, B - fragment analysis on an automatic sequencer: homo- and heterozygous samples.

Figure 3

PCR amplification results with BpromF/BpromR SCAR marker. А - Polyacrylamide gel. Lines: 1-2, 7-8 – with b allele, 3-4, 5-6 – with B allele B – fragment analysis on an automatic sequencer: homo- and heterozygous samples.
PCR amplification results with BpromF/BpromR SCAR marker. А - Polyacrylamide gel. Lines: 1-2, 7-8 – with b allele, 3-4, 5-6 – with B allele B – fragment analysis on an automatic sequencer: homo- and heterozygous samples.

Figure 4

PCR amplification results with the SCAR marker RinF1/ RinwR1/RinmR2. Lines: 1-3 – L154, 4,5 – Mо 577 (rin), 6 -8 –F1 hybrid (L154 х Мо 577).
PCR amplification results with the SCAR marker RinF1/ RinwR1/RinmR2. Lines: 1-3 – L154, 4,5 – Mо 577 (rin), 6 -8 –F1 hybrid (L154 х Мо 577).

Figure 5

Results of PCR amplification of DNA tomato forms with NorF/ NorR SCAR marker: 1, 2 –Mo 948 (nor), 3, 4 – Мо 950 (norA), 5, 6 – Line 19/1 (norwt ) (А); Results of fragment analysis of plants F2: homozygous plant (nor/nor) (B) and heterozygous plant (nor/norwt) (C).
Results of PCR amplification of DNA tomato forms with NorF/ NorR SCAR marker: 1, 2 –Mo 948 (nor), 3, 4 – Мо 950 (norA), 5, 6 – Line 19/1 (norwt ) (А); Results of fragment analysis of plants F2: homozygous plant (nor/nor) (B) and heterozygous plant (nor/norwt) (C).

Figure 6

A fragment of the DNA-sequence of the second exon of the LeNAC-NOR gene in the norA mutant and the wild allele. The arrow shows the position of the point mutation in the norA allele.t (nor/norwt) (C).
A fragment of the DNA-sequence of the second exon of the LeNAC-NOR gene in the norA mutant and the wild allele. The arrow shows the position of the point mutation in the norA allele.t (nor/norwt) (C).

Figure 7

Restriction products of the CAPS marker (dAlc-BspMII) used for norA allele identification. Lines:1 – La 0059 (norwt), 2 – Mo 948 (nor), 3 –Mo 950 (norA), 4 – La2529 (norA), 5 – La3134 (norA), 6 –Mo 577 (rin), 7 – F1norA/norwt.
Restriction products of the CAPS marker (dAlc-BspMII) used for norA allele identification. Lines:1 – La 0059 (norwt), 2 – Mo 948 (nor), 3 –Mo 950 (norA), 4 – La2529 (norA), 5 – La3134 (norA), 6 –Mo 577 (rin), 7 – F1norA/norwt.

Figure 8

The results of DNA amplification of tomato genotypes with the gf-3F/gf-3R marker: А. Polyacrylamide gel. Lines: 1, 2 – Black Prince (gf-3), 3, 4 – Black cherry, 5, 6 – Noire charbonneuse; B. Fragment analysis on an automatic sequencer: homo- and heterozygous samples.
The results of DNA amplification of tomato genotypes with the gf-3F/gf-3R marker: А. Polyacrylamide gel. Lines: 1, 2 – Black Prince (gf-3), 3, 4 – Black cherry, 5, 6 – Noire charbonneuse; B. Fragment analysis on an automatic sequencer: homo- and heterozygous samples.

Figure 9

The results of DNA amplification of tomato in F3 population genotypes with hp-2dg F/hp-2dg R and subsequent restriction by AclI. № 1-10, 12 – homozygous hp-2dg form, No. 14, 16, 17 – heterozygous form, No. 11, 13, 18 – samples with wild allele.
The results of DNA amplification of tomato in F3 population genotypes with hp-2dg F/hp-2dg R and subsequent restriction by AclI. № 1-10, 12 – homozygous hp-2dg form, No. 14, 16, 17 – heterozygous form, No. 11, 13, 18 – samples with wild allele.

Figure 10

Forms of tomato with various structural and regulatory genes: ogc/hp2dg, B/hp2dg, B/gf-3, B/hp2dg/nor, ogc/gf-3/rin.
Forms of tomato with various structural and regulatory genes: ogc/hp2dg, B/hp2dg, B/gf-3, B/hp2dg/nor, ogc/gf-3/rin.

Figure 11

Peculiarities of the carotenoid accumulation in tomato forms with ogc allele. Genotypes of the analysed tomato forms: 1 – ogc/ogc//rinwt/rinwt, 2 – ogc/ogc//rin/rin, 3 – ogc/ogc//gf-3/gf-3//rinwt/rinwt, 4 – ogc/ogc//gf-3/gf-3//rin/rin, 5 – ogc/ogc//hp2dg/hp2 dg//rinwt/rinwt, 6 – ogc/ogc//hp2dg/hp2dg//rin/rin, 7 – ogc/ogc/nor wt/norwt, 8 – ogc/ogc/nor /nor, 9 – ogc/ogc//gf-3/gf-3//nor/nor, 10 – ogc/ogc//hp2dg/hp2 dg//norwt/norwt, 11 – ogc/ogc//hp2dg/hp2 dg//nor/nor.
Peculiarities of the carotenoid accumulation in tomato forms with ogc allele. Genotypes of the analysed tomato forms: 1 – ogc/ogc//rinwt/rinwt, 2 – ogc/ogc//rin/rin, 3 – ogc/ogc//gf-3/gf-3//rinwt/rinwt, 4 – ogc/ogc//gf-3/gf-3//rin/rin, 5 – ogc/ogc//hp2dg/hp2 dg//rinwt/rinwt, 6 – ogc/ogc//hp2dg/hp2dg//rin/rin, 7 – ogc/ogc/nor wt/norwt, 8 – ogc/ogc/nor /nor, 9 – ogc/ogc//gf-3/gf-3//nor/nor, 10 – ogc/ogc//hp2dg/hp2 dg//norwt/norwt, 11 – ogc/ogc//hp2dg/hp2 dg//nor/nor.

Figure 12

Peculiarities of the carotenoid accumulation in tomato forms with B allele. Genotypes of the analysed tomato forms: 1 – B/B//rinwt/rinwt, 2 – B/B//rin/rin, 3 – B/B//gf-3/gf-3//rinwt/rinwt, 4 – B/B//gf-3/gf-3//rin/rin, 5 – B/B//hp2dg/hp2dg//rinwt/rinwt, 6 – B/B//hp2dg/hp2dg//rin/rin, 7 – B/B//norwt/norwt, 8 – B/B//nor/nor, 9 – B/B//gf-3/gf-3//nor/nor, 10 – B/B//hp2dg/hp2dg//norwt/norwtt, 11 – B/B//hp2dg/hp2dg//nor/nor.
Peculiarities of the carotenoid accumulation in tomato forms with B allele. Genotypes of the analysed tomato forms: 1 – B/B//rinwt/rinwt, 2 – B/B//rin/rin, 3 – B/B//gf-3/gf-3//rinwt/rinwt, 4 – B/B//gf-3/gf-3//rin/rin, 5 – B/B//hp2dg/hp2dg//rinwt/rinwt, 6 – B/B//hp2dg/hp2dg//rin/rin, 7 – B/B//norwt/norwt, 8 – B/B//nor/nor, 9 – B/B//gf-3/gf-3//nor/nor, 10 – B/B//hp2dg/hp2dg//norwt/norwtt, 11 – B/B//hp2dg/hp2dg//nor/nor.

Carotenoid content in tomato samples with tangerine and non-ripening alleles (mg/100 g wet basis), 2016

Allele combinationsneurosporinζ - carotene
t/t//norwt/norwt11.60
t/t//norA/norA07.2
t/t//nor/nor1.940.94

The content of carotenoids in tomato samples with different combinations of quality alleles (mg/100 g wet basis), 2016

Allele combinationsβ- caroteneLycopeneLuteinChlorophyll
ab
ogc/ogc//rinwt/rinwt0.8511.0000
ogc/ogc//rin/rin0.4400.2600
ogc/ogc//gf-3/gf-3//rinwt/rinwt0.555.75000
ogc/ogc//gf-3/gf-3//rin/rin0,250.8000
ogc/ogc//hp2dg/hp2 dg//rinwt/rinwt1.0516.92.200
ogc/ogc//hp2dg/hp2 dg//rin/rin0.701.10.600.62
ogc/ogc//norwt/norwt1.158.7000
ogc/ogc//nor/nor0.71.9000
ogc/ogc//gf-3/gf-3//nor/nor0.833.40.570.280.28
ogc/ogc//hp2dg/hp2dg//norwt/norwt1.719.92.200
ogc/ogc//hp2dg/hp2dg//nor/nor1.40.71.400
B/B//rinwt/rinwt2.91.01.200
B/B//rin/rin0.6900.600
B/B//gf-3/gf-3//rinwt/rinwt3.12.1000
B/B//gf-3/gf-3//rin/rin0.600.250.870.93
B/B//hp2dg/hp2 dg//rinwt/rinwt5.502.1500
B/B//hp2dg/hp2 dg//rin/rin0.600.70.680.63
B/B//norwt/norwt4.71.430.7500
B/B//gf-3/gf-3//norwt/norwt1.85.51.03.02.1
B/B//gf-3/gf-3//nor/nor0.28002.32.6
B/B//hp2dg/hp2 dg//norwt/norwt5.101.000
B/B//hp2dg/hp2 dg//nor/nor1.630.31.21.010.80
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