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<bold>Introduction.</bold> <italic>Haemophilus influenzae</italic> and <italic>Haemophilus parainfluenzae</italic> are known as human-restricted respiratory microbiota representatives. The aim of the present paper was to assay haemophili prevalence in middle ear effusion specimens in pediatric patients with otitis media with effusion (OME).
<bold>Methods.</bold> A total of 86 ear effusion specimens (from the left and right ear independently) were collected from 43 pediatric patients with OME. For comparison, 58 nasopharyngeal specimens were taken from 58 pediatric patients prone to recurrent respiratory tract infections (RRTI). Isolation and identification of haemophili biotypes and antimicrobial susceptibility was accomplished by standard microbiological methods. The cell surface hydrophobicity (CSH) of isolates was assayed by the method of aggregation in ammonium sulfate (SAT).
<bold>Results.</bold> Haemophili were isolated in 25.6% (11/43) of all OME patients: in 5/43 (11.6%)– <italic>H. influenzae</italic> (biotypes II, III), in 5/43 (11.6%) <italic>– H. parainfluenzae</italic>, in 1/43 (2.3%) – both species were found. Haemophili-positive nasopharyngeal specimen was found in 27/58 (46.6%) RRTI patients: in 19/58 (32.8%) – H. <italic>influenzae</italic>, in 8/58 (13.8%) – <italic>H</italic>. <italic>parainfluenzae</italic>. About 90% of all haemophili isolates were characterised by extreme to strong CSH. Antimicrobial resistance occurred mainly among <italic>H. parainfluenzae</italic> (80%) and to a much lower percentage among <italic>H. influenzae</italic> (33.3%) isolates. The obtained data suggest that both <italic>H. influenzae</italic> and <italic>H. parainfluenzae</italic> can be involved in pathology of OME in pediatric patients. The high cell surface hydrophobicity can affect on the haemophili prevalence and ear colonization, and induces predisposition to the presence of these bacteria as a biofilm that serves as a virulence factor with great importance for the survival of these opportunistic bacteria and their persistence in the ear environment.
</abstract>

Introduction. Haemophilus influenzae and Haemophilus parainfluenzae are known as human-restricted respiratory microbiota representatives. The aim of the present paper was to assay haemophili prevalence in middle ear effusion specimens in pediatric patients with otitis media with effusion (OME).
Methods. A total of 86 ear effusion specimens (from the left and right ear independently) were collected from 43 pediatric patients with OME. For comparison, 58 nasopharyngeal specimens were taken from 58 pediatric patients prone to recurrent respiratory tract infections (RRTI). Isolation and identification of haemophili biotypes and antimicrobial susceptibility was accomplished by standard microbiological methods. The cell surface hydrophobicity (CSH) of isolates was assayed by the method of aggregation in ammonium sulfate (SAT).
Results. Haemophili were isolated in 25.6% (11/43) of all OME patients: in 5/43 (11.6%)– H. influenzae (biotypes II, III), in 5/43 (11.6%) – H. parainfluenzae, in 1/43 (2.3%) – both species were found. Haemophili-positive nasopharyngeal specimen was found in 27/58 (46.6%) RRTI patients: in 19/58 (32.8%) – H. influenzae, in 8/58 (13.8%) – H. parainfluenzae. About 90% of all haemophili isolates were characterised by extreme to strong CSH. Antimicrobial resistance occurred mainly among H. parainfluenzae (80%) and to a much lower percentage among H. influenzae (33.3%) isolates. The obtained data suggest that both H. influenzae and H. parainfluenzae can be involved in pathology of OME in pediatric patients. The high cell surface hydrophobicity can affect on the haemophili prevalence and ear colonization, and induces predisposition to the presence of these bacteria as a biofilm that serves as a virulence factor with great importance for the survival of these opportunistic bacteria and their persistence in the ear environment.

<italic>Haemophilus influenzae</italic> and <italic>Haemophilus parainfluenzae</italic> occurrence in the ear effusion in pediatric patients prone to recurrent respiratory tract infections (RRTI) and with otitis media with effusion (OME)

Department of Pharmaceutical Microbiology

Department of Pediatric Otolaryngology, Phoniatrics and Audiology

Current Issues in Pharmacy and Medical Sciences

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

 and  occurrence in the ear effusion in pediatric patients prone to recurrent respiratory tract infections (RRTI) and with otitis media with effusion (OME)

Introduction. Haemophilus influenzae and Haemophilus parainfluenzae are known as human-restricted respiratory microbiota representatives. The aim of the...

Haemophilus influenzae and Haemophilus parainfluenzae occurrence in the ear effusion in pediatric patients prone to recurrent respiratory tract infections (RRTI) and with otitis media with effusion (OME)

Pubblicato online: 31 dic 2019

Pagine: 183 - 188

Ricevuto: 06 mag 2019

Accettato: 30 set 2019

DOI: https://doi.org/10.2478/cipms-2019-0032

Parole chiave
Haemophilus influenzae, Haemophilus parainfluenzae, pediatric patients, otitis media with effusion, bacterial cell surface hydrophobicity

© 2019 Urszula Kosikowska et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Haemophilus influenzae and Haemophilus parainfluenzae occurrence in the ear effusion in pediatric patients prone to recurrent respiratory tract infections (RRTI) and with otitis media with effusion (OME)

Pubblicato online: 31 dic 2019

Pagine: 183 - 188

Ricevuto: 06 mag 2019

Accettato: 30 set 2019

DOI: https://doi.org/10.2478/cipms-2019-0032

Parole chiaveHaemophilus influenzae, Haemophilus parainfluenzae, pediatric patients, otitis media with effusion, bacterial cell surface hydrophobicity

© 2019 Urszula Kosikowska et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Parole chiave
Haemophilus influenzae, Haemophilus parainfluenzae, pediatric patients, otitis media with effusion, bacterial cell surface hydrophobicity