<abstract xmlns="http://www.w3.org/1999/xhtml"><p>We experimentally carried out the synthesis of the tbf gene that encodes highly-immunogenic epitopes of pathogenic cholera strains, through the cloning of the tbf gene in pGEM-T Easy plasmid. Moreover, we tested the hybrid gene sequence for absence of mutations, using the Sanger sequencing. We also calculated the nucleatic sequence of the tbf gene. The obtained results have both scientific and practical significance.</p></abstract>

We experimentally carried out the synthesis of the tbf gene that encodes highly-immunogenic epitopes of pathogenic cholera strains, through the cloning of the tbf gene in pGEM-T Easy plasmid. Moreover, we tested the hybrid gene sequence for absence of mutations, using the Sanger sequencing. We also calculated the nucleatic sequence of the tbf gene. The obtained results have both scientific and practical significance.

Obtaining the tbf gene which encodes immunodominant epitopes of pathogenic cholera strains

Peter the Great Saint Petersburg State Polytechnical University

Current Issues in Pharmacy and Medical Sciences

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

We experimentally carried out the synthesis of the tbf gene that encodes highly-immunogenic epitopes of pathogenic cholera strains, through the cloning of the...

Obtaining the tbf gene which encodes immunodominant epitopes of pathogenic cholera strains

Pubblicato online: 28 set 2019

Pagine: 130 - 133

Ricevuto: 06 nov 2018

Accettato: 08 feb 2019

DOI: https://doi.org/10.2478/cipms-2019-0024

Parole chiave
candidate cholera vaccine, hybrid tbf gene, Sanger sequencing, protein design, TBF recombinant protein, immunodominant epitopes,

© 2019 Yulia Bazarnova et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Obtaining the tbf gene which encodes immunodominant epitopes of pathogenic cholera strains

Pubblicato online: 28 set 2019

Pagine: 130 - 133

Ricevuto: 06 nov 2018

Accettato: 08 feb 2019

DOI: https://doi.org/10.2478/cipms-2019-0024

Parole chiavecandidate cholera vaccine, hybrid tbf gene, Sanger sequencing, protein design, TBF recombinant protein, immunodominant epitopes,

© 2019 Yulia Bazarnova et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Parole chiave
candidate cholera vaccine, hybrid tbf gene, Sanger sequencing, protein design, TBF recombinant protein, immunodominant epitopes,