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New in vitro findings about halogenated boroxine cytotoxicity and deregulation of cell death-related genes in GR-M melanoma cells

Nikolina Elez-Burnjaković
Nikolina Elez-Burnjaković
, 
Lejla Pojskić
Lejla Pojskić
, 
Anja Haverić
Anja Haverić
, 
Naida Lojo-Kadrić
Naida Lojo-Kadrić
, 
Maida Hadžić Omanović
Maida Hadžić Omanović
, 
Jasmin Ramić
Jasmin Ramić
, 
Ajla Smajlović
Ajla Smajlović
, 
Milka Maksimović
Milka Maksimović
 e 
Sanin Haverić
Sanin Haverić
   | 04 apr 2023
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Article Category: Original article
Pubblicato online: 04 apr 2023
Pagine: 16 - 21
Ricevuto: 01 dic 2022
Accettato: 01 feb 2023
DOI: https://doi.org/10.2478/aiht-2023-74-3702
© 2023 Nikolina Elez-Burnjaković et al., published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Figure 1

Growth inhibition of GR-M and PBM cells treated with different concentrations of halogenated boroxine; *p<0.05
Growth inhibition of GR-M and PBM cells treated with different concentrations of halogenated boroxine; *p<0.05

Primer sequences used for selected cell death-related genes

Gene Reference sequence No. (FASTA format) Primers
BECN1 NM_003766.5 Forward: CTCCCGAGGTGAAGAGCATCReverse: GGGGGATGAATCTGCGAGAG
SQSTM1 NM_003900.5 Forward: CCGTGAAGGCCTACCTTCTGReverse: TCCTCGTCACTGGAAAAGGC
BCL-2 NM_000633.3 Forward: GGGGTCATGTGTGTGGAGAGReverse: GAAATCAAACAGAGGCCGCA
DRAM1 NM_018370.3 Forward: TTGGTGCAGCCACGATGTATReverse: ACACCACAGACAAAGGCCAA

Changes in relative BCL-2 gene expression after GR-M and PBM cell treatment with halogenated boroxine compared to negative control

HB concentrations (mg/mL) GR-M cells PBM cells

Fold change/P value

Fold change/P value
0.05 down -3.805/0.672 up 2.04/0.001
0.1 up 11.769/0.001 down -1.158/0.677
0.2 up 1.568/0.001 up 2.14/0.001
0.4 down -10.933/0.001 down -1.262/0.169
0.8 down -1.041/0.848 up 6.365/0.001

Changes in relative DRAM1 gene expression after GR-M and PBM cell treatment with halogenated boroxine compared to negative control

HB concentrations (mg/mL) GR-M cells PBM cells

Fold change/P value

Fold change/P value
0.05 down 1.661/0.3405 up 15.778/0.667
0.1 down 1.098/0.671 up 459.792/0.001
0.2 down 1.209/0.828 up 7968.193/0.001
0.4 down 4.195/0.001 up 6014.928/0.001
0.8 down 2.344/0.001 / /

Changes in relative BECN1 gene expression after GR-M and PBM cell treatment with halogenated boroxine compared to negative control

HB concentrations (mg/mL) GR-M cells PBM cells

Fold change/P value

Fold change/P value
0.05 up 1.788/0.001 up 3.884/0.001
0.1 down 2.143/0.0815 up 1.848/0.001
0.2 up 2.202/0.322 up 15.735/0.001
0.4 up 1.04/0.83 up 62.542/0.001
0.8 down 3.188/0.001 up 72636.933/0.001

Changes in relative SQSTM1 gene expression after GR-M and PBM cell treatment with halogenated boroxine compared to negative control

HB concentrations (mg/mL) GR-M cells PBM cells

Fold change/P value

Fold change/P value
0.05 up 1.387/0.1695 down 1.078/0.6465
0.1 up 3.643/0.001 down 1.951/0.153
0.2 up 11.612/0.001 up 5.764/0.001
0.4 up 6.92/0.001 up 7.424/0.001
0.8 up 3.295/0.001 up 1.004/0.6465
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Lingue:
Inglese, Slovenian
Frequenza di pubblicazione:
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Argomenti della rivista:
Medicine, Basic Medical Science, other
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