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Archives of Industrial Hygiene and Toxicology
Volume 71 (2020): Numero 4 (December 2020)
Accesso libero
Yellow gentian root extract provokes concentration- and time-dependent response in peripheral blood mononuclear cells
Ana Valenta Šobot
Ana Valenta Šobot
,
Dunja Drakulić
Dunja Drakulić
,
Gordana Joksić
Gordana Joksić
,
Jadranka Miletić Vukajlović
Jadranka Miletić Vukajlović
,
Jasmina Savić
Jasmina Savić
,
Jelena Potočnik
Jelena Potočnik
e
Jelena Filipović Tričković
Jelena Filipović Tričković
| 31 dic 2020
Archives of Industrial Hygiene and Toxicology
Volume 71 (2020): Numero 4 (December 2020)
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Article Category:
Original article
Pubblicato online:
31 dic 2020
Pagine:
320 - 328
Ricevuto:
01 ago 2020
Accettato:
01 ott 2020
DOI:
https://doi.org/10.2478/aiht-2020-71-3476
© 2020 Ana Valenta Šobot, Dunja Drakulić, Gordana Joksić, Jadranka Miletić Vukajlović, Jasmina Savić, Jelena Potočnik, Jelena Filipović Tričković published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
Figure 1
Particle size distribution of dispersed particles in (a) yellow gentian root powder; (b) lyophilisate; and (c) dissolved lyophilisate (pre-filtrate). Scale bar – 100 μm
Figure 2
UPLC chromatograms of yellow gentian root extract in ethanol solution exported at 254 nm (a); 300 nm (b); 270 nm (c); and 240 nm (d). 1 – loganic acid; 2 – swertiamarin; 3 – overlapping gentiopicroside and sweroside peaks
Figure 3
Cell viability expressed as percentage of control (a) and percentage of chromosome breaks (b) at different concentrations of yellow gentian root extract after 48 and 72 h treatment. *p<0.05, **p<0.01, ***p<0.001
Figure 4
Photomicrograph of metaphase spreads after 72 h treatment with 2 mg/mL of yellow gentian root extract. Arrows mark chromatid break (a) and radial figure between two homologous chromosomes (17q) an indicator of homologous recombination - a mechanism of template-dependent DNA DSBs repair (b)
Figure 5
Graph (a) and photomicrograph (b-e) of YGRE-induced DNA damage established with the comet assay. b – control; c – 0.5 mg/mL; d – 1 mg/mL; e – 2 mg/mL; YGRE – yellow gentian root extract. Scale bar – 10 μm. ***p<0.001
Figure 6
PAB levels presented as arbitrary HK units (a) and LPP levels expressed as nmol/mg of proteins (b) after treatment with increasing concentrations of yellow gentian root extract. ***p<0.001
Gradient condition details used for UPLC separation.
time (min)
% B
0 → 2
10 → 28
2 → 3.5
28 → 30
3.5 → 5
30 → 55
5 → 5.1
55 → 10
5.1 → 6
10 →