Effect of cisplatin on lipid peroxidation in the whole blood and plasma of female rats

Changes in concentration of malondialdehyde and of catalase enzyme activity in blood of female rats_

Concentration of malondialdehyde (nmol/ml)
	Baseline without treatment	After cisplatin action
Whole blood	6.62 ± 0.32	16.30 ± 0.70*
Blood plasma	4.62 ± 0.22	7.31 ± 0.25*
Activity of catalase enzyme in μmol of H2O2/mg protein min
Blood plasma	517.00 ± 8.50	233.00 ± 4.60*

The amount of lipid peroxidation products (in conventional units/1 ml of blood or plasma) in isopropyl alcohol phase extracted from the whole blood and plasma of rats of control group and animals injected with cisplatin_

Lipid peroxidation products	Isopropyl alcohol phase
	Whole blood		Blood plasma
	Baseline without treatment	After cisplatin action	Baseline without treatment	After cisplatin action
Unsaturated fatty acids	33.05 ± 0.58	56.00 ± 2.40*	22.00 ± 1.30	27.75 ± 0.23*
Conjugated dienes	9.00 ± 0.20	17.38 ± 0.34*	6.88 ± 0.25	8.70 ± 0.31*
Conjugated trienes	7.58 ± 0.14	12.10 ± 0.30*	3.54 ± 0.05	4.40 ± 0.20*

The amount of lipid peroxidation products (in conventional units/1 ml of blood or plasma) in heptane phase extracted from the whole blood and plasma of rats of the control group and animals injected with cisplatin

Lipid peroxidation products	Heptane phase
	Whole blood		Blood plasma
	Baseline without treatment	After cisplatin action	Baseline without treatment	After cisplatin action
Unsaturated fatty acids	41.00 ± 1.64	45.00 ± 1.15	29.27 ± 1.20	27.41 ± 0.78
Conjugated dienes	4.52 ± 0.13	7.15 ± 0.10*	6.44 ± 0.35	8.84 ± 0.14*
Conjugated trienes	1.63 ± 0.10	3.27 ± 0.16*	2.45 ± 0.15	4.00 ± 0.11*

Oxidation index for lipid peroxidation products, conjugated dienes and trienes, from the heptane and isopropyl alcohol phases of whole blood and plasma at baseline and after 24-h exposure to cisplatin_

Oxidation index	Phases of lipid peroxidation products	Whole blood		Blood plasma
		Baseline without treatment	After cisplatin action	Baseline without treatment	After cisplatin action
For conjugated dienes (D232/D220)	Heptane phase	0.110	0.158	0.220	0.323
	Isopropyl alcohol phase	0.272	0.310	0.313	0.314
For conjugated trienes (D278/D220)	Heptane phase	0.040	0.073	0.084	0.146
	Isopropyl alcohol phase	0.230	0.216	0.161	0.159