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Spheroids as 3D Cell Models for Testing of Drugs

E. Nováková

E. Nováková

  • Comenius University in Bratislava, Faculty of Natural Sciences, Department of Microbiology and VirologyBratislava, Slovak Republic
  • Comenius University in Bratislava, Faculty of Pharmacy, Department of Galenic PharmacyBratislava 3, Slovak Republic
  • Biomedical Research Center of the Slovak Academy of Sciences, Slovakia Institute of VirologyBratislava, Slovak Republic
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Nováková, E.
, 
V. Mikušová

V. Mikušová

Comenius University in Bratislava, Faculty of Pharmacy, Department of Galenic PharmacyBratislava 3, Slovak Republic
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Mikušová, V.
 e 
M. Šupolíková

M. Šupolíková

  • Comenius University in Bratislava, Faculty of Natural Sciences, Department of Microbiology and VirologyBratislava, Slovak Republic
  • Comenius University in Bratislava, Faculty of Pharmacy, Department of Galenic PharmacyBratislava 3, Slovak Republic
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Šupolíková, M.
  
24 ago 2023
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Categoria dell'articolo: Special Issue Article
Pubblicato online: 24 ago 2023
Pagine: 37 - 43
Ricevuto: 22 giu 2023
Accettato: 23 giu 2023
DOI: https://doi.org/10.2478/afpuc-2023-0008
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© 2023 E. Nováková et al., published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Figure 1.

Morphology of parental adherent monolayer and tumor sphere HeLa cells. (A) Parental HeLa cells cultured in Dulbecco’s modified Eagle’s medium + 10% fetal bovine serum grew as an adherent monolayer. (B) Parental HeLa cells cultured under a nonadhesive culture system formed typical tumor spheres (Wang et al., 2014).
Morphology of parental adherent monolayer and tumor sphere HeLa cells. (A) Parental HeLa cells cultured in Dulbecco’s modified Eagle’s medium + 10% fetal bovine serum grew as an adherent monolayer. (B) Parental HeLa cells cultured under a nonadhesive culture system formed typical tumor spheres (Wang et al., 2014).

Figure 2.

Adherent cell lines forming cell monolayers and spheroid structures (own resource).
Adherent cell lines forming cell monolayers and spheroid structures (own resource).

Figure 3.

Schematic diagrams of traditional two-dimensional (2D) monolayer cell culture (A) and three typical three-dimensional (3D) cell culture systems: cell spheroids/aggregates grown on a matrix (B), cells embedded in a matrix (C), and cell spheroids without a scaffold in suspension (D) (Edmondson et al., 2014 – modified).
Schematic diagrams of traditional two-dimensional (2D) monolayer cell culture (A) and three typical three-dimensional (3D) cell culture systems: cell spheroids/aggregates grown on a matrix (B), cells embedded in a matrix (C), and cell spheroids without a scaffold in suspension (D) (Edmondson et al., 2014 – modified).

Figure 4.

Schematic diagram of typical zones of cell proliferation in a 3D spheroid with models of oxygenation, nutrition, and CO2 removal (Edmondson et al., 2014 – modified).
Schematic diagram of typical zones of cell proliferation in a 3D spheroid with models of oxygenation, nutrition, and CO2 removal (Edmondson et al., 2014 – modified).

Figure 5.

Preparation of spheroids from an adherent cell culture, which serves to investigate the dynamics of autoadhesion and the kinetics of cell aggregation. It involves their self-assembly under nonadherent culture conditions, where cells are forced to aggregate on adhesion-restricting surfaces that are coated with agarose (own resource).
Preparation of spheroids from an adherent cell culture, which serves to investigate the dynamics of autoadhesion and the kinetics of cell aggregation. It involves their self-assembly under nonadherent culture conditions, where cells are forced to aggregate on adhesion-restricting surfaces that are coated with agarose (own resource).
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