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Spheroids as 3D Cell Models for Testing of Drugs

E. Nováková
E. Nováková
, 
V. Mikušová
V. Mikušová
 und 
M. Šupolíková
M. Šupolíková
   | 24. Aug. 2023
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Article Category: Special Issue Article
Online veröffentlicht: 24. Aug. 2023
Seitenbereich: 37 - 43
Eingereicht: 22. Juni 2023
Akzeptiert: 23. Juni 2023
DOI: https://doi.org/10.2478/afpuc-2023-0008
Schlüsselwörter
© 2023 E. Nováková et al., published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Figure 1.

Morphology of parental adherent monolayer and tumor sphere HeLa cells. (A) Parental HeLa cells cultured in Dulbecco’s modified Eagle’s medium + 10% fetal bovine serum grew as an adherent monolayer. (B) Parental HeLa cells cultured under a nonadhesive culture system formed typical tumor spheres (Wang et al., 2014).
Morphology of parental adherent monolayer and tumor sphere HeLa cells. (A) Parental HeLa cells cultured in Dulbecco’s modified Eagle’s medium + 10% fetal bovine serum grew as an adherent monolayer. (B) Parental HeLa cells cultured under a nonadhesive culture system formed typical tumor spheres (Wang et al., 2014).

Figure 2.

Adherent cell lines forming cell monolayers and spheroid structures (own resource).
Adherent cell lines forming cell monolayers and spheroid structures (own resource).

Figure 3.

Schematic diagrams of traditional two-dimensional (2D) monolayer cell culture (A) and three typical three-dimensional (3D) cell culture systems: cell spheroids/aggregates grown on a matrix (B), cells embedded in a matrix (C), and cell spheroids without a scaffold in suspension (D) (Edmondson et al., 2014 – modified).
Schematic diagrams of traditional two-dimensional (2D) monolayer cell culture (A) and three typical three-dimensional (3D) cell culture systems: cell spheroids/aggregates grown on a matrix (B), cells embedded in a matrix (C), and cell spheroids without a scaffold in suspension (D) (Edmondson et al., 2014 – modified).

Figure 4.

Schematic diagram of typical zones of cell proliferation in a 3D spheroid with models of oxygenation, nutrition, and CO2 removal (Edmondson et al., 2014 – modified).
Schematic diagram of typical zones of cell proliferation in a 3D spheroid with models of oxygenation, nutrition, and CO2 removal (Edmondson et al., 2014 – modified).

Figure 5.

Preparation of spheroids from an adherent cell culture, which serves to investigate the dynamics of autoadhesion and the kinetics of cell aggregation. It involves their self-assembly under nonadherent culture conditions, where cells are forced to aggregate on adhesion-restricting surfaces that are coated with agarose (own resource).
Preparation of spheroids from an adherent cell culture, which serves to investigate the dynamics of autoadhesion and the kinetics of cell aggregation. It involves their self-assembly under nonadherent culture conditions, where cells are forced to aggregate on adhesion-restricting surfaces that are coated with agarose (own resource).
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