Antibiotic, soothing and healing properties of panthenol are exploited in various pharmaceutical and cosmetic products. Only D-panthenol is biologically active while its L-form might counteract the biological effectiveness of the D-enantiomer. Hydrating and moisturizing effects are exhibited by both enantiomers. Therefore, it is necessary to develop a rapid and cheap method for the determination of panthenol enantiomeric excess in pharmaceutical preparations. In this study,