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New gene markers involved in regulation of granulosa cells development and differentiation towards endodermal and epithelial tissues – a new insight into the stemness specificity of ovarian follicular cells

Wiesława Kranc
Wiesława Kranc
, 
Małgorzata Popis
Małgorzata Popis
, 
Claudia Dompe
Claudia Dompe
, 
Afsaneh Golkar-Narenji
Afsaneh Golkar-Narenji
, 
Michal Jeseta
Michal Jeseta
, 
Paul E Mozdziak
Paul E Mozdziak
, 
Elena Kistanova
Elena Kistanova
, 
Alexander Makarevich
Alexander Makarevich
, 
Marie Machatkova
Marie Machatkova
, 
Dorota Bukowska
Dorota Bukowska
, 
Radek Prochazka
Radek Prochazka
, 
Jędrzej M. Jaśkowski
Jędrzej M. Jaśkowski
, 
Kornel Ratajczak
Kornel Ratajczak
, 
Jarosław Sobolewski
Jarosław Sobolewski
 e 
Paweł Antosik
Paweł Antosik
   | 30 dic 2021
Medical Journal of Cell Biology's Cover Image
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Pubblicato online: 30 dic 2021
Pagine: 177 - 187
Ricevuto: 21 ott 2021
Accettato: 08 dic 2021
DOI: https://doi.org/10.2478/acb-2021-0025
Parole chiave
© 2021 Wiesława Kranc et al., published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

FIGURE 1

Heatmaps presenting differentially expressed genes involved in “developmental process”, “cellular developmental process”, “regulation of cell differentiation”, “epithelium migration”, “epithelial cell proliferation”, “epithelial cell migration”, “epithelial cell differentiation”, “epithelial cell development”, “endodermal cell differentiation”, “endoderm formation”, “endoderm development” and “epithelium development” based on GO BP terms. Each row on the Y axis represents a single transcript. The red color indicates downregulated genes while the green are upregulated
Heatmaps presenting differentially expressed genes involved in “developmental process”, “cellular developmental process”, “regulation of cell differentiation”, “epithelium migration”, “epithelial cell proliferation”, “epithelial cell migration”, “epithelial cell differentiation”, “epithelial cell development”, “endodermal cell differentiation”, “endoderm formation”, “endoderm development” and “epithelium development” based on GO BP terms. Each row on the Y axis represents a single transcript. The red color indicates downregulated genes while the green are upregulated

FIGURE 2

The circular scatter plots of differentially expressed genes involved in “developmental process”, “cellular developmental process”, “regulation of cell differentiation”, “epithelium migration”, “epithelial cell proliferation”, “epithelial cell migration”, “epithelial cell differentiation”, “epithelial cell development”, “endodermal cell differentiation”, “endoderm formation”, “endoderm development” and “epithelium development” GO BP terms. Each dot represents a single gene. The z-scores were presented as segments of inner circles
The circular scatter plots of differentially expressed genes involved in “developmental process”, “cellular developmental process”, “regulation of cell differentiation”, “epithelium migration”, “epithelial cell proliferation”, “epithelial cell migration”, “epithelial cell differentiation”, “epithelial cell development”, “endodermal cell differentiation”, “endoderm formation”, “endoderm development” and “epithelium development” GO BP terms. Each dot represents a single gene. The z-scores were presented as segments of inner circles

FIGURE 3

The dendrogram of differentially expressed genes involved in “developmental process”, “cellular developmental process”, “regulation of cell differentiation”, “epithelium migration”, “epithelial cell proliferation”, “epithelial cell migration”, “epithelial cell differentiation”, “epithelial cell development”, “endodermal cell differentiation”, “endoderm formation”, “endoderm development” and “epithelium development” GO BP terms. The DEGs were clustered based on their logFC values
The dendrogram of differentially expressed genes involved in “developmental process”, “cellular developmental process”, “regulation of cell differentiation”, “epithelium migration”, “epithelial cell proliferation”, “epithelial cell migration”, “epithelial cell differentiation”, “epithelial cell development”, “endodermal cell differentiation”, “endoderm formation”, “endoderm development” and “epithelium development” GO BP terms. The DEGs were clustered based on their logFC values

FIGURE 4

Analysis of enriched gene ontological groups involved in regulation of granulosa cells differentiation towards endodermal and epithelial tissues. The network plot presenting the linkages of genes and GO BP terms
Analysis of enriched gene ontological groups involved in regulation of granulosa cells differentiation towards endodermal and epithelial tissues. The network plot presenting the linkages of genes and GO BP terms

FIGURE 5

Heatmap presenting the relationship between genes and selected GO BP terms. The yellow color of tiles indicates the absence of logFC values
Heatmap presenting the relationship between genes and selected GO BP terms. The yellow color of tiles indicates the absence of logFC values

FIGURE 6

Reactome FI network for “positive regulation of cell proliferation”. “--->” indicates activating/catalyzing, “-|” for inhibition, “-” FIs extracted from complexes or inputs and “---” predicted FIs
Reactome FI network for “positive regulation of cell proliferation”. “--->” indicates activating/catalyzing, “-|” for inhibition, “-” FIs extracted from complexes or inputs and “---” predicted FIs

FIGURE 7

Microarray validation using RT-qPCR
Microarray validation using RT-qPCR

The 6 most downregulated genes involved in regulation of granulosa cells differentiation towards endodermal and epithelial tissues were validated using RT-qPCR, 168h/0h

GENE NAME GENE SYMBOL FOLD CHANGE 48H/0H ADJ. P. VAL.
integral membrane protein 2A ITM2A -8.85 <0.05
disabled homolog 1 (Drosophila) DAB1 -8.91 <0.05
mal, T-cell differentiation protein 2 MAL2 -21.75 <0.05
nebulette NEBL -26.72 <0.05
death associated protein-like 1 DAPL1 -28.69 <0.05
hydroxysteroid (17-beta) dehydrogenase 1 HSD17B1 -35.75 <0.05

Primer sequences (5′-3′)

GENE NAME GENE SYMBOL PRIMER SEQUENCES (5′-3′)
integral membrane protein 2A ITM2A TCTCGTAGGCCTTTCCTTCAAGGCAGGAAGTAGGGCTCTC
disabled homolog 1 (Drosophila) DAB1 TACGTTTGTGGGAAGGAAGGCTTCCTTCTTTTGGCTGGTG
mal, T-cell differentiation protein 2 MAL2 AGGATGGGTCATGTTCGTGTTTGTCATTCAAGAGCGGCTG
nebulette NEBL CAAACCCTTCAAGGCTACCACTGAGAACACGCTTCCATCA
death associated protein-like 1 DAPL1 CCTGCTCTGGAGAAGGTCACGGGCCTAAGGAAAGTTTTGG
hydroxysteroid (17-beta) dehydrogenase 1 HSD17B1 GTGTCAGAGGCTTGCTAGGGCAGCACAATCTCAAGGCTGA
eISSN:
2544-3577
Lingua:
Inglese
Frequenza di pubblicazione:
4 volte all'anno
Argomenti della rivista:
Life Sciences, Molecular Biology, Biochemistry
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