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New gene markers involved in regulation of granulosa cells development and differentiation towards endodermal and epithelial tissues – a new insight into the stemness specificity of ovarian follicular cells

Wiesława Kranc

Wiesława Kranc

Department of Anatomy, Poznań University of Medical SciencesPoznań, Poland
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Kranc, Wiesława
, 
Małgorzata Popis

Małgorzata Popis

Department of Anatomy, Poznań University of Medical SciencesPoznań, Poland
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Popis, Małgorzata
, 
Claudia Dompe

Claudia Dompe

Department of Anatomy, Poznań University of Medical SciencesPoznań, Poland
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Dompe, Claudia
, 
Afsaneh Golkar-Narenji

Afsaneh Golkar-Narenji

Prestage Department of Poultry Science, North Carolina State UniversityRaleigh, USA
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Golkar-Narenji, Afsaneh
, 
Michal Jeseta

Michal Jeseta

  • Department of Obstetrics and Gynecology, University Hospital and Masaryk UniversityBrno, Czech Republic
  • Department of Veterinary Sciences, Czech University of Life Sciences in PraguePrague, Czech Republic
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Jeseta, Michal
, 
Paul E Mozdziak

Paul E Mozdziak

  • Prestage Department of Poultry Science, North Carolina State UniversityRaleigh, USA
  • Physiology Graduate Program, North Carolina State UniversityRaleigh, USA
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Mozdziak, Paul E
, 
Elena Kistanova

Elena Kistanova

Institute of Biology and Immunology of Reproduction, Bulgarian Academy of SciencesSofia, Bulgaria
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Kistanova, Elena
, 
Alexander Makarevich

Alexander Makarevich

National Agricultural and Food Centre, Research Institute of Animal Production NitraNitra, Slovak Republic
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Makarevich, Alexander
, 
Marie Machatkova

Marie Machatkova

Veterinary Research InstituteBrno, Czech Republic
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Machatkova, Marie
, 
Dorota Bukowska

Dorota Bukowska

Department of Diagnostics and Clinical Sciences, Institute of Veterinary Medicine, Nicolaus Copernicus University in ToruńToruń, Poland
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Bukowska, Dorota
, 
Radek Prochazka

Radek Prochazka

Laboratory of Developmental Biology, Institute of Animal Physiology and Genetics of the Czech Academy of SciencesLiběchov, Czech Republic
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Prochazka, Radek
, 
Jędrzej M. Jaśkowski

Jędrzej M. Jaśkowski

Department of Diagnostics and Clinical Sciences, Institute of Veterinary Medicine, Nicolaus Copernicus University in ToruńToruń, Poland
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Jaśkowski, Jędrzej M.
, 
Kornel Ratajczak

Kornel Ratajczak

Department of Veterinary Surgery, Institute of Veterinary Medicine, Nicolaus Copernicus University in ToruńToruń, Poland
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Ratajczak, Kornel
, 
Jarosław Sobolewski

Jarosław Sobolewski

Department of Public Health Protection and Animal Welfare, Institute of Veterinary Medicine, Nicolaus Copernicus University in ToruńToruń,
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Sobolewski, Jarosław
 et 
Paweł Antosik

Paweł Antosik

Department of Veterinary Surgery, Institute of Veterinary Medicine, Nicolaus Copernicus University in ToruńToruń, Poland
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Antosik, Paweł
  
30 déc. 2021
New gene markers involved in regulation of granulosa cells development and differentiation towards endodermal and epithelial tissues – a new insight into the stemness specificity of ovarian follicular cells's Cover Image
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Publié en ligne: 30 déc. 2021
Pages: 177 - 187
Reçu: 21 oct. 2021
Accepté: 08 déc. 2021
DOI: https://doi.org/10.2478/acb-2021-0025
Mots clés
© 2021 Wiesława Kranc et al., published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

FIGURE 1

Heatmaps presenting differentially expressed genes involved in “developmental process”, “cellular developmental process”, “regulation of cell differentiation”, “epithelium migration”, “epithelial cell proliferation”, “epithelial cell migration”, “epithelial cell differentiation”, “epithelial cell development”, “endodermal cell differentiation”, “endoderm formation”, “endoderm development” and “epithelium development” based on GO BP terms. Each row on the Y axis represents a single transcript. The red color indicates downregulated genes while the green are upregulated
Heatmaps presenting differentially expressed genes involved in “developmental process”, “cellular developmental process”, “regulation of cell differentiation”, “epithelium migration”, “epithelial cell proliferation”, “epithelial cell migration”, “epithelial cell differentiation”, “epithelial cell development”, “endodermal cell differentiation”, “endoderm formation”, “endoderm development” and “epithelium development” based on GO BP terms. Each row on the Y axis represents a single transcript. The red color indicates downregulated genes while the green are upregulated

FIGURE 2

The circular scatter plots of differentially expressed genes involved in “developmental process”, “cellular developmental process”, “regulation of cell differentiation”, “epithelium migration”, “epithelial cell proliferation”, “epithelial cell migration”, “epithelial cell differentiation”, “epithelial cell development”, “endodermal cell differentiation”, “endoderm formation”, “endoderm development” and “epithelium development” GO BP terms. Each dot represents a single gene. The z-scores were presented as segments of inner circles
The circular scatter plots of differentially expressed genes involved in “developmental process”, “cellular developmental process”, “regulation of cell differentiation”, “epithelium migration”, “epithelial cell proliferation”, “epithelial cell migration”, “epithelial cell differentiation”, “epithelial cell development”, “endodermal cell differentiation”, “endoderm formation”, “endoderm development” and “epithelium development” GO BP terms. Each dot represents a single gene. The z-scores were presented as segments of inner circles

FIGURE 3

The dendrogram of differentially expressed genes involved in “developmental process”, “cellular developmental process”, “regulation of cell differentiation”, “epithelium migration”, “epithelial cell proliferation”, “epithelial cell migration”, “epithelial cell differentiation”, “epithelial cell development”, “endodermal cell differentiation”, “endoderm formation”, “endoderm development” and “epithelium development” GO BP terms. The DEGs were clustered based on their logFC values
The dendrogram of differentially expressed genes involved in “developmental process”, “cellular developmental process”, “regulation of cell differentiation”, “epithelium migration”, “epithelial cell proliferation”, “epithelial cell migration”, “epithelial cell differentiation”, “epithelial cell development”, “endodermal cell differentiation”, “endoderm formation”, “endoderm development” and “epithelium development” GO BP terms. The DEGs were clustered based on their logFC values

FIGURE 4

Analysis of enriched gene ontological groups involved in regulation of granulosa cells differentiation towards endodermal and epithelial tissues. The network plot presenting the linkages of genes and GO BP terms
Analysis of enriched gene ontological groups involved in regulation of granulosa cells differentiation towards endodermal and epithelial tissues. The network plot presenting the linkages of genes and GO BP terms

FIGURE 5

Heatmap presenting the relationship between genes and selected GO BP terms. The yellow color of tiles indicates the absence of logFC values
Heatmap presenting the relationship between genes and selected GO BP terms. The yellow color of tiles indicates the absence of logFC values

FIGURE 6

Reactome FI network for “positive regulation of cell proliferation”. “--->” indicates activating/catalyzing, “-|” for inhibition, “-” FIs extracted from complexes or inputs and “---” predicted FIs
Reactome FI network for “positive regulation of cell proliferation”. “--->” indicates activating/catalyzing, “-|” for inhibition, “-” FIs extracted from complexes or inputs and “---” predicted FIs

FIGURE 7

Microarray validation using RT-qPCR
Microarray validation using RT-qPCR

The 6 most downregulated genes involved in regulation of granulosa cells differentiation towards endodermal and epithelial tissues were validated using RT-qPCR, 168h/0h

GENE NAME GENE SYMBOL FOLD CHANGE 48H/0H ADJ. P. VAL.
integral membrane protein 2A ITM2A -8.85 <0.05
disabled homolog 1 (Drosophila) DAB1 -8.91 <0.05
mal, T-cell differentiation protein 2 MAL2 -21.75 <0.05
nebulette NEBL -26.72 <0.05
death associated protein-like 1 DAPL1 -28.69 <0.05
hydroxysteroid (17-beta) dehydrogenase 1 HSD17B1 -35.75 <0.05

Primer sequences (5′-3′)

GENE NAME GENE SYMBOL PRIMER SEQUENCES (5′-3′)
integral membrane protein 2A ITM2A TCTCGTAGGCCTTTCCTTCAAGGCAGGAAGTAGGGCTCTC
disabled homolog 1 (Drosophila) DAB1 TACGTTTGTGGGAAGGAAGGCTTCCTTCTTTTGGCTGGTG
mal, T-cell differentiation protein 2 MAL2 AGGATGGGTCATGTTCGTGTTTGTCATTCAAGAGCGGCTG
nebulette NEBL CAAACCCTTCAAGGCTACCACTGAGAACACGCTTCCATCA
death associated protein-like 1 DAPL1 CCTGCTCTGGAGAAGGTCACGGGCCTAAGGAAAGTTTTGG
hydroxysteroid (17-beta) dehydrogenase 1 HSD17B1 GTGTCAGAGGCTTGCTAGGGCAGCACAATCTCAAGGCTGA
Langue:
Anglais
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Sujets de la revue:
Sciences de la vie, Biologie moléculaire, Biochimie
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