<abstract xmlns="http://www.w3.org/1999/xhtml"><p>Ovarian granulosa cells (GCs) play a crucial role in oocyte maturation, creating a favorable microenvironment around the oocyte. Therefore, enhanced apoptosis and GCs loss may negatively affect the intra-follicular milieu and compromise the oocyte quality, reducing pregnancy chances. Based on the RT-qPCR method, the present research revealed the differential expression of apoptosis-related genes (<italic>BCL2</italic>, <italic>BAX</italic>, <italic>p53</italic>, <italic>CASP9</italic>) during the seven days of primary <italic>in vitro</italic> culture of GCs isolated from patients undergoing <italic>in vitro</italic> fertilization (IVF) procedure. Individual gene expression changes may reflect the GCs survival and/or apoptotic status at different time points.</p><p><bold>Running title</bold>: Apoptosis-related genes expression in granulosa cells <italic>in vitro</italic></p></abstract>

Ovarian granulosa cells (GCs) play a crucial role in oocyte maturation, creating a favorable microenvironment around the oocyte. Therefore, enhanced apoptosis and GCs loss may negatively affect the intra-follicular milieu and compromise the oocyte quality, reducing pregnancy chances. Based on the RT-qPCR method, the present research revealed the differential expression of apoptosis-related genes (BCL2, BAX, p53, CASP9) during the seven days of primary in vitro culture of GCs isolated from patients undergoing in vitro fertilization (IVF) procedure. Individual gene expression changes may reflect the GCs survival and/or apoptotic status at different time points.Running title: Apoptosis-related genes expression in granulosa cells in vitro

Apoptosis-related genes expression in primary in vitro culture of human ovarian granulosa cells

Medical Journal of Cell Biology

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Ovarian granulosa cells (GCs) play a crucial role in oocyte maturation, creating a favorable microenvironment around the oocyte. Therefore, enhanced apoptosis...

Apoptosis-related genes expression in primary in vitro culture of human ovarian granulosa cells

Pubblicato online: 31 dic 2020

Pagine: 176 - 182

Ricevuto: 04 nov 2020

Accettato: 15 dic 2020

DOI: https://doi.org/10.2478/acb-2020-0023

Parole chiave
apoptosis-related genes, granulosa cells, primary culture

© 2020 Ievgeniia Kocherova, Katarzyna Stefańska, Rut Bryl, Joanna Perek, Wojciech Pieńkowski, Jana Zakova, Igor Crha, Pavel Ventruba, Paul Mozdziak, Michal Ješeta, published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Apoptosis-related genes expression in primary in vitro culture of human ovarian granulosa cells

Pubblicato online: 31 dic 2020

Pagine: 176 - 182

Ricevuto: 04 nov 2020

Accettato: 15 dic 2020

DOI: https://doi.org/10.2478/acb-2020-0023

Parole chiaveapoptosis-related genes, granulosa cells, primary culture

© 2020 Ievgeniia Kocherova, Katarzyna Stefańska, Rut Bryl, Joanna Perek, Wojciech Pieńkowski, Jana Zakova, Igor Crha, Pavel Ventruba, Paul Mozdziak, Michal Ješeta, published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Parole chiave
apoptosis-related genes, granulosa cells, primary culture