Enzyme | Hydrolyzed bounds | Number of enzymatic units added per 100 μl of reaction |
---|---|---|
Catalyzes the removal of core 1 and core 3 | 400000 U | |
PNGase F | Cleaves between the innermost GlcNAc and asparagine residues of high mannose, hybrid, and complex oligosaccharides from | 5000 U |
Neuraminidase | Catalyzes the hydrolysis of terminal, non-reducing α2,3, α2,6, and α2,8 linked | 500 U |
β1-4 Galactosidase | Catalyzes the hydrolysis of terminal, non-reducing β1-4 linked D-galactopyranosyl residues from oligosaccharides and glycoproteins. | 80 U |
β- | Catalyzes the hydrolysis of terminal, non-reducing β- | 40 U |
GT inhibitor | The enzyme inhibited |
---|---|
Castanospermine | β-glucosidase |
Deoxymannojirimycin | α-(1→2)-mannosidase |
Kifunensine | |
Swainsonine | α-(1→3)-mannosidase |
Deoxynojirimycin hydrochloride | α-glucosidase |
Monomer | % of a monomer in EPS isolated from the strain tested | |
---|---|---|
Maltose | 0.71 | 0 |
Glucose | 30.9 | 15.7 |
Galactose | 53.7 | 31.4 |
Arabinose | 14.6 | 51.6 |
Ribose | 0 | 1.4 |