Allele Sizes (bp) | |||||
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RKNP17a | RKNP135 | RKNP342 | RKNP402 | RKNP529 | |
NC_012872 (bp)b | (52,744,750) | (53,190,542) | (53,974,058) | (54,199,591) | (54,678,311) |
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Honey Drip |
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Entry 22 | 198, 198 | 210, 210 | 222, 222 | 204, 204 | 185, 185 |
Entry 22-BC1F6 |
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Collier | 198, 198 | 200, 200 | 220, 220 | 253, 253 | 116, 116 |
Collier-BC1F6 |
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GT-IR7 | 198, 245 | 210, 210 | 220, 220 | 265, 265 | 116, 232 |
GT-IR7-BC1F6 |
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Dale | 198, 198 | 206, 206 | 222, 222 | 234, 234 | 185, 185 |
Dale-BC1F6 |
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Topper | 198, 198 | 202, 202 | 220, 220 | 255, 255 | 116, 232 |
Top 76-6-BC1F6 |
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Trial 1 | Trial 2 | |||
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Genotype | Root weight (g)a | Root weight (g)a | ||
Top 76-6 | 63.3 | Ab | 97.8 | Ab |
Top 76-6-BC1F6 | 56.6 | AB | 69.2 | B |
Honey Drip | 43.9 | BC | 52.6 | BCD |
Dale-BC1F6 | 41.4 | BCD | 53.7 | BC |
Entry 22-BC1F6 | 41.1 | CD | 43.3 | CDE |
Collier | 40.9 | CD | 32.9 | DE |
Collier-BC1F6 | 40.1 | CD | 31.5 | E |
GT-IR7 | 38.5 | CD | 27.9 | E |
GT-IR7-BC1F6 | 38.3 | CD | 30.6 | E |
Dale | 37.1 | CD | 39.5 | CDE |
Entry 22 | 26.6 | D | 24.0 | E |
Genotype | Total eggsa | Eggs/g roota | ||
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Dale | 425775 | Ab | 11141 | Ab |
Top 76-6 | 297250 | A | 3977 | A |
Collier | 206325 | A | 5856 | A |
GT-IR7 | 170125 | A | 5584 | A |
Entry 22 | 115808 | A | 4661 | A |
Honey Drip | 2300 | B | 48 | B |
Collier-BC1F6 | 1175 | B | 36 | B |
Entry 22-BC1F6 | 975 | B | 20 | B |
Dale-BC1F6 | 875 | B | 19 | B |
Top 76-6-BC1F6 | 750 | B | 18 | B |
GT-IR7-BC1F6 | 625 | B | 16 | B |
Date of PCR | Sorghum seedling(s) with confirmed cross | Microsatelite markers used |
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7/17/2015 | Honey Drip x Collier | TRKN1, TRKN3, TRKN4 |
8/4/2015 | Honey Drip x GT-IR7 | TRKN1, TRKN3, TRKN4, TRKN5 |
8/4/2015 | Honey Drip x Entry 22 | TRKN1, TRKN3, TRKN4, TRKN5 |
8/4/2015 | Honey Drip x Entry 22 | TRKN1, TRKN3, TRKN4, TRKN5 |
8/25/2015 | Honey Drip x Dale | TRKN1, TRKN3, TRKN4, TRKN5 |
11/30/2015 | Honey Drip x Top 76-6 | RKNP402, RKNP342 |
11/30/2015 | Entry 22 x (Honey Drip x Entry 22) | RKNP402, RKNP342 |
12/16/2015 | Honey Drip x Top 76-6 | RKNP194, RKNP259, RKNP402, RKNP529 |
1/26/2016 | Dale x (Honey Drip x Dale) | RKNP194, RKNP259, RKNP465, RKNP529 |
2/19/2016 | GT-IR7 x (Honey Drip x GT-IR7) | TRKN4, TRKN3, RKNP529, RKNP638 |
2/29/2016 | Collier x (Honey Drip x Collier) | RKNP529, RKNP638 |
3/9/2016 | GT-IR7 x (Honey Drip x GT-IR7) | RKNP529, TRKN3, RKNP638 |
3/14/2016 | GT-IR7 x (Honey Drip x GT-IR7) | RKNP638, RKNP194, RKNP465 |
3/15/2016 | GT-IR7 x (Honey Drip x GT-IR7) | RKNP638, RKNP194, RKNP465 |
3/28/2016 | Entry 22 x (Honey Drip x Entry 22) F2 homozygous | RKNP342, RKNP402, RKNP529 |
3/28/2016 | Collier x (Honey Drip x Collier) | RKNP342, RKNP402, RKNP529 |
4/20/2016 | Top 76-6 x (Honey Drip x Top 76-6) | RKNP342, RKNP402, RKNP529 |
4/20/2016 | Entry 22 x (Honey Drip x Entry 22) F2 homozygous | RKNP342, RKNP402, RKNP529 |
4/21/2016 | Entry 22 x (Honey Drip x Entry 22) F2 homozygous | RKNP342, RKNP402, RKNP529 |
5/6/2016 | Dale x (Honey Drip x Dale) F2 homozygous | RKNP342, RKNP402, RKNP529 |
5/11/2016 | Dale x (Honey Drip x Dale) F2 homozygous | RKNP342, RKNP402, RKNP529 |
6/1/2016 | Dale x (Honey Drip x Dale) F2 homozygous | RKNP342, RKNP402, RKNP529 |
6/8/2016 | Dale x (Honey Drip x Dale) F2 homozygous | RKNP342, RKNP402, RKNP529 |
6/9/2016 | GT-IR7 x (Honey Drip x GT-IR7) F2 homozygous | RKNP342, RKNP402, RKNP529 |
6/22/2016 | Collier x (Honey Drip x Collier) F2 homozygous | RKNP342, RKNP402, RKNP529 |
8/30/2016 | Top76-6 x (Honey Drip x Top 76-6) F2 homozygous | RKNP529, RKNP638, RKNP709, RKNP821 |
6/15/2017 | Genotyping of BC1F6 lines to determine the size of the Honey Drip crossover in the RKN region | RKNP17, RKNP135, RKNP342, RKNP402, RKNP529 |
Name | PI number | Reference/source | Sorghum type |
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Collier | PI 641862 |
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sweet |
Dale | PI 651495 |
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sweet |
Entry 22 | – | University of Florida | forage |
GT-IR7 | PI 602445 |
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grain |
Top 76-6 | PI 583832 |
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sweet |
Marker | Forward sequence | Reverse sequence | Repeat motif | Expected amplicon (bp)a |
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RKNP17 | GCAGTTTTTCAAGGAACGTG | GAGGAATGGGTGATGAAACAA | (TTA)106 | 422 |
RKNP135 | GTTTCGTTTCAATCGGCTTC | GCGCCCCATCATATGTCTT | (AAG)21 | 197 |
RKNP194 | TCATACTACCACAGCCGCTAGA | TGGTGTAGATGTGTGTGATTCAA | (AT)40 | 236 |
RKNP259 | AGCTCTTCAGGCACATCGTT | TCTCCTTCCCACCCTGTATG | (GA)48 | 243 |
RKNP342 | TTCCAACAGGCAAACAACAG | TCATGGCCTGTGTATCAAGC | (CT)25 | 205 |
RKNP402 | TCAGCAAGATGGTTGGTTGA | ACGAGGCCGTTGAGATTATG | (TTA)22 | 213 |
RKNP465 | TGACTGAGAGGGTCTACCTAACG | CAACCGGAAGTACGCTGATT | (AT)19 | 247 |
RKNP529 | GCGAAATGGAGAAGAACAGG | CGTCATCAGCTTCCAGGAGT | (GA)25 | 199 |
RKNP638 | CCACACCGGTTTCCTGTTAT | TAATAAGCCCCGCATGAAGA | (ATA)21, (TAT)6, (TAA)25 | 251 |
RKNP709 | GCAAGCTGAAGTGGCCTAGT | CTACTCCCTCCGTCCCAAAT | (CT)9, (TA)18 | 324 |
RKNP821 | CTCGGCAGCACCAAATAAAA | TCTCAACCGATGATTGTCCA | (TA)51 | 199 |
TRKN1 | TGTACACTGCATGCCAACCT | GCCTCGTCTGGTTCATTGTT | (AT)14 | 246 |
TRKN3 | GAAGAATTGCTCCAGGAACG | AAGCAGTATCCGGGGAAGAT | (TA)10 | 271 |
TRKN4 | CGTAAATGGAGGTGGCTACA | CCCGGTTGGTACAACATAGA | (AT)29 | 232 |
TRKN5 | ACTGTTATGTCGGCTGGTCA | AGTGTTACTGCCTGGCCAAA | (TC)11 | 196 |