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Transfer of Meloidogyne incognita Resistance Using Marker-assisted Selection in Sorghum

Richard F. Davis
Richard F. Davis
, 
Karen R. Harris-Shultz
Karen R. Harris-Shultz
, 
Joseph E. Knoll
Joseph E. Knoll
 und 
Hongliang Wang
Hongliang Wang
   | 11. Nov. 2021
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Online veröffentlicht: 11. Nov. 2021
Seitenbereich: 1 - 10
DOI: https://doi.org/10.21307/jofnem-2021-087
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© 2021 Richard F. Davis et al., published by Sciendo.
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Figure 1:

Polyacrylamide gel image of microsatellite marker RKNP135 from the sorghum donor line ‘Honey Drip’, the recurrent parents ‘Collier’, GT-IR7, ‘Dale’, and ‘Top 76-6’, and the BC1F6 progeny lines. A 50-350 bp sizing standard (LI-COR Biosciences, Lincoln, NE) was loaded in the first and last lanes.
Polyacrylamide gel image of microsatellite marker RKNP135 from the sorghum donor line ‘Honey Drip’, the recurrent parents ‘Collier’, GT-IR7, ‘Dale’, and ‘Top 76-6’, and the BC1F6 progeny lines. A 50-350 bp sizing standard (LI-COR Biosciences, Lincoln, NE) was loaded in the first and last lanes.

Presence of the introgression from ‘Honey Drip’ in the QTL-Sb.RKN.3.1 region of each backcross (BC1F6) line.

Allele Sizes (bp)
RKNP17a RKNP135 RKNP342 RKNP402 RKNP529
NC_012872 (bp)b (52,744,750) (53,190,542) (53,974,058) (54,199,591) (54,678,311)
Genotype
 Honey Drip 120, 120 153, 153 240, 240 173, 173 222, 222
 Entry 22 198, 198 210, 210 222, 222 204, 204 185, 185
 Entry 22-BC1F6 120, 120 153, 153 240, 240 173, 173 222, 222
 Collier 198, 198 200, 200 220, 220 253, 253 116, 116
 Collier-BC1F6 120, 120 153, 153 240, 240 173, 173 222, 222
 GT-IR7 198, 245 210, 210 220, 220 265, 265 116, 232
 GT-IR7-BC1F6 120, 120 153, 153 240, 240 173, 173 222 , 232
 Dale 198, 198 206, 206 222, 222 234, 234 185, 185
 Dale-BC1F6 120, 120 153, 153 240, 240 173, 173 222, 222
 Topper 198, 198 202, 202 220, 220 255, 255 116, 232
 Top 76-6-BC1F6 120, 120 153, 153 240, 240 173, 173 222 , 232

Fresh root weights of parental sorghum genotypes and their BC1F6 progeny.

Trial 1 Trial 2
Genotype Root weight (g)a Root weight (g)a
Top 76-6 63.3 Ab 97.8 Ab
Top 76-6-BC1F6 56.6 AB 69.2 B
Honey Drip 43.9 BC 52.6 BCD
Dale-BC1F6 41.4 BCD 53.7 BC
Entry 22-BC1F6 41.1 CD 43.3 CDE
Collier 40.9 CD 32.9 DE
Collier-BC1F6 40.1 CD 31.5 E
GT-IR7 38.5 CD 27.9 E
GT-IR7-BC1F6 38.3 CD 30.6 E
Dale 37.1 CD 39.5 CDE
Entry 22 26.6 D 24.0 E

Total M. incognita eggs and eggs/gram of root of parental sorghum genotypes and their BC1F6 progeny that have the M. incognita resistance QTL QTL-Sb.RKN.3.1.

Genotype Total eggsa Eggs/g roota
Dale 425775 Ab 11141 Ab
Top 76-6 297250 A 3977 A
Collier 206325 A 5856 A
GT-IR7 170125 A 5584 A
Entry 22 115808 A 4661 A
Honey Drip 2300 B 48 B
Collier-BC1F6 1175 B 36 B
Entry 22-BC1F6 975 B 20 B
Dale-BC1F6 875 B 19 B
Top 76-6-BC1F6 750 B 18 B
GT-IR7-BC1F6 625 B 16 B

Genotyping information for the creation of sorghum BC1F6 lines.

Date of PCR Sorghum seedling(s) with confirmed cross Microsatelite markers used
7/17/2015 Honey Drip x Collier TRKN1, TRKN3, TRKN4
8/4/2015 Honey Drip x GT-IR7 TRKN1, TRKN3, TRKN4, TRKN5
8/4/2015 Honey Drip x Entry 22 TRKN1, TRKN3, TRKN4, TRKN5
8/4/2015 Honey Drip x Entry 22 TRKN1, TRKN3, TRKN4, TRKN5
8/25/2015 Honey Drip x Dale TRKN1, TRKN3, TRKN4, TRKN5
11/30/2015 Honey Drip x Top 76-6 RKNP402, RKNP342
11/30/2015 Entry 22 x (Honey Drip x Entry 22) RKNP402, RKNP342
12/16/2015 Honey Drip x Top 76-6 RKNP194, RKNP259, RKNP402, RKNP529
1/26/2016 Dale x (Honey Drip x Dale) RKNP194, RKNP259, RKNP465, RKNP529
2/19/2016 GT-IR7 x (Honey Drip x GT-IR7) TRKN4, TRKN3, RKNP529, RKNP638
2/29/2016 Collier x (Honey Drip x Collier) RKNP529, RKNP638
3/9/2016 GT-IR7 x (Honey Drip x GT-IR7) RKNP529, TRKN3, RKNP638
3/14/2016 GT-IR7 x (Honey Drip x GT-IR7) RKNP638, RKNP194, RKNP465
3/15/2016 GT-IR7 x (Honey Drip x GT-IR7) RKNP638, RKNP194, RKNP465
3/28/2016 Entry 22 x (Honey Drip x Entry 22) F2 homozygous RKNP342, RKNP402, RKNP529
3/28/2016 Collier x (Honey Drip x Collier) RKNP342, RKNP402, RKNP529
4/20/2016 Top 76-6 x (Honey Drip x Top 76-6) RKNP342, RKNP402, RKNP529
4/20/2016 Entry 22 x (Honey Drip x Entry 22) F2 homozygous RKNP342, RKNP402, RKNP529
4/21/2016 Entry 22 x (Honey Drip x Entry 22) F2 homozygous RKNP342, RKNP402, RKNP529
5/6/2016 Dale x (Honey Drip x Dale) F2 homozygous RKNP342, RKNP402, RKNP529
5/11/2016 Dale x (Honey Drip x Dale) F2 homozygous RKNP342, RKNP402, RKNP529
6/1/2016 Dale x (Honey Drip x Dale) F2 homozygous RKNP342, RKNP402, RKNP529
6/8/2016 Dale x (Honey Drip x Dale) F2 homozygous RKNP342, RKNP402, RKNP529
6/9/2016 GT-IR7 x (Honey Drip x GT-IR7) F2 homozygous RKNP342, RKNP402, RKNP529
6/22/2016 Collier x (Honey Drip x Collier) F2 homozygous RKNP342, RKNP402, RKNP529
8/30/2016 Top76-6 x (Honey Drip x Top 76-6) F2 homozygous RKNP529, RKNP638, RKNP709, RKNP821
6/15/2017 Genotyping of BC1F6 lines to determine the size of the Honey Drip crossover in the RKN region RKNP17, RKNP135, RKNP342, RKNP402, RKNP529

Recurrent sorghum parents used for marker-assisted selection of the M. incognita resistance QTL, QTL-Sb.RKN.3.1, from ‘Honey Drip’ (PI 641821).

Name PI number Reference/source Sorghum type
Collier PI 641862 Maunder (2000) sweet
Dale PI 651495 Broadhead and Coleman (1973) sweet
Entry 22 – University of Florida forage
GT-IR7 PI 602445 Widstrom (1998) grain
Top 76-6 PI 583832 Day et al. (1995) sweet

Sorghum primer sequences of microsatellite markers used for confirming sorghum crosses in the M. incognita resistance quantitative trait locus region.

Marker Forward sequence Reverse sequence Repeat motif Expected amplicon (bp)a
RKNP17 GCAGTTTTTCAAGGAACGTG GAGGAATGGGTGATGAAACAA (TTA)106 422
RKNP135 GTTTCGTTTCAATCGGCTTC GCGCCCCATCATATGTCTT (AAG)21 197
RKNP194 TCATACTACCACAGCCGCTAGA TGGTGTAGATGTGTGTGATTCAA (AT)40 236
RKNP259 AGCTCTTCAGGCACATCGTT TCTCCTTCCCACCCTGTATG (GA)48 243
RKNP342 TTCCAACAGGCAAACAACAG TCATGGCCTGTGTATCAAGC (CT)25 205
RKNP402 TCAGCAAGATGGTTGGTTGA ACGAGGCCGTTGAGATTATG (TTA)22 213
RKNP465 TGACTGAGAGGGTCTACCTAACG CAACCGGAAGTACGCTGATT (AT)19 247
RKNP529 GCGAAATGGAGAAGAACAGG CGTCATCAGCTTCCAGGAGT (GA)25 199
RKNP638 CCACACCGGTTTCCTGTTAT TAATAAGCCCCGCATGAAGA (ATA)21, (TAT)6, (TAA)25 251
RKNP709 GCAAGCTGAAGTGGCCTAGT CTACTCCCTCCGTCCCAAAT (CT)9, (TA)18 324
RKNP821 CTCGGCAGCACCAAATAAAA TCTCAACCGATGATTGTCCA (TA)51 199
TRKN1 TGTACACTGCATGCCAACCT GCCTCGTCTGGTTCATTGTT (AT)14 246
TRKN3 GAAGAATTGCTCCAGGAACG AAGCAGTATCCGGGGAAGAT (TA)10 271
TRKN4 CGTAAATGGAGGTGGCTACA CCCGGTTGGTACAACATAGA (AT)29 232
TRKN5 ACTGTTATGTCGGCTGGTCA AGTGTTACTGCCTGGCCAAA (TC)11 196
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