<abstract xmlns="http://www.w3.org/1999/xhtml"><p> A set of eight simple sequence repeat (SSR) markers were developed from 707 Salix expressed sequence tags (ESTs) deposited in GenBank. Each of the EST-SSR amplicons was identical to the original EST, with sequence identity 60.90-96.03% and presence of the expected repeat motifs. Of the eight EST-SSR loci, five were polymorphic among 14 individuals of S. eriocephala, with the number of alleles per locus (Na), observed heterozygosity (H<sub>o</sub>), expected heterozygosity (He) and polymorphic information content (PIC) being 2-7 (mean 4.8), 0.29-0.85 (mean 0.65), 0.25-0.84 (mean 0.65) and 0.21-0.78 (mean 0.58), respectively. High rates of crossspecies/ genus amplification were also observed within fourteen different species. The primer sequences for the eight EST-SSRs have been deposited in the Probe database of GenBank (IDs Pr031820546 - Pr031820553). The EST-SSRs developed herein would be a valuable addition of functional markers for genetics and breeding applications in a wide range of Salix species.</p></abstract>

A set of eight simple sequence repeat (SSR) markers were developed from 707 Salix expressed sequence tags (ESTs) deposited in GenBank. Each of the EST-SSR amplicons was identical to the original EST, with sequence identity 60.90-96.03% and presence of the expected repeat motifs. Of the eight EST-SSR loci, five were polymorphic among 14 individuals of S. eriocephala, with the number of alleles per locus (Na), observed heterozygosity (Ho), expected heterozygosity (He) and polymorphic information content (PIC) being 2-7 (mean 4.8), 0.29-0.85 (mean 0.65), 0.25-0.84 (mean 0.65) and 0.21-0.78 (mean 0.58), respectively. High rates of crossspecies/ genus amplification were also observed within fourteen different species. The primer sequences for the eight EST-SSRs have been deposited in the Probe database of GenBank (IDs Pr031820546 - Pr031820553). The EST-SSRs developed herein would be a valuable addition of functional markers for genetics and breeding applications in a wide range of Salix species.

Short note: Development, characterization and cross-amplification of eight EST-derived microsatellites in Salix

Silvae Genetica

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

A set of eight simple sequence repeat (SSR) markers were developed from 707 Salix expressed sequence tags (ESTs) deposited in GenBank. Each of the EST-SSR...

Short note: Development, characterization and cross-amplification of eight EST-derived microsatellites in Salix

Pubblicato online: 01 giu 2017

Pagine: 113 - 115

Ricevuto: 04 nov 2013

DOI: https://doi.org/10.1515/sg-2014-0015

Parole chiave
Salix, Willow, Expressed sequence tag (EST), Simple sequence repeat (SSR), Microsatellites

© 2017

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Short note: Development, characterization and cross-amplification of eight EST-derived microsatellites in Salix

Pubblicato online: 01 giu 2017

Pagine: 113 - 115

Ricevuto: 04 nov 2013

DOI: https://doi.org/10.1515/sg-2014-0015

Parole chiaveSalix, Willow, Expressed sequence tag (EST), Simple sequence repeat (SSR), Microsatellites

© 2017

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Parole chiave
Salix, Willow, Expressed sequence tag (EST), Simple sequence repeat (SSR), Microsatellites