<abstract xmlns="http://www.w3.org/1999/xhtml">The in-house production of fluorescently labelledinternal size standard offers the advantage of cost savingover the commercial size standard in microsatellitegenotyping. Based on the reported in-house internal sizestandard protocol, we have improved the method by generating21 DNA fragments (in a standard named as HM-400) with each size similar to that of the commercialsize standard. The consistent amplification of the correctfragment size was optimised via primer modulationfor non-templated nucleotide addition by Taq DNA polymerase.A total of six microsatellite loci were used toassess the accuracy of HM-400 and the mean standarddeviation of the size data was 0.19. The differencesbetween the fragment size means for samples sizedusing HM-400 and commercial size standard were smallwith an average of 0.29 bp. The production cost of HM-400 was only 10% of the cost of commercial size standard.</abstract>

The in-house production of fluorescently labelledinternal size standard offers the advantage of cost savingover the commercial size standard in microsatellitegenotyping. Based on the reported in-house internal sizestandard protocol, we have improved the method by generating21 DNA fragments (in a standard named as HM-400) with each size similar to that of the commercialsize standard. The consistent amplification of the correctfragment size was optimised via primer modulationfor non-templated nucleotide addition by Taq DNA polymerase.A total of six microsatellite loci were used toassess the accuracy of HM-400 and the mean standarddeviation of the size data was 0.19. The differencesbetween the fragment size means for samples sizedusing HM-400 and commercial size standard were smallwith an average of 0.29 bp. The production cost of HM-400 was only 10% of the cost of commercial size standard.

Short Note: Internal Size Standard for Microsatellite Genotyping

Silvae Genetica

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

{"article-title":"Short Note: Internal Size Standard for Microsatellite Genotyping"}

The in-house production of fluorescently labelledinternal size standard offers the advantage of cost savingover the commercial size standard in...

Short Note: Internal Size Standard for Microsatellite Genotyping

Pubblicato online: 19 ott 2017

Pagine: 94 - 96

Ricevuto: 17 mar 2008

DOI: https://doi.org/10.1515/sg-2009-0012

Parole chiave
size standard, microsatellites, genotyping, population genetics, dipterocarps.

© 2017

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Short Note: Internal Size Standard for Microsatellite Genotyping

Pubblicato online: 19 ott 2017

Pagine: 94 - 96

Ricevuto: 17 mar 2008

DOI: https://doi.org/10.1515/sg-2009-0012

Parole chiavesize standard, microsatellites, genotyping, population genetics, dipterocarps.

© 2017

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Parole chiave
size standard, microsatellites, genotyping, population genetics, dipterocarps.