<abstract xmlns="http://www.w3.org/1999/xhtml"><p> Microsatellites are widely used markers for multiple purposes in oaks. We describe a complete procedure for cheap DNA extraction and fast microsatellites genotyping by multiplex PCR. 10 loci were selected to form two multiplex kits including three loci that show a high differentiation between Quercus robur and Q. petraea. The loci were tested in three oak species and show a high mean genetic diversity of 0.84. The cumulative exclusion probability for parentage analysis was 0.999977 for single parent and 1.0 for paternity. Finally, the relatively high differentiation coefficient (Gst = 0.04) will facilitate species assignment based on genotypes in oaks.</p></abstract>

Microsatellites are widely used markers for multiple purposes in oaks. We describe a complete procedure for cheap DNA extraction and fast microsatellites genotyping by multiplex PCR. 10 loci were selected to form two multiplex kits including three loci that show a high differentiation between Quercus robur and Q. petraea. The loci were tested in three oak species and show a high mean genetic diversity of 0.84. The cumulative exclusion probability for parentage analysis was 0.999977 for single parent and 1.0 for paternity. Finally, the relatively high differentiation coefficient (Gst = 0.04) will facilitate species assignment based on genotypes in oaks.

Short Note: High Throughput Microsatellite Genotyping in Oak Species

Silvae Genetica

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

{"article-title":"Short Note: High Throughput Microsatellite Genotyping in Oak Species"}

Microsatellites are widely used markers for multiple purposes in oaks. We describe a complete procedure for cheap DNA extraction and fast microsatellites...

Short Note: High Throughput Microsatellite Genotyping in Oak Species

Pubblicato online: 19 ott 2017

Pagine: 238 - 240

Ricevuto: 21 mar 2006

DOI: https://doi.org/10.1515/sg-2006-0031

Parole chiave
microsatellites, multiplex PCR, Quercus, genetic assignment, parentage analysis

© 2017

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Short Note: High Throughput Microsatellite Genotyping in Oak Species

Pubblicato online: 19 ott 2017

Pagine: 238 - 240

Ricevuto: 21 mar 2006

DOI: https://doi.org/10.1515/sg-2006-0031

Parole chiavemicrosatellites, multiplex PCR, Quercus, genetic assignment, parentage analysis

© 2017

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Parole chiave
microsatellites, multiplex PCR, Quercus, genetic assignment, parentage analysis