<abstract xmlns="http://www.w3.org/1999/xhtml"><p> We describe the development of a SCAR-marker linked to low extractives content of Norway Spruce (Picea abies L [Karst.]) derived from AFLPs. In these analyses 57 different primer enzyme combinations were used in a bulked segregant analysis approach comparing individuals with high and low extractives content. A total of 14 polymorphic AFLP markers were detected between the pools. Five markers were selected for further analyses to verify their linkage to extractives content based on individuals used for pool constitution. One AFLP marker, found to be significant linked to low extractives content was converted into a SCAR marker for further validation. For this marker, a monomorphic band was obtained by using sets of nested primers or restriction site specific primers (RSS) which include the AFLP-restriction recognition site. The separation of the marker from unlinked size homologous marker-alleles was realized by a SSCP-approach. Validation of the marker on different full-sib families confirmed the usability to separate the classes for low and high extractives content of Picea abies.</p></abstract>

We describe the development of a SCAR-marker linked to low extractives content of Norway Spruce (Picea abies L [Karst.]) derived from AFLPs. In these analyses 57 different primer enzyme combinations were used in a bulked segregant analysis approach comparing individuals with high and low extractives content. A total of 14 polymorphic AFLP markers were detected between the pools. Five markers were selected for further analyses to verify their linkage to extractives content based on individuals used for pool constitution. One AFLP marker, found to be significant linked to low extractives content was converted into a SCAR marker for further validation. For this marker, a monomorphic band was obtained by using sets of nested primers or restriction site specific primers (RSS) which include the AFLP-restriction recognition site. The separation of the marker from unlinked size homologous marker-alleles was realized by a SSCP-approach. Validation of the marker on different full-sib families confirmed the usability to separate the classes for low and high extractives content of Picea abies.

Identification of Molecular Markers for Selected Wood Properties of Norway Spruce Picea abies L. (Karst.) II. Extractives Content

Silvae Genetica

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

We describe the development of a SCAR-marker linked to low extractives content of Norway Spruce (Picea abies L [Karst.]) derived from AFLPs. In these analyses...

Identification of Molecular Markers for Selected Wood Properties of Norway Spruce Picea abies L. (Karst.) II. Extractives Content

Pubblicato online: 19 ott 2017

Pagine: 145 - 152

Ricevuto: 26 gen 2003

DOI: https://doi.org/10.1515/sg-2005-0022

Parole chiave
Bulked segregant analysis, AFLP, Molecular markers, Marker assisted selection (MAS), Wood properties, Marker conversion, SCAR marker

© 2017

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Identification of Molecular Markers for Selected Wood Properties of Norway Spruce Picea abies L. (Karst.) II. Extractives Content

Pubblicato online: 19 ott 2017

Pagine: 145 - 152

Ricevuto: 26 gen 2003

DOI: https://doi.org/10.1515/sg-2005-0022

Parole chiaveBulked segregant analysis, AFLP, Molecular markers, Marker assisted selection (MAS), Wood properties, Marker conversion, SCAR marker

© 2017

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Parole chiave
Bulked segregant analysis, AFLP, Molecular markers, Marker assisted selection (MAS), Wood properties, Marker conversion, SCAR marker