<abstract xmlns="http://www.w3.org/1999/xhtml"> The morphology of the eggs and miracidia of Eurytrema coelomaticum was analyzed. The eggshell presented different composition according to the maturation stage of the egg, as seen by distinct birefringence after polarized light microscopic analysis. Detailed morphology of the brownish eggs, measuring 44.97±3.83 (38.67 - 51.40) μm in length and 30.71±2.54 (25.79 -34.47) μm in width, and the different electrondensities of the inner, middle and outer layers of the eggs were determined using light (bright field and DIC) and electronic microscopy (scanning and transmission). The morphometry of the eggs is in accordance to those measures presented in previous studies. The miracidia were observed using light microscopy, inside and outside the egg. It was possible to observe the terebratorium at the anterior end of the body, the ciliated epidermal plates, placed according the formula E=2E1 + 2E2, and the interepidermal ridge, but eyespots were not observed. The excretory vesicle was laterally placed in the posterior middle of the body and the germ balls were observed. The miracidium inside the egg was observed by routine techniques, but the miracidium was not well preserved. Thus, the eggs were processed using high pressure-freeze substitution technique and reasonable preservation was achieved. The cilia covering the larval body, with a typical ciliary organization, the terebratorium with folds and the junction of the epidermal ciliated cell with the larval body were seen. For the first time detailed information on the eggs and miracidia of E. coelomaticum is given, which can be the basis for new studies.</abstract>

The morphology of the eggs and miracidia of Eurytrema coelomaticum was analyzed. The eggshell presented different composition according to the maturation stage of the egg, as seen by distinct birefringence after polarized light microscopic analysis. Detailed morphology of the brownish eggs, measuring 44.97±3.83 (38.67 - 51.40) μm in length and 30.71±2.54 (25.79 -34.47) μm in width, and the different electrondensities of the inner, middle and outer layers of the eggs were determined using light (bright field and DIC) and electronic microscopy (scanning and transmission). The morphometry of the eggs is in accordance to those measures presented in previous studies. The miracidia were observed using light microscopy, inside and outside the egg. It was possible to observe the terebratorium at the anterior end of the body, the ciliated epidermal plates, placed according the formula E=2E1 + 2E2, and the interepidermal ridge, but eyespots were not observed. The excretory vesicle was laterally placed in the posterior middle of the body and the germ balls were observed. The miracidium inside the egg was observed by routine techniques, but the miracidium was not well preserved. Thus, the eggs were processed using high pressure-freeze substitution technique and reasonable preservation was achieved. The cilia covering the larval body, with a typical ciliary organization, the terebratorium with folds and the junction of the epidermal ciliated cell with the larval body were seen. For the first time detailed information on the eggs and miracidia of E. coelomaticum is given, which can be the basis for new studies.

Additional study of the morphology of eggs and miracidia of Eurytrema coelomaticum (Trematoda)

Área de Biofísica, Departamento de Ciências Fisiológicas, Instituto de Biologia, UFRRJ, Seropédica, RJ, Brazil

Curso de Pós-Graduação em Ciências Veterinárias, Departamento de Parasitologia Animal, Instituto de Veterinária, UFRRJ, Seropédica, RJ. Secretaria Municipal de Educação do Rio de Janeiro, Prefeitura da Cidade do Rio de Janeiro, Rio de Janeiro, RJ, Brazil

Pólo Cidade Universitária, Campus Universidade Federal do Rio de Janeiro, Macaé, Av. Aluizio da Silva Gomes, 50, Granja dos Cavaleiros, Macaé, RJ, Brazil

Área de Biologia, Departamento de Biologia Animal, Instituto de Biologia, UFRRJ, Seropédica, RJ, Brazil

Curso de Pós-Graduação em Biofísica, Laboratório de Biologia de Helmintos Otto Wucherer, Programa de Biologia Celular e Parasitologia, Instituto de Biofísica Carlos Chagas Filho, Centro de Ciências da Saúde, UFRJ, Rio de Janeiro, RJ, Brazil

Laboratório de Helmintologia Romero Lascasas Porto, Departamento de Microbiologia, Imunologia e Parasitologia da Faculdade de Ciências Médicas, UERJ, RJ, Brazil

Laboratório de Biologia Celular e Tecidual, Centro de Biologia e Biotecnologia, UENF, Campos dos Goytacazes, RJ, Brazil

Laboratório de Ultraestrura Celular Hertha Meyer, Programa de Biologia Celular e Parasitologia, Instituto de Biofísica Carlos Chagas Filho, Centro de Ciências da Saúde, UFRJ, Rio de Janeiro, RJ, Brazil

Helminthologia

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The morphology of the eggs and miracidia of Eurytrema coelomaticum was analyzed. The eggshell presented different composition according to the maturation stage...

Additional study of the morphology of eggs and miracidia of Eurytrema coelomaticum (Trematoda)

Pubblicato online: 13 ago 2015

Pagine: 244 - 251

Ricevuto: 15 gen 2015

Accettato: 12 mar 2015

DOI: https://doi.org/10.1515/helmin-2015-0039

© Institute of Parasitology, SAS, Košice

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.