<abstract xmlns="http://www.w3.org/1999/xhtml">The microbial degradation of vinyl acetate (VA) by <italic>Pseudomonas fluorescens</italic> PCM 2123 strain was studied in both batch and continuous modes. The purpose of the experiments was to determine the kinetic model of the cell growth and biodegradation rate of vinyl acetate (VA), which was the sole carbon and energy source for tested microorganisms. The experiments, carried out in a batch reactor for several initial concentrations of growth substrate in the liquid phase ranging from 18.6 to 373 gsubstrate·m−3 (gs·m−3) made it possible to choose the kinetic model and to estimate its constants. The Haldane inhibitory model with the values of constants: <italic>μm</italic> = 0.1202 h−1, <italic>KS</italic> = 17.195 gs·m−3, <italic>Ki</italic> = 166.88 gs·m−3 predicted the experimental data with the best accuracy. To set the parameters of maintenance metabolism it was necessary to carry out a series of continuous cultures at different dilution rates (0.05 to 0.072 h−1) and concentrations of VA in the liquid supplied to the chemostat ranging from 30.9 to 123.6 gs·m−3. The obtained data-base enabled to determine the coefficient for maintenance metabolism (<italic>me</italic> = 0.0251 gsubstrate gcell dry weight−1·h−1 (gs·gcdw−1·h−1)) as well as the maximal and observed values of yield coefficients, <italic>Yxs M</italic> = 0.463 gcdw·gs−1 and (<italic>Yxs</italic>)<italic>obs</italic> = 0.411 gcdw·gs−1, respectively. The developed kinetics was verified by comparison of the computed and obtained in batch experiments profiles of changes in biomass and growth substrate concentrations.</abstract>

The microbial degradation of vinyl acetate (VA) by Pseudomonas fluorescens PCM 2123 strain was studied in both batch and continuous modes. The purpose of the experiments was to determine the kinetic model of the cell growth and biodegradation rate of vinyl acetate (VA), which was the sole carbon and energy source for tested microorganisms. The experiments, carried out in a batch reactor for several initial concentrations of growth substrate in the liquid phase ranging from 18.6 to 373 gsubstrate·m−3 (gs·m−3) made it possible to choose the kinetic model and to estimate its constants. The Haldane inhibitory model with the values of constants: μm = 0.1202 h−1, KS = 17.195 gs·m−3, Ki = 166.88 gs·m−3 predicted the experimental data with the best accuracy. To set the parameters of maintenance metabolism it was necessary to carry out a series of continuous cultures at different dilution rates (0.05 to 0.072 h−1) and concentrations of VA in the liquid supplied to the chemostat ranging from 30.9 to 123.6 gs·m−3. The obtained data-base enabled to determine the coefficient for maintenance metabolism (me = 0.0251 gsubstrate gcell dry weight−1·h−1 (gs·gcdw−1·h−1)) as well as the maximal and observed values of yield coefficients, Yxs M = 0.463 gcdw·gs−1 and (Yxs)obs = 0.411 gcdw·gs−1, respectively. The developed kinetics was verified by comparison of the computed and obtained in batch experiments profiles of changes in biomass and growth substrate concentrations.

Kinetics of Vinyl Acetate Biodegradation by <italic>Pseudomonas fluorescens</italic> PCM 2123

Institute of Chemical Engineering, Polish Academy of Sciences

Department of Biochemistry, Faculty of Biology and Environment Protection

Ecological Chemistry and Engineering S

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Kinetics of Vinyl Acetate Biodegradation by  PCM 2123

The microbial degradation of vinyl acetate (VA) by Pseudomonas fluorescens PCM 2123 strain was studied in both batch and continuous modes. The purpose of the...

Kinetics of Vinyl Acetate Biodegradation by Pseudomonas fluorescens PCM 2123

Pubblicato online: 23 ott 2018

Pagine: 487 - 502

DOI: https://doi.org/10.1515/eces-2018-0033

Parole chiave
vinyl acetate, kinetics, batch and continuous cultures

© 2018 Agnieszka Gąszczak et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Kinetics of Vinyl Acetate Biodegradation by Pseudomonas fluorescens PCM 2123

Pubblicato online: 23 ott 2018

Pagine: 487 - 502

DOI: https://doi.org/10.1515/eces-2018-0033

Parole chiavevinyl acetate, kinetics, batch and continuous cultures

© 2018 Agnieszka Gąszczak et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Parole chiave
vinyl acetate, kinetics, batch and continuous cultures