Volume 70 (2019): Issue 3 (December 2019) Special Issue: with invited papers from the 47th International Symposium „Actual tasks on Agricultural Engineering“ (ATAE), 5th – 7th March 2019, Opatija, Croatia, http://atae.agr.hr/, Editor: Prof. Andreas Gronauer
The aim of this study was to investigate the effects of garlic oil, cinnamaldehyde, carvacrol, thymol, and thyme oil on growth and biofilm formation of Escherichia coli and Salmonella serotypes, including field isolates from livestock animals. Minimum inhibitory concentrations (MIC) were determined using broth micro-dilution method. Biofilm biomass was assessed by measuring the attached biomass with microtiter plate assay and crystal violet (CV) staining. The strongest antimicrobial effects on E. coli serotypes were observed for thymol at 150 ppm, followed by carvacrol and cinnamaldehyde at 300 ppm and thyme oil at 600 ppm. Similar results were obtained with Salmonella serotypes except for carvacrol (MIC value at 150 ppm). Garlic oil showed no growth inhibition on serotypes of E. coli and Salmonella up to 10000 ppm. Cinnamaldehyde proved to be the most effective substance in reducing E. coli CV-biofilm formation at sub-MIC level with a threshold concentration of 5 ppm, followed by carvacrol, thymol, and thyme oil at 40 ppm and garlic oil at 10000 ppm. CV-biofilm formation of Salmonella serotypes at sub-MIC level was clearly reduced with 40 ppm cinnamaldehyde and 80 ppm carvacrol, thymol, and thyme oil. No reduction of CV-biofilm formation was observed with garlic oil. The present study demonstrates a strong antibacterial activity of cinnamaldehyde, carvacrol, thymol, and thyme oil. Similar response of field isolates and type strains to these phytogenics suggests a general effect within the bacterial species tested. All four substances were also able to reduce CV-biofilm formation at sub-MIC level. Investigating phytogenics with bacterial field isolates contributes to the development of feed additives as alternatives to antibiotics in animal feed to increase productivity and animal welfare in modern livestock production.
A 72-day feeding trial was conducted at Sagana fish farm to assess the effects of substitution of fish meal (FM) with black soldier fly larvae meal (BSFLM) on the growth performance and survival rates of Nile tilapia. Four test diets were formulated and substitution of FM by BSFLM made at 0%, 33%, 67% and 100%. 240 male Nile tilapia (52.3 ± 0.29 g) were divided into 4 groups (4 replicates) each and were placed in 16 hapa nets (15 fish/hapa), mounted in an 800 m2 earthen pond. Fish were fed twice per day at 5% of the body weight. Sampling of the fish was done biweekly; mortalities were recorded daily while the physico-chemical parameters were monitored weekly. The growth performance and survival rates of the Nile tilapia were not significantly different (p > 0.05) between treatments. In conclusion, the present study indicates that full fat BSFLM can replace up to 100% of the FM without negative effects on the growth performance and survival rates of Nile tilapia, although the slight trend towards reduced growth for the 100% substitution diet points at the need for future studies on increasing the nutritional value of BSFLM, for example, by further processing.
In keeping of breeding sows, it is important to use the working time as efficiently as possible to ensure economics within legal production conditions. This can be supported by providing working time requirement data. Therefore, the aim of this study was to generate working time requirement of current group keeping systems in the waiting area of breeding sows. For this purpose, in the keeping systems for small groups with feeding bay and large groups with retrieval station, data was collected using mobile video technology and semi-standardized questionnaires at four farms with current technology. With the gained data, operating models were created under optimized conditions in order to display the working time requirement of work processes for different stock sizes. Both keeping systems differed significantly from each other in the total working time requirement over different stock sizes. The total working time requirement in the waiting area with a typical herd size of 140 breeding sows of Austrian growing farms was 3.58 MPh/sow/year for the small group and 2.68 MPh/sow/year for the large group. The comparison of the available results with data from previous studies indicated the trend of decrease in working hours per sow and year. This was due to technical progress which enabled larger herd sizes.
In addition to soil structure and pore function disturbances, the impairments of natural soil functions on line construction sites (e. g. power cable routing) also include changes in the site-specific variable thermal-hydraulic properties in the area of the line trenches. Based on the pore size distribution values as a function of dry bulk density classes compiled in German Soil Classification system “KA5” (Ad-Hoc-AG-Boden, 2005), volumetric heat capacity (C), thermal conductivity (λ), and thermal diffusivity (k) can be calculated using the model of Xie et al. (2018). These calculated data are presented for the soil texture spectrum according to KA5, as well for selected textures (Sl3, Lt2, Tu3) and class ranges of dry bulk density (ρt).
The typical curves for C (linear increase), λ (increasingly flattening increase) and k (increase with reversal to negative slope) and their rise with increasing ρt (1.1–1.7 g cm−3) are shown. k varies depending on the proportions of clay, silt and sand within the soil texture class. The curve shapes between low and high ρt differ more for “Sl3” than for the finer-grained texture class “Tu3”. With the help of these data sets, changes in the heat balance and the thermal- hydraulic soil properties can be predicted for line projects in the power grid expansion before the start of construction measures, if soil texture and dry bulk density are determined by field description methods.
In the H2020 project FATIMA (FArming Tools for external nutrient Inputs and water MAnagement) an integrated approach for the optimisation of nitrogen management in agriculture was applied. In addition to the technical component, which included the processing of Sentinel-2 satellite data, the socio-economic and agronomic aspects of improved nutrient management were investigated. The results show that farmers are mainly interested in timely application maps for variable-rate nitrogen application. In the course of the project not only a field trial for the validation of satellite data as a basis for an economic analysis was established, but also an online application for site-specific fertilisation based on current and historical satellite images was developed and tested.
The aim of this study was to investigate the effects of garlic oil, cinnamaldehyde, carvacrol, thymol, and thyme oil on growth and biofilm formation of Escherichia coli and Salmonella serotypes, including field isolates from livestock animals. Minimum inhibitory concentrations (MIC) were determined using broth micro-dilution method. Biofilm biomass was assessed by measuring the attached biomass with microtiter plate assay and crystal violet (CV) staining. The strongest antimicrobial effects on E. coli serotypes were observed for thymol at 150 ppm, followed by carvacrol and cinnamaldehyde at 300 ppm and thyme oil at 600 ppm. Similar results were obtained with Salmonella serotypes except for carvacrol (MIC value at 150 ppm). Garlic oil showed no growth inhibition on serotypes of E. coli and Salmonella up to 10000 ppm. Cinnamaldehyde proved to be the most effective substance in reducing E. coli CV-biofilm formation at sub-MIC level with a threshold concentration of 5 ppm, followed by carvacrol, thymol, and thyme oil at 40 ppm and garlic oil at 10000 ppm. CV-biofilm formation of Salmonella serotypes at sub-MIC level was clearly reduced with 40 ppm cinnamaldehyde and 80 ppm carvacrol, thymol, and thyme oil. No reduction of CV-biofilm formation was observed with garlic oil. The present study demonstrates a strong antibacterial activity of cinnamaldehyde, carvacrol, thymol, and thyme oil. Similar response of field isolates and type strains to these phytogenics suggests a general effect within the bacterial species tested. All four substances were also able to reduce CV-biofilm formation at sub-MIC level. Investigating phytogenics with bacterial field isolates contributes to the development of feed additives as alternatives to antibiotics in animal feed to increase productivity and animal welfare in modern livestock production.
A 72-day feeding trial was conducted at Sagana fish farm to assess the effects of substitution of fish meal (FM) with black soldier fly larvae meal (BSFLM) on the growth performance and survival rates of Nile tilapia. Four test diets were formulated and substitution of FM by BSFLM made at 0%, 33%, 67% and 100%. 240 male Nile tilapia (52.3 ± 0.29 g) were divided into 4 groups (4 replicates) each and were placed in 16 hapa nets (15 fish/hapa), mounted in an 800 m2 earthen pond. Fish were fed twice per day at 5% of the body weight. Sampling of the fish was done biweekly; mortalities were recorded daily while the physico-chemical parameters were monitored weekly. The growth performance and survival rates of the Nile tilapia were not significantly different (p > 0.05) between treatments. In conclusion, the present study indicates that full fat BSFLM can replace up to 100% of the FM without negative effects on the growth performance and survival rates of Nile tilapia, although the slight trend towards reduced growth for the 100% substitution diet points at the need for future studies on increasing the nutritional value of BSFLM, for example, by further processing.
In keeping of breeding sows, it is important to use the working time as efficiently as possible to ensure economics within legal production conditions. This can be supported by providing working time requirement data. Therefore, the aim of this study was to generate working time requirement of current group keeping systems in the waiting area of breeding sows. For this purpose, in the keeping systems for small groups with feeding bay and large groups with retrieval station, data was collected using mobile video technology and semi-standardized questionnaires at four farms with current technology. With the gained data, operating models were created under optimized conditions in order to display the working time requirement of work processes for different stock sizes. Both keeping systems differed significantly from each other in the total working time requirement over different stock sizes. The total working time requirement in the waiting area with a typical herd size of 140 breeding sows of Austrian growing farms was 3.58 MPh/sow/year for the small group and 2.68 MPh/sow/year for the large group. The comparison of the available results with data from previous studies indicated the trend of decrease in working hours per sow and year. This was due to technical progress which enabled larger herd sizes.
In addition to soil structure and pore function disturbances, the impairments of natural soil functions on line construction sites (e. g. power cable routing) also include changes in the site-specific variable thermal-hydraulic properties in the area of the line trenches. Based on the pore size distribution values as a function of dry bulk density classes compiled in German Soil Classification system “KA5” (Ad-Hoc-AG-Boden, 2005), volumetric heat capacity (C), thermal conductivity (λ), and thermal diffusivity (k) can be calculated using the model of Xie et al. (2018). These calculated data are presented for the soil texture spectrum according to KA5, as well for selected textures (Sl3, Lt2, Tu3) and class ranges of dry bulk density (ρt).
The typical curves for C (linear increase), λ (increasingly flattening increase) and k (increase with reversal to negative slope) and their rise with increasing ρt (1.1–1.7 g cm−3) are shown. k varies depending on the proportions of clay, silt and sand within the soil texture class. The curve shapes between low and high ρt differ more for “Sl3” than for the finer-grained texture class “Tu3”. With the help of these data sets, changes in the heat balance and the thermal- hydraulic soil properties can be predicted for line projects in the power grid expansion before the start of construction measures, if soil texture and dry bulk density are determined by field description methods.
In the H2020 project FATIMA (FArming Tools for external nutrient Inputs and water MAnagement) an integrated approach for the optimisation of nitrogen management in agriculture was applied. In addition to the technical component, which included the processing of Sentinel-2 satellite data, the socio-economic and agronomic aspects of improved nutrient management were investigated. The results show that farmers are mainly interested in timely application maps for variable-rate nitrogen application. In the course of the project not only a field trial for the validation of satellite data as a basis for an economic analysis was established, but also an online application for site-specific fertilisation based on current and historical satellite images was developed and tested.