<abstract xmlns="http://www.w3.org/1999/xhtml">

<p>β-D-glucosidase (βG) gene from <italic>Oenococcus oeni</italic> SD-2a and 31MBR was cloned, sequenced and analyzed, also intracellular βG of the two strains was further localized. The results showed that βG gene of the two strains was in high homology (> 99%) to reported βG gene, confirming both strains possess βG activity at the molecular level. Intracellular βG of SD-2a is a mainly soluble protein, existing mostly in the cytoplasm and to some extent in the periplasm. While for 31MBR, intracellular βG is mainly insoluble protein existing in the cytoplasmic membrane. This study provides basic information for further study of the metabolic mechanism of βG from <italic>O. oeni</italic> SD-2a and 31MBR.</p>
</abstract>

β-D-glucosidase (βG) gene from Oenococcus oeni SD-2a and 31MBR was cloned, sequenced and analyzed, also intracellular βG of the two strains was further localized. The results showed that βG gene of the two strains was in high homology (> 99%) to reported βG gene, confirming both strains possess βG activity at the molecular level. Intracellular βG of SD-2a is a mainly soluble protein, existing mostly in the cytoplasm and to some extent in the periplasm. While for 31MBR, intracellular βG is mainly insoluble protein existing in the cytoplasmic membrane. This study provides basic information for further study of the metabolic mechanism of βG from O. oeni SD-2a and 31MBR.

Identification and Localization of <italic>β</italic>-D-Glucosidase from Two Typical <italic>Oenococcus oeni</italic> Strains

Polish Journal of Microbiology

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Identification and Localization of -D-Glucosidase from Two Typical  Strains

β-D-glucosidase (βG) gene from Oenococcus oeni SD-2a and 31MBR was cloned, sequenced and analyzed, also intracellular βG of the two strains was further...

Identification and Localization of β-D-Glucosidase from Two Typical Oenococcus oeni Strains

Publié en ligne: 07 juin 2016

Pages: 209 - 213

Reçu: 02 mars 2015

Accepté: 06 sept. 2015

DOI: https://doi.org/10.5604/17331331.1204481

Mots clés
β-D-glucosidase, βG cloned, gene localization

© 2016 YAHUI LI et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Identification and Localization of β-D-Glucosidase from Two Typical Oenococcus oeni Strains

Publié en ligne: 07 juin 2016

Pages: 209 - 213

Reçu: 02 mars 2015

Accepté: 06 sept. 2015

DOI: https://doi.org/10.5604/17331331.1204481

Mots clésβ-D-glucosidase, βG cloned, gene localization

© 2016 YAHUI LI et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Mots clés
β-D-glucosidase, βG cloned, gene localization