<abstract xmlns="http://www.w3.org/1999/xhtml">

<p>Several studies have observed that use of a conventional PCR protocol with primers LM1 and LM2 for the identification of the <italic>hly</italic>A gene of <italic>Listeria monocytogenes</italic> generates non-specific PCR amplifications and false positives. For this reason, in this study we provide a modified PCR protocol that improves the specificity of the results obtained with LM1 and LM2 primers.</p>
</abstract>

Several studies have observed that use of a conventional PCR protocol with primers LM1 and LM2 for the identification of the hlyA gene of Listeria monocytogenes generates non-specific PCR amplifications and false positives. For this reason, in this study we provide a modified PCR protocol that improves the specificity of the results obtained with LM1 and LM2 primers.

An Improve Protocol for PCR Using LM1 and LM2 Primers for <italic>Listeria monocytogenes</italic> Detection in Food Matrices

Polish Journal of Microbiology

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

An Improve Protocol for PCR Using LM1 and LM2 Primers for  Detection in Food Matrices

Several studies have observed that use of a conventional PCR protocol with primers LM1 and LM2 for the identification of the hlyA gene of Listeria...

An Improve Protocol for PCR Using LM1 and LM2 Primers for Listeria monocytogenes Detection in Food Matrices

Publié en ligne: 28 juin 2017

Pages: 255 - 257

Reçu: 05 août 2016

Accepté: 12 janv. 2017

DOI: https://doi.org/10.5604/01.3001.0010.7869

© 2017 ANGÉLICA GODÍNEZ-OVIEDO et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.