<abstract xmlns="http://www.w3.org/1999/xhtml">

<p>Several studies have observed that use of a conventional PCR protocol with primers LM1 and LM2 for the identification of the <italic>hly</italic>A gene of <italic>Listeria monocytogenes</italic> generates non-specific PCR amplifications and false positives. For this reason, in this study we provide a modified PCR protocol that improves the specificity of the results obtained with LM1 and LM2 primers.</p>
</abstract>

Several studies have observed that use of a conventional PCR protocol with primers LM1 and LM2 for the identification of the hlyA gene of Listeria monocytogenes generates non-specific PCR amplifications and false positives. For this reason, in this study we provide a modified PCR protocol that improves the specificity of the results obtained with LM1 and LM2 primers.

An Improve Protocol for PCR Using LM1 and LM2 Primers for <italic>Listeria monocytogenes</italic> Detection in Food Matrices

Polish Journal of Microbiology

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

An Improve Protocol for PCR Using LM1 and LM2 Primers for  Detection in Food Matrices

Several studies have observed that use of a conventional PCR protocol with primers LM1 and LM2 for the identification of the hlyA gene of Listeria...

An Improve Protocol for PCR Using LM1 and LM2 Primers for Listeria monocytogenes Detection in Food Matrices

Published Online: Jun 28, 2017

Page range: 255 - 257

Received: Aug 05, 2016

Accepted: Jan 12, 2017

DOI: https://doi.org/10.5604/01.3001.0010.7869

© 2017 ANGÉLICA GODÍNEZ-OVIEDO et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.