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Association between single nucleotide polymorphisms and viral load in congenital cytomegalovirus infection

Dominika Jedlińska-Pijanowska
Dominika Jedlińska-Pijanowska
, 
Beata Kasztelewicz
Beata Kasztelewicz
, 
Anna Dobrzańska
Anna Dobrzańska
, 
Katarzyna Dzierżanowska-Fangrat
Katarzyna Dzierżanowska-Fangrat
, 
Maciej Jaworski
Maciej Jaworski
 et 
Justyna Czech-Kowalska
Justyna Czech-Kowalska
   | 16 juil. 2021
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Article Category: Original article
Publié en ligne: 16 juil. 2021
Pages: 9 - 17
DOI: https://doi.org/10.34763/jmotherandchild.20202404.d-20-00014
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© 2021 Dominika Jedlińska-Pijanowska et al. published by Sciendo
This work is licensed under the Creative Commons Attribution 4.0 International License.

Figure 1

HCMV VL in urine depending on genotypes of IL12B rs3212227 polymorphism. Bars in the box show the first and the third quartiles. Whisker charts represent the minimum to maximum values. Small box represents median value. p-value below 0.05 is statistically significant. Kruskal-Wallis ANOVA test was used. HCMV: human cytomegalovirus; VL: viral load.
HCMV VL in urine depending on genotypes of IL12B rs3212227 polymorphism. Bars in the box show the first and the third quartiles. Whisker charts represent the minimum to maximum values. Small box represents median value. p-value below 0.05 is statistically significant. Kruskal-Wallis ANOVA test was used. HCMV: human cytomegalovirus; VL: viral load.

Maternal and neonatal demographic data and general characteristics of cCMV-infected infants, with subgroups of symptomatic and asymptomatic infants.

Characteristics cCMV-infected infants (n = 92) Symptomatic infants (n = 73) Asymptomatic infants (n = 19) p-value

p-value for comparison between symptomatic and asymptomatic infants.
Maternal age at delivery, (years) 28 (25–31) 29 (25–31) 28 (25–31) NS
Primipara, n (%) 42 (45.65) 33 (45.21) 9 (47.37) NS
Preterm delivery (<37Hbd), n (%) 23 (25.00) 19 (26.03) 4 (21.05) NS
Caesarean section, n (%) 49 (54.44) 45 (61.64) 4 (21.05) 0.006
Prenatal HCMV screening, n (%) 37 (40.22) 27 (36.99) 10 (52.63) NS
Birth weight, (g) (21702755 –3200) (21502685 –3140) (26803050 –3630) 0.028
Gestational age, (weeks) 38 (37–39) 38 (37–39) 39 (37–40) NS
Male, n (%) 46 (50.00) 36 (49.32) 10 (52.63) NS
Age at admission to NICU, Median (IQR), (days) 16 (9–23) 14 (9–21) 22 (20–35) <0.001
Weight at admission to NICU, (g) 3040 (2440–3450) 2980 (2340–3320) 3600 (2930–4310) <0.001
Age at first HCMV DNA detection in urine, (days) 9 (4–15) 8 (3–14) 17 (8–20) 0.002

Allele frequencies of examined SNPs in the study population of cCMV-infected neonates and subgroups of symptomatic and asymptomatic neonates

SNP,rs number

SNP database (dbSNP) reference number (ID number).

, alleles		 Allele cCMV-infected infants (n = 92) Symptomatic infants (n = 73) Asymptomatic infants (n = 19) p-value

p-value for comparison between frequencies of alleles and subgroups of symptomatic and asymptomatic CMV-infected newborn infants.
Allele frequencies, n (%)
IL1B G 121 (65.76) 97 (66.44) 24 (63.16)
rs16944 NS
G/A A 63 (34.24) 49 (33.56) 14 (36.84)
IL12B T 142 (77.17) 110 (75.34) 32 (84.21)
rs3212227 NS
G/T G 42 (22.83) 36 (24.66) 6 (15.79)
IL28B C 120 (65.22) 92 (63.01) 28 (73.68)
rs12979860 NS
C/T T 64 (34.78) 54 (36.99) 10 (26.32)
CCL2 A 139 (75.54) 110 (75.34) 29 (76.32)
rs1024611 NS
A/G G 45 (24.46) 36 (24.66) 9 (23.68)
DC-SIGN G 107 (58.15) 87 (59.59) 20 (52.63)
rs735240 NS
A/G A 77 (41.85) 59 (40.41) 18 (47.37)
TLR2 G 174 (94.57) 138 (94.52) 36 (94.74)
rs5743708 NS
A/G A 10 (5.43) 8 (5.48) 2 (5.26)
TLR4 C 175 (95.11) 139 (95.21) 36 (94.74)
rs4986791 NS
C/T T 9 (4.89) 7 (4.79) 2 (5.26)
TLR9 T 107 (58.15) 84 (57.53) 23 (60.53)
rs352140 NS
C/T C 77 (41.85) 62 (42.47) 15 (39.47)

Allele frequencies of examined SNPs in the study population in comparison to known European and Global reference populations from the Reference SNP (rs) Report

SNP,rs number

SNP database (dbSNP) reference number (ID number).

, alleles		 Allele All study population (n = 233) Uninfected control infants (n = 141) cCMV-infected infants (n = 92) p-value

p-value for comparison between frequencies of alleles and subgroups of uninfected controls and CMV-infected newborn infants.

 Reference population

Reference allele frequency based on 1000Genomes study from the Reference SNP (rs) Report.
European
 Global
Allele frequencies, n (%) Allele frequencies, %
IL1B G 320 (68.67) 199 (70.57) 121 (65.76) 65.01 49.06
rs16944 NS
G/A A 146 (31.33) 83 (29.43) 63 (34.24) 34.99 50.94
IL12B T 352(75.54) 210 (74.47) 142 (77.17) 77.73 64.10
rs3212227 NS
G/T G 114 (24.46) 72 (25.53) 42 (22.83) 22.27 35.90
IL28B C 309 (66.31) 189 (67.02) 120 (65.22) 69.09 64.42
rs12979860 NS
C/T T 157 (33.69) 93 (32.98) 64 (34.78) 30.91 35.58
CCL2 A 344 (73.82) 205 (72.70) 139 (75.54) 68.39 63.64
rs1024611 NS
A/G G 122 (26.18) 77(27.30) 45 (24.46) 31.61 36.36
DC-SIGN G 278 (59.66) 171 (60.64) 107 (58.15) 56.76 64.70
rs735240 NS
A/G A 188 (40.34) 111 (39.36) 77 (41.85) 43.24 35.30
TLR2 G 441 (94.64) 267 (94.68) 174 (94.57) 97.61 99.32
rs5743708 NS
A/G A 25 (5.36) 15 (5.32) 10 (5.43) 2.39 0.68
TLR4 C 440 (94.42) 265 (93.97) 175 (95.11) 94.23 95.93
rs4986791 NS
C/T T 26 (5.58) 17 (6.03) 9 (4.89) 5.77 4.07
TLR9 T 269 (57.73) 162 (57.45) 107 (58.15) 54.57 41.55
rs352140 NS
C/T C 197 (42.27) 120 (42.55) 77 (41.85) 45.43 58.45

Association between alleles of examined polymorphisms (SNPs) and viremia and viruria in asymptomatic newborn infants with cCMV infection

Asymptomatic newborn infants with cCMV infection (N = 19)
SNP, rs number

SNP database (dbSNP) reference number (ID number).

, alleles		 Allele n, (%) HCMV DNA (copies/mL) in blood p-value

p-value – for comparison between alleles and viremia.

 HCMV DNA (copies/mL) in urine p-value

p-value – for comparison between alleles and viruria.
IL1B G 24 (63.16) 2.0 × 103 (7.5 × 102–3.3 × 103) (n = 20) 4.9 ×105 (1.4 × 105–5.3 × 106)
rs16944 NS NS
G/A A 14 (36.84) 1.8 × 103 (9.9 × 102–2.7 × 104) 2.4 ×106 (1.7 × 105–6.1 × 106)
IL12B T 32 (84.21) 1.8 × 103 (9.9 × 102–1.5 × 104) (n = 28) 6.9 ×105 (1.4 × 105–5.3 × 106)
rs3212227 NS NS
G/T G 6 (15.79) 2.1 × 103 (0.0–4.0 × 103) 1.0 ×106 (1.7 × 105–2.0 × 107)
IL28B C 28 (73.68) 2.1 × 103 (6.5 × 102–2.7 × 104) (n = 24) 1.3 ×106 (9.1 × 104–6.1 × 106)
rs12979860 NS NS
C/T T 10 (26.32) 1.2 × 103 (9.9 × 102–2.7 × 103) 2.9 ×105 (2.5 × 105–2.8 × 106)
CCL2 A 29 (76.32) 1.8 × 103 (9.9 × 102–4.0 × 103) (n = 26) 6.9 ×105 (1.7 × 105–6.1 × 106)
rs1024611 NS NS
A/G G 9 (23.68) 2.4 × 103 (9.5 × 102–1.5 × 104) (n = 8) 2.5 ×105 (1.4 × 105–3.2 × 106)
DC-SIGN G 20 (52.63) 1.2 × 103 (1.5 × 102–3.0 × 103) (n = 16) 4.9 ×105 (1.4 × 105–7.5 × 106)
rs735240 NS NS
A/G A 18 (47.37) 2.1 × 103 (1.2 × 103–3.2 × 104) 1.3 ×106 (1.7 × 105–4.6 × 106)
TLR2 G 36 (94.74) 2.0 × 103 (6.5 × 102–1.5 × 104) (n = 32) 1.3 ×106 (1.4 × 105–6.1 × 106)
rs5743708 NS NS
A/G A 2 (5.26) 1.4 × 103 (1.2 × 103–1.6 × 103) 2.3 ×105 (1.7 × 105–2.9 × 105)
TLR4 C 36 (94.74) 2.0 × 103 (9.9 × 102–1.5 × 104) (n = 32) 6.9 ×105 (1.5 × 105–5.3 × 106)
rs4986791 NS NS
C/T T 2 (5.26) 2.0 × 103 (0–4.0 × 103) 1.0 ×107 (0–2.0 × 107)
TLR9 T 23 (60.53) 1.8 × 103 (1.0 × 103–4.0 × 103) (n = 20) 2.9 ×105 (1.4 × 105–6.1 × 106)
rs352140 NS NS
C/T C 15 (39.47) 2.0 × 103 (3.1 × 102–2.7 × 104) (n = 14) 2.8 ×106 (4.0 × 104–6.1 × 106)

Association between genotypes of examined polymorphisms (SNPs) and viremia and viruria in newborn infants with cCMV infection.

SNP, rs number

SNP database (dbSNP) reference number (ID number).

, alleles		 Genotype n = 92, n (%) HCMV DNA (copies/mL) in blood p-value

p-value – for comparison between genotypes and viremia.

 HCMV DNA (copies/mL) in urine p-value

p-value – for comparison between genotypes and viruria.
IL1B A/A 7 (7.6) 6.3 × 103 (3.8 × 103–2.7 × 104) 6.5 × 106 (2.8 × 106–1.0 × 107)
rs16944 A/G 49 (53.3) 8.6 × 103 (1.9 × 103–5.8 × 104) (n = 48) NS 7.8 × 106 (5.1 × 105–1.0 × 107) (n = 48) NS
G/A G/G 36 (39.1) 1.5 × 104 (2.0 × 103–2.0 × 105) (n = 34) 1.0 × 107 (1.7 × 106–1.0 × 107) (n = 35)
IL12B T/T 57 (62.0) 2.2 × 104 (2.1 × 103–1.5 × 105) (n = 54) 5.8 × 106 (6.0 × 105–1.0 × 107) (n = 56)
rs3212227 T/G 28 (30.4) 7.4 × 103 (1.8 × 103–4.7 × 104) NS 1.0 × 107 (6.4 × 106–1.0 × 107) (n = 27) 0.029
G/T G/G 7 (7.6) 4.3 × 103 (2.1 × 103–3.0 × 104) 1.7 × 106 (4.2 × 105–1.0 × 107)
IL28B T/T 13 (14.1) 5.8 × 103 (1.9 × 103–1.3 × 105) (n = 12) 2.8 × 106 (4.0 × 105–1.0 × 107) (n = 12)
rs12979860 T/C 38 (41.3) 1.0 × 104 (1.6 × 103–1.3 × 105) NS 1.0 × 107 (2.8 × 106–1.0 × 107) (n = 37) NS
C/T C/C 41 (44.6) 1.2 × 104 (2.1 × 103–8.2 × 104) (n = 39) 8.6 × 106 (8.4 × 105–1.0 × 107)
CCL2 G/G 3 (3.3) 2.4 × 103 (0–9.4 × 103) 1.0 × 107 (9.2 × 106–1.0 × 107)
rs1024611 G/A 39 (42.4) 1.7 × 104 (1.7 × 103–5.8 × 104) (n = 38) NS 5.5 × 106 (5.2 × 105–1.0 × 107) (n = 37) NS
A/G A/A 50 (54.3) 8.9 × 103 (2.4 × 103–1.4 × 105) (n = 48) 8.5 × 106 (1.6 × 106–1.0 × 107)
DC-SIGN G/G 35 (38.0) 1.2 × 104 (1.4 × 103–1.2 × 105) (n = 32) 1.0 × 107 (1.3 × 106–1.0 × 107)
rs735240 G/A 37 (40.2) 1.1 × 104 (2.4 × 103–1.7 × 105) NS 7.6 × 106 (1.1 × 106–1.0 × 107) (n = 36) NS
A/G A/A 20 (21.7) 7.4 × 103 (1.7 × 103–3.5 × 104) 5.5 × 106 (5.0 × 105–1.0 × 107) (n = 19)
TLR2 G/G 82 (89.1) 1.2 × 104 (2.1 × 103–1.1 × 105) (n = 79) 7.8 × 106 (1.2 × 106–1.0 × 107) (n = 80)
rs5743708 G/A 10 (10.9) 4.3 × 103 (1.6 × 103–3.8 × 104) NS 1.0 × 107 (1.0 × 106–1.0 × 107) NS
A/G A/A 0 (0) NA NA
TLR4 T/T 0 (0) NA NA
rs4986791 T/C 9 (9.8) 2.0 × 104 (2.8 × 103–9.8 × 104) NS 1.0 × 107 (1.7 × 106–1.0 × 107) NS
C/T C/C 83 (90.2) 1.0 × 104 (2.0 × 103–1.0 × 105) (n = 80) 8.6 × 106 (1.1 × 106–1.0 × 107) (n = 81)
TLR9 T/T 30 (32.6) 2.4 × 104 (3.6 × 103–2.6 × 105) (n = 29) 1.0 × 107 (1.7 × 106–1.0 × 107) (n = 29)
rs352140 T/C 47 (51.1) 8.4 × 103 (2.1 × 103–5.7 × 104) (n = 45) NS 5.7 × 106 (8.4 × 105–1.0 × 107) (n = 46) NS
C/T C/C 15 (16.3) 7.9 × 103 (1.1 × 103–3.7 × 104) 1.0 × 107 (1.6 × 106–1.0 × 107)

Association between genotypes of examined polymorphisms and viremia and viruriain asymptomaticneonates with cCMV infection

Asymptomatic infants with cCMV infection(n = 19)
SNP, rs number

SNP database (dbSNP) reference number (ID number).

, alleles		 Genotype n = 19 n (%) HCMV DNA (copies/mL) in blood p-value

p-value – for comparison between genotypes and viremia.

 HCMV DNA (copies/mL) in urine p-value

p-value – for comparison between genotypes and viruria.
IL1B A/A 2 (10.53) 1.4 × 104 (9.9 × 102–2.7 × 104) 5.8 ×106 (2.8 × 106–8.8 × 106)
rs16944 A/G 10 (52.63) 1.8 × 103 (3.1 × 102–4.0 × 103) NS 2.7 ×105 (4.0 × 104–3.2 × 106) NS
G/A G/G 7 (36.84) 2.0 × 103 (1.2 × 103–2.1 × 103) (n = 5) 6.9 ×105 (1.4 × 105–6.2 × 106)
IL12B T/T 14 (73.68) 1.8 × 103 (1.0 × 103–1.4 × 104) (n = 12) 4.9 × 105 (1.4 × 105–4.6 × 106)
rs3212227 T/G 4 (21.05) 2.0 × 103 (0–3.8 × 105) NS 1.1 × 107 (1.0 × 106–4.0 × 107) NS
G/T G/G 1 (5.26) 2.1 × 103 (2.1 × 103–2.1 × 103) 1.7 × 105 (1.7 × 105–1.7 × 105)
IL28B T/T 2 (10.53) 1.9 × 103 (1.2 × 103–2.7 × 103) 2.7 × 105 (2.5 × 105–2.9 × 105)
rs12979860 T/C 6 (31.58) 1.1 × 103 (0–1.6 × 103) NS 2.4× 106 (1.7 × 105–6.2 × 106) NS
C/T C/C 11 (57.89) 2.1 × 103 (2.0 × 103–2.7 × 104) (n = 9) 6.9× 105 (4.0 × 104–6.1 × 106)
CCL2 G/G 0 (0.00) NA NA
rs1024611 G/A 9 (47.37) 2.4 × 103 (9.5 × 102–1.5 × 104) (n = 8) NS 2.5× 105 (1.4 × 105–3.2 × 106) NS
A/G A/A 10 (52.63) 1.2 × 103 (9.9 × 102–2.1 × 103) (n = 9) 1.7× 106 (1.7 × 105–6.1 × 106)
DC-SIGN G/G 7 (36.84) 3.1 × 102 (0–1.2 × 103) (n = 5) 2.9× 105 (3.3 × 103–2.0 × 107)
rs735240 G/A 6 (31.58) 2.0 × 103 (1.6 × 103–2.7 × 104) NS 2.6× 106 (1.7 × 105–6.2 × 106) NS
A/G A/A 6 (31.58) 2.4 × 103 (9.9 × 102–3.2 × 104) 1.1× 106 (4.0 × 104–2.8 × 106)
TLR2 G/G 17 (89.47) 2.1 × 103 (3.1 × 102–2.7 × 104)(n = 15) 2.0× 106 (1.4 × 105–6.1 × 106)
rs5743708 G/A 2 (10.53) 1.4 × 103 (1.2 × 103–1.6 × 103) NS 2.3× 105 (1.7 × 105–2.9 × 105) NS
A/G A/A 0 (0.00) NA NA
TLR4 T/T 0 (0.00) NA NA
rs4986791 T/C 2 (10.53) 2.0 × 103 (0–4.0 × 103) NS 1.0 × 107 (0–2.0 × 107) NS
C/T C/C 17 (89.47) 2.0 × 103 (9.9 × 102–2.7 × 104)(n = 15) 6.9× 105 (1.7 × 105–4.6 × 106)
TLR9 T/T 7 (36.84) 1.4 × 103 (0–4.0 × 103) (n = 6) 2.9× 105 (1.4 × 105–2.0 × 107)
rs352140 T/C 9 (47.37) 2.1 × 103 (1.6 × 103–1.7 × 104)(n = 8) NS 6.9× 105 (1.7 × 105–4.6 × 106) NS
C/T C/C 3 (15.79) 3.1× 102 (0–2.7 × 104) 3.2× 106 (0–8.8 × 106)

Association between alleles of examined polymorphisms (SNPs) and viremia and viruria in newborn infants with cCMV infection

Newborn infants with cCMV infection (N = 92)
SNP, rs number

SNP database (dbSNP) reference number (ID number).

, alleles		 Allele n, (%) HCMV DNA (copies/mL) in blood p-value

p-value – for comparison between alleles and viremia.

 HCMV DNA (copies/mL) in urine p-value

p-value – for comparison between alleles and viruria.
IL1B G 121 (65.76) 1.2 × 104 (2.0 × 103–1.7 × 105) (n = 116) 1.0 ×107 (1.1 × 106–1.0 × 107) (n = 118)
rs16944 NS NS
G/A A 63 (34.24) 7.6 × 103 (2.4 × 103–3.8 × 104) (n = 62) 6.8 ×106 (1.1 × 106–1.0 × 107) (n = 62)
IL12B T 142 (77.17) 1.6 × 104 (2.0 × 103–1.3 × 105) (n = 136) 7.0 ×106 (1.0 × 106–1.0 × 107) (n = 139)
rs3212227 NS
G/T G 42 (22.83) 4.8 × 103 (2.1 × 103–3.0 × 104) 1.0 ×107 (1.7 × 106–1.0 × 107) (n = 41) NS
IL28B C 120 (65.22) 1.1 × 104 (2.1 × 103–9.0 × 104) (n = 116) 8.8 ×106 (1.0 × 106–1.0 × 107) (n = 119)
rs12979860 NS NS
C/T T 64 (34.78) 8.1 × 103 (1.6 × 103–1.3 × 105) (n = 62) 7.0 ×106 (1.3 × 106–1.0 × 107) (n = 61)
CCL2 A 139 (75.54) 1.1 × 104 (2.1 × 103–1.3 × 105) (n = 134) 7.0 ×106 (1.1 × 106–1.0 × 107) (n = 137)
rs1024611 NS NS
A/G G 45 (24.46) 1.0 × 104 (1.6 × 103–4.9 × 104) (n = 44) 9.2 ×106 (8.4 × 105–1.0 × 107) (n = 43)
DC-SIGN G 107 (58.15) 1.1 × 104 (2.0 × 103–1.3 × 105) (n = 101) 9.6 ×106 (1.3 × 106–1.0 × 107) (n = 106)
rs735240 NS NS
A/G A 77 (41.85) 9.4 × 103 (2.1 × 103–5.7 × 104) 6.1 ×106 (1.0 × 106–1.0 × 107) (n = 74)
TLR2 G 174 (94.57) 1.1 × 104 (2.1 × 103–1.0 × 105) (n = 168) 8.6 ×106 (1.1 × 106–1.0 × 107) (n = 170)
rs5743708 NS NS
A/G A 10 (5.43) 4.3 × 103 (1.6 × 103–3.8 × 104) 1.0 ×107 (1.0 × 106–1.0 × 107)
TLR4 C 175 (95.11) 1.0 × 104 (2.1 × 103–9.8 × 104) (n = 169) 8.6 ×106 (1.1 × 106–1.0 × 107) (n = 171)
rs4986791 NS NS
C/T T 9 (4.89) 2.0 × 104 (2.8 × 103–9.8 × 104) 1.0 ×107 (1.7 × 106–1.0 × 107)
TLR9 T 107 (58.15) 1.7 × 103 (2.1 × 103–1.5 × 105) (n = 103) 9.2 ×106 (1.1 × 106–1.0 × 107) (n = 104)
rs352140 NS NS
C/T C 77 (41.85) 7.9 × 103 (1.7 × 103–5.1 × 104) (n = 75) 7.8 ×106 (1.0 × 106–1.0 × 107) (n = 76)

Distribution of genotypes of examined SNPs in the study population of cCMV-infected infants and subgroups of symptomatic and asymptomatic newborn infants

SNP, rs number

SNP database (dbSNP) reference number (ID number).

, alleles		 Genotype cCMV-infected neonates(n = 92) Symptomatic neonates (n = 73) Asymptomatic neonates (n = 19) p-value

p-value for comparison between frequencies of genotypes and subgroups of symptomatic and asymptomatic newborn infants.
Genotype frequencies, n (%)
IL1B A/A 7 (7.61) 5 (6.85) 2 (10.53)
rs16944 A/G 49 (53.26) 39 (53.42) 10 (52.63) NS
G/A G/G 36 (39.13) 29 (39.73) 7 (36.84)
IL12B T/T 57 (61.96) 43 (58.90) 14 (73.68)
rs3212227 T/G 28 (30.43) 24 (32.88) 4 (21.05) NS
G/T G/G 7 (7.61) 6 (8.22) 1 (5.26)
IL28B T/T 13 (14.13) 11 (15.07) 2 (10.53)
rs12979860 T/C 38 (41.30) 32 (43.84) 6 (31.58) NS
C/T C/C 41 (44.57) 30 (41.09) 11 (57.89)
CCL2 G/G 3 (3.26) 3 (4.11) 0 (0.00)
rs1024611 G/A 39 (42.39) 30 (41.10) 9 (47.37) NS
A/G A/A 50 (54.35) 40 (54.79) 10 (52.63)
DC-SIGN G/G 35 (38.04) 28 (38.36) 7 (36.84)
rs735240 G/A 37 (40.22) 31 (42.47) 6 (31.58) NS
A/G A/A 20 (21.74) 14 (19.17) 6 (31.58)
TLR2 G/G 82 (89.13) 65 (89.04) 17 (89.47)
rs5743708 G/A 10 (10.87) 8 (10.96) 2 (10.53) NS
A/G A/A 0 (0.00) 0 (0.00) 0 (0.00)
TLR4 T/T 0 (0.00) 0 (0.00) 0 (0.00)
rs4986791 T/C 9 (9.78) 7 (9.59) 2 (10.53) NS
C/T C/C 83 (90.22) 66 (90.41) 17 (89.47)
TLR9 T/T 30 (32.61) 23 (31.51) 7 (36.84)
rs352140 T/C 47 (51.09) 38 (52.05) 9 (47.37) NS
C/T C/C 15 (16.30) 12 (16.44) 3 (15.79)

Association between genotypes of examined polymorphisms and viremia and viruria in symptomatic infants with cCMV infection

Symptomatic infants with cCMV infection (n = 73)
SNP rs number

SNP database (dbSNP) reference number (ID number).

 alleles		 Genotype n = 73 n (%) HCMV DNA (copies/mL) in blood p-value

p-value – for comparison between genotypes and viremia.

 HCMV DNA (copies/mL) in urine p-value

p-value – for comparison between genotypes and viruria.
A/A 5 (6.85) 6.3 × 103 (5.4 × 103–1.1 × 104) 6.5 × 106 (5.4 × 106–1.0 × 107)
IL1B
rs16944 A/G 39 (53.42) 1.9 × 104 (3.6 × 103–1.1 × 105) (n = 38) NS 1.0 × 107 (1.1 × 106–1.0 × 107) (n = 38) NS
G/A
G/G 29 (39.73) 3.0 × 104 (2.8 × 103–2.1 × 105) 1.0 × 107 (3.7 × 106–1.0 × 107) (n = 28)
T/T 43 (58.90) 3.9 × 104 (5.4 × 103–1.9 × 105) (n = 42) 1.0 × 107 (1.3 × 106–1.0 × 107) (n = 42)
IL12B
rs3212227 T/G 24 (32.88) 9.4 × 103 (2.4 × 103–4.7 × 104) NS 1.0 × 107 (9.2 × 106–1.0 × 107) (n = 23) NS
G/T
G/G 6 (8.22) 7.9 × 103 (2.4 × 103–3.0 × 104) 2.3 × 106 (1.6 × 106–1.0 × 107)
T/T 11 (15.07) 2.2 × 104 (3.3 × 103–2.1 × 105) (n = 10) 7.0 × 106 (1.3 × 106–1.0 × 107) (n = 10)
IL28B
rs12979860 T/C 32 (43.84) 1.9 × 104 (3.2 × 103–1.4 × 105) NS 1.0 × 107 (3.5 × 106–1.0 × 107) (n = 31) NS
C/T
C/C 30 (41.09) 1.6 × 104 (3.9 × 103–1.1 × 105) 1.0 × 107 (1.4 × 106–1.0 × 107)
G/G 3 (4.11) 2.4 × 103 (0–9.4 × 103) 1.0 × 107 (9.2 × 106–1.0 × 107)
CCL2
rs1024611 G/A 30 (41.10) 2.9 × 104 (3.6 × 103–1.1 × 105) NS 1.0 × 107 (1.0 × 106–1.0 × 107) (n = 28) NS
A/G
A/A 40 (54.79) 1.2 × 104 (3.9 × 103–2.0 × 105) (n = 39) 1.0 × 107 (3.7 × 106–1.0 × 107)
G/G 28 (38.36) 2.9 × 104 (4.3 × 103–1.5 × 105) (n = 27) 1.0 × 107 (4.2 × 106–1.0 × 107)
DC-SIGN
rs735240 G/A 31 (42.47) 1.2 × 104 (3.6 × 103–1.9 × 105) NS 1.0 × 107 (1.4 × 106–1.0 × 107) (n = 30) NS
A/G
A/A 14(19.17) 1.3 × 104 (2.8 × 103–3.8 × 104) 1.0 × 107 (1.7 × 106–1.0 × 107) (n = 13)
G/G 65 (89.04) 1.9 × 104 (3.7 × 103–1.7 × 105) (n = 64) 1.0 × 107 (1.7 × 106–1.0 × 107) (n = 63)
TLR2
rs5743708 G/A 8 (10.96) 5.0 × 103 (2.8 × 103–8.4 × 104) NS 1.0 × 107 (5.5 × 106–1.0 × 107) NS
A/G
A/A 0 (0.00) NA NA
T/T 0 (0.00) NA NA
TLR4
rs4986791 T/C 7 (9.59) 3.8 × 104 (2.8 × 103–1.1 × 105) NS 1.0 × 107 (1.7 × 106–1.0 × 107) NS
C/T
C/C 66 (9×0.41) 1.4 × 104 (3.8 × 103–1.5 × 105) (n = 65) 1.0 × 107 (1.7 × 106–1.0 × 107) (n = 64)
TLR9 T/T 23 (31.51) 2.9 × 104 (5.3 × 103–2.6 × 105) 1.0 × 107 (5.5 × 106–1.0 × 107) (n = 22)
rs352140 T/C 38 (52.05) 1.4 × 104 (2.8 × 103–8.2 × 104) (n = 37) NS 1.0 × 107 (1.1 × 106–1.0 × 107) (n = 37) NS
C/T C/C 12 (16.44) 8.6 × 103 (3.0 × 103–4.7 × 104) 1.0 × 107 (4.3 × 106–1.0 × 107)

Association between alleles of examined polymorphisms (SNPs) and viremia and viruria in symptomatic newborn infants with cCMV infection.

Symptomatic newborn infants with cCMV infection (N = 73)
SNP, rs number

SNP database (dbSNP) reference number (ID number).

, alleles		 Allele Alleles, n (%) HCMV (copies/DNA mL) in blood p-value

p-value – for comparison between alleles and viremia.

 HCMV DNA (copies/mL) in urine p-value

p-value – for comparison between alleles and viruria.
IL1B G 97 (66.44) 2.2 × 104 (3.0 × 103–1.9 × 105) (n = 96) 1.0 × 107 (2.9 × 106–1.0 × 107) (n = 94)
rs16944 NS NS
G/A A 49 (33.56) 1.1 × 104 (3.8 × 103–5.4 × 104) (n = 48) 1.0 × 107 (1.5 × 106–1.0 × 107) (n = 48)
IL12B T 110 (75.34) 2.6 × 104 (4.7 × 103–1.8 × 105) 1.0 × 107 (1.4 × 106–1.0 × 107)
rs3212227 (n = 108) 0.037 (n = 107) NS
G/T G 36 (24.66) 9.4 × 103 (2.4 × 103–3.4 × 104) 1.0 × 107 (1.7 × 106–1.0 × 107) (n = 35)
IL28B C 92 (63.01) 1.7 × 104 (3.8 × 103–1.2 × 105) 1.0 × 107 (1.7 × 106–1.0 × 107) (n = 91)
rs12979860 NS NS
C/T T 54 (36.99) 1.9 × 104 (3.3 × 103–1.8 × 105) (n = 52) 1.0 × 107 (1.6 × 106–1.0 × 107) (n = 51)
CCL2 A 110 (75.34) 1.9 × 104 (3.9 × 103–1.7 × 105) 1.0 × 107 (1.7 × 106–1.0 × 107)
rs1024611 (n = 108) NS (n = 108) NS
A/G G 36 (24.66) 1.6 × 104 (2.0 × 103–5.4 × 104) 1.0 × 107 (1.4 × 106–1.0 × 107) (n = 34)
DC-SIGN G 87 (59.59) 2.8 × 104 (3.9 × 103–1.5 × 105) (n = 85) 1.0 × 107 (2.9 × 106–1.0 × 107) (n = 86)
rs735240 NS NS
A/G A 59 (40.41) 1.2 × 104 (3.3 × 103–8.2 × 104) 1.0 × 107 (1.5 × 106–1.0 × 107) (n = 56)
TLR2 G 138 (94.52) 1.7 × 104 (3.7 × 103–1.5 × 105) 1.0 × 107 (1.7 × 106–1.0 × 107)
rs5743708 (n = 136) NS (n = 134) NS
A/G A 8 (5.48) 5.0 × 103 (2.8 × 103–8.4 × 104) 1.0 × 107 (5.5 × 106–1.0 × 107)
TLR4 rs4986791 C 139 (95.21) 1.7 × 104 (3.8 × 103–1.5 × 105) (n = 137) NS 1.0 × 107 (1.7 × 106–1.0 × 107) (n = 135) NS
C/T T 7 (4.79) 3.8 × 104 (2.8 × 103–1.1 × 105) 1.0 × 107 (1.7 × 106–1.0 × 107)
TLR9 T 84 (57.53) 2.8 × 104 (3.8 × 103–1.9 × 105) (n = 83) 1.0 × 107 (2.9 × 106–1.0 × 107) (n = 81)
rs352140 NS NS
C/T C 62 (42.47) 9.4 × 103 (2.8 × 103–5.8 × 104) (n = 61) 1.0 × 107 (1.6 × 106–1.0 × 107) (n = 61)
eISSN:
2719-535X
Langue:
Anglais
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Volume Open
Sujets de la revue:
Medicine, Clinical Medicine, Pediatrics and Juvenile Medicine, Paediatric Haematology and Oncology, Public Health
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