<abstract xmlns="http://www.w3.org/1999/xhtml"><p>The present study aimed to evaluate the effectiveness of low-frequency ultrasounds applied to eliminate <italic>Campylobacter</italic> spp. from water. The strains used in this research were isolated from water contaminated with sewage. <italic>Campylobacter coli</italic> alone was detected in the samples and used for further research. The reference strain <italic>C. coli</italic> ATCC 33559 was simultaneously tested. The isolate was exposed to ultrasounds at frequencies of 37 kHz and 80 kHz in a continuous operation device with ultrapure deionized water. After 5 min of sonication, the count of <italic>C. coli</italic> decreased by 5.78% (37 kHz) and 6.27% (80 kHz), whereas the temperature increased by 3°C (37 kHz), and 6°C (80 kHz). After 30 min of sonication, the death rates of bacterial cells were 40.15% (37 kHz) and 55.10% (80 kHz), whereas the temperature reached the maximum values of 36°C (37 kHz), and 39°C (80 kHz). Sonication at the frequency of 80 kHz reduced the bacterial count from 6.86 log CFU/ml to 3.08 log CFU/ml, whereas the frequency of 37 kHz reduced the bacterial count from 6.75 log CFU/ml to 4.04 log CFU/ml. Despite significant differences (<italic>p</italic> &lt; 0.05) in the number of <italic>C. coli</italic> cells, the cell death rate remained at the same level.<fig id="j_pjm-2021-032_fig_001" position="float" fig-type="figure"><graphic xmlns:xlink="http://www.w3.org/1999/xlink" xlink:href="graphic/j_pjm-2021-032_fig_001.jpg"></graphic></fig></p></abstract>

The present study aimed to evaluate the effectiveness of low-frequency ultrasounds applied to eliminate Campylobacter spp. from water. The strains used in this research were isolated from water contaminated with sewage. Campylobacter coli alone was detected in the samples and used for further research. The reference strain C. coli ATCC 33559 was simultaneously tested. The isolate was exposed to ultrasounds at frequencies of 37 kHz and 80 kHz in a continuous operation device with ultrapure deionized water. After 5 min of sonication, the count of C. coli decreased by 5.78% (37 kHz) and 6.27% (80 kHz), whereas the temperature increased by 3°C (37 kHz), and 6°C (80 kHz). After 30 min of sonication, the death rates of bacterial cells were 40.15% (37 kHz) and 55.10% (80 kHz), whereas the temperature reached the maximum values of 36°C (37 kHz), and 39°C (80 kHz). Sonication at the frequency of 80 kHz reduced the bacterial count from 6.86 log CFU/ml to 3.08 log CFU/ml, whereas the frequency of 37 kHz reduced the bacterial count from 6.75 log CFU/ml to 4.04 log CFU/ml. Despite significant differences (p < 0.05) in the number of C. coli cells, the cell death rate remained at the same level.

Use of Ultrasounds to Reduce the Count of <italic>Campylobacter coli</italic> in Water

Polish Journal of Microbiology

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Use of Ultrasounds to Reduce the Count of  in Water

The present study aimed to evaluate the effectiveness of low-frequency ultrasounds applied to eliminate Campylobacter spp. from water. The strains used in this...

Time[min]	37 kHz					80 kHz
Time[min]	C. coli	SD	C. coliATCC 33559	SD	Temperature[°C]	C. coli	SD	C. coliATCC 33559	SD	Temperature[°C]
0	6.75	± 0.7	6.78	± 1.0	20	6.86	± 0.9	6.83	± 1.0	21
5	6.36	± 0.5	6.38	± 0.8	23	6.43	± 0.6	6.38	± 0.8	27
10	5.25	± 0.5	5.18	± 0.4	24	4.18	± 0.5	4.11	± 0.4	31
15	5.00	± 0.4	5.04	± 0.7	28	3.95	± 0.7	3.84	± 0.5	33
20	4.90	± 0.6	4.84	± 0.6	30	3.84	± 0.7	3.60	± 0.4	36
25	4.48	± 0.4	4.30	± 0.5	34	3.48	± 0.4	3.00	± 0.3	37
30	4.04	± 0.3	3.95	± 0.4	36	3.08	± 0.5	2.48	± 0.5	39

Use of Ultrasounds to Reduce the Count of Campylobacter coli in Water

Article Category: original-paper

Publié en ligne: 17 sept. 2021

Pages: 339 - 343

Reçu: 31 mars 2021

Accepté: 14 juil. 2021

DOI: https://doi.org/10.33073/pjm-2021-032

Mots clés
sonication, sewage

© 2021 Marek Selwet published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Influence of the sonication process on temperature changes and the number of Campylobacter coli [log CFU/ml].

Use of Ultrasounds to Reduce the Count of Campylobacter coli in Water

Article Category: original-paper

Publié en ligne: 17 sept. 2021

Pages: 339 - 343

Reçu: 31 mars 2021

Accepté: 14 juil. 2021

DOI: https://doi.org/10.33073/pjm-2021-032

Mots cléssonication, sewage

© 2021 Marek Selwet published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Influence of the sonication process on temperature changes and the number of Campylobacter coli [log CFU/ml].

Mots clés
sonication, sewage