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Isolated Phosphate-Solubilizing Soil Bacteria Promotes In vitro Growth of Solanum tuberosum L.

GUSTAVO YAÑEZ-OCAMPO
GUSTAVO YAÑEZ-OCAMPO
, 
MARTHA E. MORA-HERRERA
MARTHA E. MORA-HERRERA
, 
ARNOLDO WONG-VILLARREAL
ARNOLDO WONG-VILLARREAL
, 
DENISSE M. DE LA PAZ-OSORIO
DENISSE M. DE LA PAZ-OSORIO
, 
NADIA DE LA PORTILLA-LÓPEZ
NADIA DE LA PORTILLA-LÓPEZ
, 
JORGE LUGO
JORGE LUGO
, 
ROCIO VACA-PAULÍN
ROCIO VACA-PAULÍN
 et 
PEDRO DEL ÁGUILA
PEDRO DEL ÁGUILA
   | 08 sept. 2020
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Article Category: original-paper
Publié en ligne: 08 sept. 2020
Pages: 357 - 365
Reçu: 04 juin 2020
Accepté: 15 août 2020
DOI: https://doi.org/10.33073/pjm-2020-039
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© 2020 Gustavo Yañez-Ocampo et al., published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Fig. 1.

Phosphate solubilization assay, measured by halo formation by four bacterial strains cultured on PVK agar. The lines above each bar indicate standard deviation. The different letters above the bars denote statistical differences (LSD test (p < 0.001)).
Phosphate solubilization assay, measured by halo formation by four bacterial strains cultured on PVK agar. The lines above each bar indicate standard deviation. The different letters above the bars denote statistical differences (LSD test (p < 0.001)).

Fig. 2.

Kinetics of the bacterial growth on PVK liquid medium.a) the bacterial growth measured as optical density at a wavelength of 600 nm, b) the orthophosphates released measured with molybdenum blue method, and c) the acidification evidence (lower pH values) in PVK liquid medium. The lines shown above or below each point indicate the standard deviation.
Kinetics of the bacterial growth on PVK liquid medium.a) the bacterial growth measured as optical density at a wavelength of 600 nm, b) the orthophosphates released measured with molybdenum blue method, and c) the acidification evidence (lower pH values) in PVK liquid medium. The lines shown above or below each point indicate the standard deviation.

Fig. 3.

Phylogenetic tree of A3 bacterial strain identified by 16 S rDNA molecular analysis.
Phylogenetic tree of A3 bacterial strain identified by 16 S rDNA molecular analysis.

Fig. 4.

Development of adventitious roots in potato microplants (Solanum tuberosum L.) cv. Citlali, inoculated with PSB strain A3 and cultured for 28 d ± 2 d.
Development of adventitious roots in potato microplants (Solanum tuberosum L.) cv. Citlali, inoculated with PSB strain A3 and cultured for 28 d ± 2 d.

Potato microplants (Solanum tuberosum L.) cv. Citlali grown for 28 ± 2 d in vitro in presence/absence of PSB strain A3.

TreatmentsStem length (cm)Root length (cm)Fresh weight (g)
Bacterial strain A3 added  10.043 ± 3.252*  2.943 ± 1.634*  0.573 ± 0.296*
Without added bacterial strain A35.6 ± 2.0264.297 ± 3.1360.174 ± 0.161

Growth evaluation of potato microplants cv. Citlali with different P sources and sucrose concentrations.

Variable
Phosphate (A)Sucrose g/l (B)Interaction
KH2PO4 (T0, T1)Ca3(PO4)2 (T4, T5)  10 (T1, T5)  30 (T0, T4)AB
1*6.081 ± 0.154¤b6.801 ± 0.155a6.122 ± 0.153b6.760 ± 0.156aNo
215.099 ± 0.640NS16.180 ± 0.636NS14.335 ± 0.632b16.944 ± 0.644aNo
30.279 ± 0.093NS0.334 ± 0.094NS0.240 ± 0.0195b0.373 ± 0.0198aPresent
eISSN:
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Langue:
Anglais
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Sujets de la revue:
Life Sciences, Microbiology and Virology
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