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Chemical Composition of Aspergillus creber Extract and Evaluation of its Antimicrobial and Antioxidant Activities

AFAF SAKHRI
AFAF SAKHRI
, 
NOREDDINE KACEM CHAOUCHE
NOREDDINE KACEM CHAOUCHE
, 
MARIA ROSARIA CATANIA
MARIA ROSARIA CATANIA
, 
ALBERTO RITIENI
ALBERTO RITIENI
 et 
ANTONELLO SANTINI
ANTONELLO SANTINI
   | 03 sept. 2019
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Article Category: original-paper
Publié en ligne: 03 sept. 2019
Pages: 309 - 316
Reçu: 18 mars 2019
Accepté: 07 mai 2019
DOI: https://doi.org/10.33073/pjm-2019-033
Mots clés
© 2019 Anton Pyskun et al., published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Fig. 1.

Total Ion Current chromatogram of Aspergillus creber extract obtained with UHPLC-MS/MS. The retention times of the peaks (1–10) and their corresponding molecules names are presented in Table I.
Total Ion Current chromatogram of Aspergillus creber extract obtained with UHPLC-MS/MS. The retention times of the peaks (1–10) and their corresponding molecules names are presented in Table I.

Fig. 2.

Free radical-scavenging activities of Aspergillus creber extract and ascorbic acid (400 μg/ml) measured (a), in DPPH assay and (b), in ABTS assay.
Free radical-scavenging activities of Aspergillus creber extract and ascorbic acid (400 μg/ml) measured (a), in DPPH assay and (b), in ABTS assay.

Metabolites of Aspergillus creber as determined by UHPLC-MS/MS. The metabolites were tentatively identified using molecular mass data, MS/MS analysis and references.

Peak No.Putative compound nameAdductionMeasured mass (m/z)Productions (m/z)tRa (min)Refb
1Asperlactone[M+H] +185.08081141.05444, 113.059481.73Vishwanath et al. 2009
2NI[M+Na] +211.06121195.03455, 133.028161.78-
3NI[M+H] +308.10992280.95587, 145.101043.38-
4NI[M+H] +327.04734309.18594, 191.153964.28-
5Emodin[M+H]-269.04590ND5.42Sulyok et al. 2007; Lehner et al. 2011; Micheluz et al. 2016
6NI[M+H]+251.09145233.09526, 204.093195.49-
7Sterigmatocystin[M+H]+325.07053ND6.62Lehner et al. 2011; Micheluz et al. 2016
8NI[M+Na] +423.25041405.27911, 239.148217.27-
9Deoxybrevianamide E[M+H]+352.20352ND8.04Lehner et al. 2011; Micheluz et al. 2016
10Norsolorinic acid[M+H]-369.09892ND8.24Micheluz et al. 2016

Antimicrobial activity and minimum inhibitory concentration (MIC) of ethyl acetate extract of Aspergillus creber against human pathogens.

Zone of inhibition (mm)aMIC of ethyl acetate extract (mg/ml)
Ethyl acetate extractPositive controlb
Bacteria
Escherichia coli023.4 ± 0.3-
Klebsiella pneumoniae10.0 ± 0.325.0 ± 0.42.5
Pseudomonas aeruginosa020.8 ± 0.4-
Salmonella Typhi8.5 ± 0.621.5 ± 0.35
SABLc12.8 ± 0.319.8 ± 0.20.625
MRSEd14.0 ± 0.219.2 ± 0.20.625
Yeasts
Candida albicans20.6 ± 0.822.8 ± 0.40.325
Candida glabrata13.0 ± 0.325.7 ± 0.51.25

Antioxidant activities of Aspergillus creber ethyl acetate extract by DPPH and ABTS assays and its total phenolic content.

ActivityExtractAscorbic acida
DPPHa89.28 ± 0.3291.39 ± 0.39
ABTSa92.93 ± 0.3093.03 ± 0.45
Total phenolic content (mg GAE/g)85.76 ± 0.96-
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Langue:
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Sujets de la revue:
Life Sciences, Microbiology and Virology
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