<abstract xmlns="http://www.w3.org/1999/xhtml">

<p>Retrotransposon movements are considered to be an important factor in evolutionary processes and speciation as well as a source of genetic variation. In order to analyze genetic diversity and population structure in Tunisian pistachio species, nine inter-retrotransposon amplified polymorphism (IRAP) markers were used. As a result, eighty-six amplicons were produced among which 98.15 % were polymorphic. Mean numbers of the effective number of alleles (Ne), Shannon’s information index (I) and Nei’s genetic diversity (H) were respectively 1.529, 0.478, and 0.310. The average within-population genetic diversity (Hs) was 0.24 and the total diversity (Ht) was 0.3. The Tunisian pistachio populations exhibited high genetic differentiation (Gst =0.275) and gene flow (<italic>Nm</italic> = 1.888). The Analysis of Molecular Variance (AMOVA) indicated that variation was very high within populations (83 %). Phylogenetic tree using neighbor- joining (NJ) method and Principal Coordinates Analysis (PCoA) depicted that groupings of Tunisian varieties were made independently of the sex of the trees, but depending on their geographical origin and their breeding status. The modelbased Bayesian clustering (STRUCTURE) confirmed these observations. The inter-retrotransposons amplification polymorphism markers were significantly informative at the interspecific level. Findings reported in our study will be essential toward breeding for new pistachio genotypes with developed chemical and horticultural features.</p>
</abstract>

Retrotransposon movements are considered to be an important factor in evolutionary processes and speciation as well as a source of genetic variation. In order to analyze genetic diversity and population structure in Tunisian pistachio species, nine inter-retrotransposon amplified polymorphism (IRAP) markers were used. As a result, eighty-six amplicons were produced among which 98.15 % were polymorphic. Mean numbers of the effective number of alleles (Ne), Shannon’s information index (I) and Nei’s genetic diversity (H) were respectively 1.529, 0.478, and 0.310. The average within-population genetic diversity (Hs) was 0.24 and the total diversity (Ht) was 0.3. The Tunisian pistachio populations exhibited high genetic differentiation (Gst =0.275) and gene flow (Nm = 1.888). The Analysis of Molecular Variance (AMOVA) indicated that variation was very high within populations (83 %). Phylogenetic tree using neighbor- joining (NJ) method and Principal Coordinates Analysis (PCoA) depicted that groupings of Tunisian varieties were made independently of the sex of the trees, but depending on their geographical origin and their breeding status. The modelbased Bayesian clustering (STRUCTURE) confirmed these observations. The inter-retrotransposons amplification polymorphism markers were significantly informative at the interspecific level. Findings reported in our study will be essential toward breeding for new pistachio genotypes with developed chemical and horticultural features.

Retrotransposon-based markers revealed a repartition depending on geographical origin and breeding status of Tunisian pistachio species

Laboratory of Genetics, Biodiversity and Bioresources Valorisation (LR11ES41)

Laboratory of Molecular Genetics, Immunology and Biotechnology, Faculty of Sciences of Tunis

Silvae Genetica

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Retrotransposon movements are considered to be an important factor in evolutionary processes and speciation as well as a source of genetic variation. In order...

Retrotransposon-based markers revealed a repartition depending on geographical origin and breeding status of Tunisian pistachio species

Publié en ligne: 27 févr. 2022

Pages: 1 - 9

DOI: https://doi.org/10.2478/sg-2022-0001

Mots clés
Pistacia, Retrotransposon, population structure, gene flow, plant breeding

© 2022 Khaled Chatti et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Retrotransposon-based markers revealed a repartition depending on geographical origin and breeding status of Tunisian pistachio species

Publié en ligne: 27 févr. 2022

Pages: 1 - 9

DOI: https://doi.org/10.2478/sg-2022-0001

Mots clésPistacia, Retrotransposon, population structure, gene flow, plant breeding

© 2022 Khaled Chatti et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Mots clés
Pistacia, Retrotransposon, population structure, gene flow, plant breeding