<abstract xmlns="http://www.w3.org/1999/xhtml">

<p>The improvement of Eucalyptus clones plays a crucial role in modern silviculture. This study used a set of 17 microsatellite loci to analyze the genetic diversity and structure of 107 elite clones (80 <italic>E. grandis</italic> and 27 <italic>E. globulus</italic>). All clones were cultivated in Uruguay and were sourced from three different providers. Using the fingerprinting technique, an exclusive molecular profile was assigned for each clone, and the genotyping reaction showed differences between the two species. The cumulative probability of identifying two random individuals that share the same genotype (<italic>PI</italic>) with all 17 loci, was estimated as low for <italic>E. grandis</italic> (1.18×10-15) and <italic>E. globulus</italic> (4.03×10-14). The combined <italic>PIsibs</italic> was (1.05×10-5) and (2.17×10-5) for <italic>E. grandis</italic> and <italic>E. globulus</italic>, respectively. A total of 180 alleles were detected for <italic>E. grandis</italic> and 100 for <italic>E. globulus</italic>. We found a high mean number of alleles per locus (10 for <italic>E. grandis</italic> and 6 for <italic>E. globulus</italic>), and the results for mean polymorphic information content (<italic>PIC</italic> ) were (0.648) and (0.548), respectively. The observed heterozygosity (<italic>H<sub>o</sub></italic>) ranged from 0.216 to 0.838 (mean = 0.509) for <italic>E. grandis</italic> and 0 to 1 (mean = 0.566) for <italic>E. globulus</italic>. Two core sets of seven EST-SSR loci were identified for each species. These markers revealed unambiguous fragment amplification, providing a minimum number of SSRs for effective clonal identification. The genetic structure analysis suggests that the germplasm of the <italic>E. grandis</italic> population is structured in four clusters, while the <italic>E. globulus</italic> population consists of two clusters.</p>
</abstract>

The improvement of Eucalyptus clones plays a crucial role in modern silviculture. This study used a set of 17 microsatellite loci to analyze the genetic diversity and structure of 107 elite clones (80 E. grandis and 27 E. globulus). All clones were cultivated in Uruguay and were sourced from three different providers. Using the fingerprinting technique, an exclusive molecular profile was assigned for each clone, and the genotyping reaction showed differences between the two species. The cumulative probability of identifying two random individuals that share the same genotype (PI) with all 17 loci, was estimated as low for E. grandis (1.18×10-15) and E. globulus (4.03×10-14). The combined PIsibs was (1.05×10-5) and (2.17×10-5) for E. grandis and E. globulus, respectively. A total of 180 alleles were detected for E. grandis and 100 for E. globulus. We found a high mean number of alleles per locus (10 for E. grandis and 6 for E. globulus), and the results for mean polymorphic information content (PIC ) were (0.648) and (0.548), respectively. The observed heterozygosity (Ho) ranged from 0.216 to 0.838 (mean = 0.509) for E. grandis and 0 to 1 (mean = 0.566) for E. globulus. Two core sets of seven EST-SSR loci were identified for each species. These markers revealed unambiguous fragment amplification, providing a minimum number of SSRs for effective clonal identification. The genetic structure analysis suggests that the germplasm of the E. grandis population is structured in four clusters, while the E. globulus population consists of two clusters.

The traceability of <italic>Eucalyptus</italic> clones using molecular markers

Centro Universitario de Tacuarembó (CUT)

Silvae Genetica

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

The traceability of  clones using molecular markers

The improvement of Eucalyptus clones plays a crucial role in modern silviculture. This study used a set of 17 microsatellite loci to analyze the genetic...

The traceability of Eucalyptus clones using molecular markers

Publié en ligne: 26 déc. 2021

Pages: 217 - 225

DOI: https://doi.org/10.2478/sg-2021-0019

Mots clés
Clone certification, clone, genotyping, identity, multiplex, nurseries, traceability

© 2020 Diego Torres-Dini et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

The traceability of Eucalyptus clones using molecular markers

Publié en ligne: 26 déc. 2021

Pages: 217 - 225

DOI: https://doi.org/10.2478/sg-2021-0019

Mots clésClone certification, clone, genotyping, identity, multiplex, nurseries, traceability

© 2020 Diego Torres-Dini et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Mots clés
Clone certification, clone, genotyping, identity, multiplex, nurseries, traceability