Giardia duodenalis in faecal samples from calves in Poland

Giardia duodenalis is the prevalent parasitic protozoan responsible for diarrhoeal disease in humans and animals. This study aimed to determine the prevalence and assemblages of this parasite present in cattle in Poland.

Material and Methods

Faecal samples were collected from 1,602 cattle up to 4 months old, bred on 267 farms located in all 16 voivodeships of Poland. Extraction of DNA was performed using a modified alkaline and a heat lysis method. Molecular detection of β-giardin gene by PCR and sequence analysis were performed.

Results

In 174 of 1,602 (10.9%) tested cattle and in 89 of 267 examined herds (33.3%), G. duodenalis DNA was detected. The highest prevalence of Giardia in cattle was found in the Lubelskie (25%), Mazowieckie (21.3%) and Zachodniopomorskie (20.4%) voivodeships. In other regions, the prevalence did not exceed 10%. The number of Giardia-positive cattle decreased with animal age. The most frequently identified assemblage in cattle was E (89.2%), and A and B were detected more seldom in 9.0% and 1.8% of cattle, respectively. No significant differences in positive results were observed in cattle depending on production purpose.

Conclusion

The study results showed a high prevalence of Giardia in cattle in Poland. The presence of the Giardia A and B assemblages indicates a potential zoonotic threat.

Langue:: Anglais

Périodicité:: 4 fois par an
Sujets de la revue:: Sciences de la vie, Biologie moléculaire, Microbiologie et virologie, Sciences de la vie, autres, Médecine, Médecine vétérinaire

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Giardia duodenalis in faecal samples from calves in Poland

Jacek Sroka

Jacek Zwoliński

Ewa Bilska-Zając

Angelina Wójcik Fatla

Jacek Karamon

Jolanta Małgorzata Zdybel

Weronika Wiktoria Korpysa-Dzirba

Małgorzata Samorek-Pieróg

Weronika Piotrowska

Tomasz Cencek

Publié en ligne: 24 sept. 2025

Reçu: 08 mai 2025

Accepté: 18 sept. 2025

DOI: https://doi.org/10.2478/jvetres-2025-0052

Mots cléscattle, molecular characterisation, PCR

© 2025 Jacek Sroka et al., published by Sciendo

This work is licensed under the Creative Commons Attribution 4.0 International License.

Introduction

Material and Methods

Results

Conclusion

Mots clés
cattle, molecular characterisation, PCR