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Molecular characterisation and antimicrobial resistance of Streptococcus agalactiae isolates from dairy farms in China

Xiaojiao Ma
Xiaojiao Ma
, 
He Chen
He Chen
, 
Fulan Wang
Fulan Wang
, 
Shuai Wang
Shuai Wang
, 
Yating Wu
Yating Wu
, 
Xianlan Ma
Xianlan Ma
, 
Yong Wei
Yong Wei
, 
Wei Shao
Wei Shao
 et 
Yankun Zhao
Yankun Zhao
   | 16 juin 2023
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Publié en ligne: 16 juin 2023
Pages: 161 - 167
Reçu: 23 déc. 2022
Accepté: 25 avr. 2023
DOI: https://doi.org/10.2478/jvetres-2023-0027
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© 2023 Xiaojiao Ma et al., published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Introduction

Streptococcus agalactiae (S. agalactiae) is a pathogen causing bovine mastitis that results in considerable economic losses in the livestock sector. To understand the distribution and drug resistance characteristics of S. agalactiae from dairy cow mastitis cases in China, multilocus sequence typing (MLST) was carried out and the serotypes and drug resistance characteristics of the bacteria in the region were analysed.

Material and Methods

A total of 21 strains of bovine S. agalactiae were characterised based on MLST, molecular serotyping, antimicrobial susceptibility testing, and the presence of drug resistance genes.

Results

The serotypes were mainly Ia and II, accounting for 47.6% and 42.9% of all serotypes, respectively. Five sequence types (STs) were identified through MLST. The ST103 and ST1878 strains were predominant, with rates of 52.4% and 28.6%, respectively. The latter is a novel, previously uncharacterised sequence type. More than 90% of S. agalactiae strains were susceptible to penicillin, oxacillin, cephalothin, ceftiofur, gentamicin, florfenicol and sulfamethoxazole. The bacteria showed high resistance to tetracycline (85.7%), clindamycin (52.1%) and erythromycin (47.6%). Resistant genes were detected by PCR, the result of which showed that 47.6%, 33.3% and 38.1% of isolates carried the tet(M), tet(O) and erm(B) genes, respectively.

Conclusion

The results of this study indicate that S. agalactiae show a high level of antimicrobial resistance. It is necessary to monitor the pathogens of mastitis to prevent the transmission of these bacteria.
eISSN:
2450-8608
Langue:
Anglais
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Sujets de la revue:
Life Sciences, Molecular Biology, Microbiology and Virology, other, Medicine, Veterinary Medicine
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