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Molecular characterisation and antimicrobial resistance of Streptococcus agalactiae isolates from dairy farms in China

Xiaojiao Ma

Xiaojiao Ma

  • College of Animal Science Xinjiang Agriculture UniversityUrumqi, P. R. China
  • Institute of Quality Standards & Testing Technology for Agro-Products, Xinjiang Academy of Agricultural SciencesUrumqi, P. R. China
  • Ministry of Agriculture and Rural Affairs-Laboratory of Quality and Safety Risk Assessment for Agro-ProductsUrumqi, P. R. China
  • Key Laboratory of Agro-Products Quality and Safety of XinjiangUrumqi, P. R. China
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Ma, Xiaojiao
, 
He Chen

He Chen

  • Institute of Quality Standards & Testing Technology for Agro-Products, Xinjiang Academy of Agricultural SciencesUrumqi, P. R. China
  • Ministry of Agriculture and Rural Affairs-Laboratory of Quality and Safety Risk Assessment for Agro-ProductsUrumqi, P. R. China
  • Key Laboratory of Agro-Products Quality and Safety of XinjiangUrumqi, P. R. China
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Chen, He
, 
Fulan Wang

Fulan Wang

  • Institute of Quality Standards & Testing Technology for Agro-Products, Xinjiang Academy of Agricultural SciencesUrumqi, P. R. China
  • Ministry of Agriculture and Rural Affairs-Laboratory of Quality and Safety Risk Assessment for Agro-ProductsUrumqi, P. R. China
  • Key Laboratory of Agro-Products Quality and Safety of XinjiangUrumqi, P. R. China
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Wang, Fulan
, 
Shuai Wang

Shuai Wang

  • Institute of Quality Standards & Testing Technology for Agro-Products, Xinjiang Academy of Agricultural SciencesUrumqi, P. R. China
  • Ministry of Agriculture and Rural Affairs-Laboratory of Quality and Safety Risk Assessment for Agro-ProductsUrumqi, P. R. China
  • Key Laboratory of Agro-Products Quality and Safety of XinjiangUrumqi, P. R. China
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Wang, Shuai
, 
Yating Wu

Yating Wu

  • Institute of Quality Standards & Testing Technology for Agro-Products, Xinjiang Academy of Agricultural SciencesUrumqi, P. R. China
  • Ministry of Agriculture and Rural Affairs-Laboratory of Quality and Safety Risk Assessment for Agro-ProductsUrumqi, P. R. China
  • Key Laboratory of Agro-Products Quality and Safety of XinjiangUrumqi, P. R. China
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Wu, Yating
, 
Xianlan Ma

Xianlan Ma

  • Institute of Quality Standards & Testing Technology for Agro-Products, Xinjiang Academy of Agricultural SciencesUrumqi, P. R. China
  • Ministry of Agriculture and Rural Affairs-Laboratory of Quality and Safety Risk Assessment for Agro-ProductsUrumqi, P. R. China
  • Key Laboratory of Agro-Products Quality and Safety of XinjiangUrumqi, P. R. China
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Ma, Xianlan
, 
Yong Wei

Yong Wei

Xinjiang Tian’ao Animal Husbandry Co., LtdIli, P. R. China
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Wei, Yong
, 
Wei Shao

Wei Shao

College of Animal Science Xinjiang Agriculture UniversityUrumqi, P. R. China
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Shao, Wei
 y 
Yankun Zhao

Yankun Zhao

  • Institute of Quality Standards & Testing Technology for Agro-Products, Xinjiang Academy of Agricultural SciencesUrumqi, P. R. China
  • Ministry of Agriculture and Rural Affairs-Laboratory of Quality and Safety Risk Assessment for Agro-ProductsUrumqi, P. R. China
  • Key Laboratory of Agro-Products Quality and Safety of XinjiangUrumqi, P. R. China
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Zhao, Yankun
  
16 jun 2023
Molecular characterisation and antimicrobial resistance of Streptococcus agalactiae isolates from dairy farms in China's Cover Image
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Publicado en línea: 16 jun 2023
Páginas: 161 - 167
Recibido: 23 dic 2022
Aceptado: 25 abr 2023
DOI: https://doi.org/10.2478/jvetres-2023-0027
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© 2023 Xiaojiao Ma et al., published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Introduction

Streptococcus agalactiae (S. agalactiae) is a pathogen causing bovine mastitis that results in considerable economic losses in the livestock sector. To understand the distribution and drug resistance characteristics of S. agalactiae from dairy cow mastitis cases in China, multilocus sequence typing (MLST) was carried out and the serotypes and drug resistance characteristics of the bacteria in the region were analysed.

Material and Methods

A total of 21 strains of bovine S. agalactiae were characterised based on MLST, molecular serotyping, antimicrobial susceptibility testing, and the presence of drug resistance genes.

Results

The serotypes were mainly Ia and II, accounting for 47.6% and 42.9% of all serotypes, respectively. Five sequence types (STs) were identified through MLST. The ST103 and ST1878 strains were predominant, with rates of 52.4% and 28.6%, respectively. The latter is a novel, previously uncharacterised sequence type. More than 90% of S. agalactiae strains were susceptible to penicillin, oxacillin, cephalothin, ceftiofur, gentamicin, florfenicol and sulfamethoxazole. The bacteria showed high resistance to tetracycline (85.7%), clindamycin (52.1%) and erythromycin (47.6%). Resistant genes were detected by PCR, the result of which showed that 47.6%, 33.3% and 38.1% of isolates carried the tet(M), tet(O) and erm(B) genes, respectively.

Conclusion

The results of this study indicate that S. agalactiae show a high level of antimicrobial resistance. It is necessary to monitor the pathogens of mastitis to prevent the transmission of these bacteria.
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Ciencias de la vida, Biología molecular, Microbiología y virología, Ciencias de la vida, otros, Medicina, Medicina veterinaria
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