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Fig. 1

Fold changes of gene transcription in PBMCs from PTB+ and healthy animals upon PPDa stimulation. Fold change values from individual animals are represented by squares (PTB+ animals) and circles (healthy animals). Median and interquartile ranges are shown. Relative gene transcription was calculated with the 2-ΔΔCt method with E correction and using glyceraldehyde 3-phosphate dehydrogenase mRNA as the reference gene and the non-stimulated condition as the calibrator. Data were analysed using a two-tailed unpaired Mann-Whitney test CD14 – Cluster of differentiation 14; CXCL10 – C-X-C motif chemokine ligand 10; PTB – paratuberculosis; IFN-γ – interferon gamma; IL-1R – interleukin 1 receptor; IL-22 – interleukin 22; MMP9 – matrix metallopeptidase 9; THBS1 – thrombospondin 1; *** – P < 0.001
Fold changes of gene transcription in PBMCs from PTB+ and healthy animals upon PPDa stimulation. Fold change values from individual animals are represented by squares (PTB+ animals) and circles (healthy animals). Median and interquartile ranges are shown. Relative gene transcription was calculated with the 2-ΔΔCt method with E correction and using glyceraldehyde 3-phosphate dehydrogenase mRNA as the reference gene and the non-stimulated condition as the calibrator. Data were analysed using a two-tailed unpaired Mann-Whitney test CD14 – Cluster of differentiation 14; CXCL10 – C-X-C motif chemokine ligand 10; PTB – paratuberculosis; IFN-γ – interferon gamma; IL-1R – interleukin 1 receptor; IL-22 – interleukin 22; MMP9 – matrix metallopeptidase 9; THBS1 – thrombospondin 1; *** – P < 0.001

Results of ELISA, PCR test in faecal material and fold change transcription of target genes of animals used in this study

Animal ID ELISA PCR IFN-γ IL-22 CXCL10 THBS1 IL-1R MMP9 CD14
1 + + 3.68 4.27 6.10 −7.44 −2.28 −2.73 −1.25
2 + + −2.96 2.84 −3.20 −10.98 −4.09 −3.05 −3.35
3 + 1.31 6.34 5.56 −17.34 −1.31 −1.79 −2.97
4 + + 2.99 9.19 21.76 1.49 3.51 1.57 −1.89
5 + 1.74 7.52 1.65 −8.02 −1.39 1.40 −1.93
6 + + −1.11 4.63 −1.48 −1.96 1.40 2.20 1.21
7 + 4.27 288.74 33.97 −58.89 2.52 5.65 1.01
8 + 1.41 317.41 8.63 −44.74 1.97 3.26 −1.77
9 + −1.21 5.59 −2.17 −11.42 −2.48 −4.06 −3.15
10 + + −1.17 1.42 2.86 −5.07 1.16 1.25 1.11
11 + 9.00 1.93 26.86 −4.56 −1.36 3.07 1.20
12 + + −2.40 −219.17 8.20 −4.81 1.67 −3.02 1.11
13 + 17.13 389.80 11.52 −4.28 1.88 1.55 −2.38
14 2.54 8.43 25.62 −1.58 1.47 −1.01 −1.90
15 2.20 16.74 3.71 12.23 1.45 −1.54 1.22
16 1.44 22.23 1.05 −1.21 2.32 1.98 1.26
17 −2.44 −1.14 −1.19 −5.36 −1.03 −2.41 −2.91
18 −7.21 −2.51 −2.16 10.83 1.79 2.95 −1.09
19 −2.37 1.11 1.47 −1.65 3.72 1.70 −1.65
20 6.07 56.45 20.35 −2.53 −1.45 −2.05 −1.46
21 3.46 27.63 15.70 −1.65 −1.25 −1.60 −1.10
22 5.85 14.44 17.66 −1.56 −1.27 −2.25 −2.44
23 −2.94 ND −1.02 −2.35 5.60 1.55 −1.38
24 6.29 27.91 1.75 10.66 6.98 6.93 5.50
25 2.42 36.69 1.73 2.88 2.78 1.32 1.25
26 −1.45 3.81 −1.49 −1.07 −1.32 −1.64 −1.31
27 11.67 45.12 9.14 −3.9 −1.55 −2.84 −1.98
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Sujets de la revue:
Life Sciences, Molecular Biology, Microbiology and Virology, other, Medicine, Veterinary Medicine