<abstract xmlns="http://www.w3.org/1999/xhtml">

<sec id="j_jvetres-2023-0007_s_005"><h3>Introduction</h3>
<p><italic>Mycobacterium bovis</italic> and <italic>Mycobacterium avium</italic> subsp. <italic>paratuberculosis</italic>, respectively the causative agents of bovine tuberculosis (bTB) and bovine paratuberculosis (PTB), share a high number of antigenic proteins. This characteristics makes the differential diagnosis of the diseases difficult. The interferon gamma (IFN-γ), C-X-C motif chemokine ligand 10 (CXCL10), matrix metallopeptidase 9 (MMP9), interleukin 22 (IL-22) and thrombospondin 1 (THBS1) bovine genes have already been shown to be accurate transcriptional biomarkers of bTB. In order to improve the diagnosis of bTB and PTB, in the present study we evaluated the risk of false positivity of these bTB biomarkers in cattle with PTB.</p></sec>
<sec id="j_jvetres-2023-0007_s_006"><h3>Material and Methods</h3>
<p>The transcription of these genes was studied in 13 PTB-infected cattle, using <italic>Mycobacterium avium</italic> subsp. <italic>paratuberculosis</italic> (MAP)-stimulated peripheral blood mononuclear cells (PBMC).</p></sec>
<sec id="j_jvetres-2023-0007_s_007"><h3>Results</h3>
<p>Overall, the levels of IFN-γ, CXCL10, MMP9 and IL-22 transcripts in MAP-stimulated PBMC failed to differentiate animals with PTB from healthy animals. However, as bTB-afflicted cattle do, the MAP-infected group also displayed a lower level of THBS1 transcription than the non-infected animals.</p></sec>
<sec id="j_jvetres-2023-0007_s_008"><h3>Conclusion</h3>
<p>The results of this study add new specificity attributes to the levels of transcription of IFN-γ, CXCL10, MMP9 and IL-22 as biomarkers for bTB.</p>
</sec>
</abstract>

Introduction
Mycobacterium bovis and Mycobacterium avium subsp. paratuberculosis, respectively the causative agents of bovine tuberculosis (bTB) and bovine paratuberculosis (PTB), share a high number of antigenic proteins. This characteristics makes the differential diagnosis of the diseases difficult. The interferon gamma (IFN-γ), C-X-C motif chemokine ligand 10 (CXCL10), matrix metallopeptidase 9 (MMP9), interleukin 22 (IL-22) and thrombospondin 1 (THBS1) bovine genes have already been shown to be accurate transcriptional biomarkers of bTB. In order to improve the diagnosis of bTB and PTB, in the present study we evaluated the risk of false positivity of these bTB biomarkers in cattle with PTB.
Material and Methods
The transcription of these genes was studied in 13 PTB-infected cattle, using Mycobacterium avium subsp. paratuberculosis (MAP)-stimulated peripheral blood mononuclear cells (PBMC).
Results
Overall, the levels of IFN-γ, CXCL10, MMP9 and IL-22 transcripts in MAP-stimulated PBMC failed to differentiate animals with PTB from healthy animals. However, as bTB-afflicted cattle do, the MAP-infected group also displayed a lower level of THBS1 transcription than the non-infected animals.
Conclusion
The results of this study add new specificity attributes to the levels of transcription of IFN-γ, CXCL10, MMP9 and IL-22 as biomarkers for bTB.

Assessment of tuberculosis biomarkers in paratuberculosis-infected cattle

Journal of Veterinary Research

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

{"article-title":"Assessment of tuberculosis biomarkers in paratuberculosis-infected cattle"}

Introduction
Mycobacterium bovis and Mycobacterium avium subsp. paratuberculosis, respectively the causative agents of bovine tuberculosis (bTB) and bovine...

Assessment of tuberculosis biomarkers in paratuberculosis-infected cattle

Publié en ligne: 09 mars 2023

Pages: 55 - 60

Reçu: 27 juil. 2022

Accepté: 10 févr. 2023

DOI: https://doi.org/10.2478/jvetres-2023-0007

Mots clés
biomarkers, bovine tuberculosis, bovine paratuberculosis

© 2023 L.I. Klepp et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Assessment of tuberculosis biomarkers in paratuberculosis-infected cattle

Publié en ligne: 09 mars 2023

Pages: 55 - 60

Reçu: 27 juil. 2022

Accepté: 10 févr. 2023

DOI: https://doi.org/10.2478/jvetres-2023-0007

Mots clésbiomarkers, bovine tuberculosis, bovine paratuberculosis

© 2023 L.I. Klepp et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Mots clés
biomarkers, bovine tuberculosis, bovine paratuberculosis