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Effect of extender supplementation with low-molecular-weight antioxidants on selected quality parameters of cryopreserved canine spermatozoa

Marek Lecewicz

Marek Lecewicz

Department of Animal Biochemistry and Biotechnology, University of Warmia and MazuryOlsztyn, Poland
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Lecewicz, Marek
, 
Rafał Strzeżek

Rafał Strzeżek

Department of Animal Biochemistry and Biotechnology, University of Warmia and MazuryOlsztyn, Poland
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Strzeżek, Rafał
, 
Władysław Kordan

Władysław Kordan

Department of Animal Biochemistry and Biotechnology, University of Warmia and MazuryOlsztyn, Poland
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Kordan, Władysław
 et 
Anna Majewska

Anna Majewska

Department of Animal Biochemistry and Biotechnology, University of Warmia and MazuryOlsztyn, Poland
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Majewska, Anna
  
07 juil. 2018
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Publié en ligne: 07 juil. 2018
Pages: 221 - 227
Reçu: 03 janv. 2018
Accepté: 12 juin 2018
DOI: https://doi.org/10.2478/jvetres-2018-0032
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© 2018 Marek Lecewicz et al., published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Fig. 1

The effect of semen extender supplementation with different concentrations and combinations of Trolox (water-soluble vitamin E) and L-ascorbic acid (vitamin C) on the total motility of frozen-thawed canine spermatozoa during 2 h of incubation. The presented values are means ±SD for 20 ejaculates from four dogs. Values marked with different letters (a, b) differ significantly at P ≤ 0.05
The effect of semen extender supplementation with different concentrations and combinations of Trolox (water-soluble vitamin E) and L-ascorbic acid (vitamin C) on the total motility of frozen-thawed canine spermatozoa during 2 h of incubation. The presented values are means ±SD for 20 ejaculates from four dogs. Values marked with different letters (a, b) differ significantly at P ≤ 0.05

Fig. 2

The effect of semen extender supplementation with different concentrations of low-molecular-weight antioxidants on the progressive motility of frozen-thawed canine spermatozoa during 2 h of incubation. The presented values are means ±SD for 16 ejaculates from four dogs. Values marked with different letters (a, b) differ significantly at P ≤ 0.05
The effect of semen extender supplementation with different concentrations of low-molecular-weight antioxidants on the progressive motility of frozen-thawed canine spermatozoa during 2 h of incubation. The presented values are means ±SD for 16 ejaculates from four dogs. Values marked with different letters (a, b) differ significantly at P ≤ 0.05

Fig. 3

The effect of semen extender supplementation with different concentrations of low-molecular-weight antioxidants on plasma membrane integrity in frozen-thawed canine spermatozoa during 2 h of incubation. The presented values are means ±SD for 16 ejaculates from four dogs. Values marked with different letters (a, b) differ significantly at P ≤ 0.05
The effect of semen extender supplementation with different concentrations of low-molecular-weight antioxidants on plasma membrane integrity in frozen-thawed canine spermatozoa during 2 h of incubation. The presented values are means ±SD for 16 ejaculates from four dogs. Values marked with different letters (a, b) differ significantly at P ≤ 0.05

Fig. 4

The effect of semen extender supplementation with different concentrations of low-molecular-weight antioxidants on mitochondrial activity in frozen-thawed canine spermatozoa during 2 h of incubation. The presented values are means ±SD for 16 ejaculates from four dogs. Values marked with different letters (a, b) differ significantly at P ≤ 0.05
The effect of semen extender supplementation with different concentrations of low-molecular-weight antioxidants on mitochondrial activity in frozen-thawed canine spermatozoa during 2 h of incubation. The presented values are means ±SD for 16 ejaculates from four dogs. Values marked with different letters (a, b) differ significantly at P ≤ 0.05
Langue:
Anglais
Périodicité:
4 fois par an
Sujets de la revue:
Sciences de la vie, Biologie moléculaire, Microbiologie et virologie, Sciences de la vie, autres, Médecine, Médecine vétérinaire
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