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Fig. 1
The effect of semen extender supplementation with different concentrations and combinations of Trolox (water-soluble vitamin E) and L-ascorbic acid (vitamin C) on the total motility of frozen-thawed canine spermatozoa during 2 h of incubation. The presented values are means ±SD for 20 ejaculates from four dogs. Values marked with different letters (a, b) differ significantly at P ≤ 0.05
Fig. 2
The effect of semen extender supplementation with different concentrations of low-molecular-weight antioxidants on the progressive motility of frozen-thawed canine spermatozoa during 2 h of incubation. The presented values are means ±SD for 16 ejaculates from four dogs. Values marked with different letters (a, b) differ significantly at P ≤ 0.05
Fig. 3
The effect of semen extender supplementation with different concentrations of low-molecular-weight antioxidants on plasma membrane integrity in frozen-thawed canine spermatozoa during 2 h of incubation. The presented values are means ±SD for 16 ejaculates from four dogs. Values marked with different letters (a, b) differ significantly at P ≤ 0.05
Fig. 4
The effect of semen extender supplementation with different concentrations of low-molecular-weight antioxidants on mitochondrial activity in frozen-thawed canine spermatozoa during 2 h of incubation. The presented values are means ±SD for 16 ejaculates from four dogs. Values marked with different letters (a, b) differ significantly at P ≤ 0.05