Pratylenchus araucensis (Rhabditida: Pratylenchidae) a Widely Distributed Nematode in Musa spp. From Colombia

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Morphometric data of studied and reference populations of Pratylenchus araucensis_

Characteristic	Plantain, Zabaletas - Buenaventura	Plantain, Delfina - Buenaventura	Plantain, Caldas	Plantain, Calarcá - Quindío	Banana, Calarcá - Quíndio	Musa sp. Arauca, type population (Múnera et al., 2009)
	n = 19	n = 14	n = 19	n = 15	n = 10	n = 40
L	456 ± 24.0	501.8 ±56.0	484.5 ± 24.7	549.2 ± 13.9	551.3 ± 13.5	462 ± 31
	(401.2 - 496.3)	(449.3-668.7)	(444.4 - 538.0)	(518.4-578.3)	(534.7 - 570.4)	(376-511)
a	26.3 ±2.1	28.2 ± 1.9	29.9 ±2.0	25.2 ±1.3	24.8 ± 1.4	23.9 ±2.3
	(20 - 29.8)	(23.5-31)	(23.0-32.8)	(21.6-26.9)	(21.6-26.3)	(19.6-29.2)
b	5.8 ±1.0	6.1 ±0.7	6.4 ±0.5	6.2 ±1.7	6.7 ±1.4	6.2 ±0.6
	(4.7-7.0)	(5.1 -6.9)	(4.9 - 7.3)	(5.1 -7.4)	(5.5-7.7)	(4.8 - 7.8)
b'	4.4 ±0.6	4.7 ±0.2	4.3 ±0.5	4.1 ±0.8	4.5 ±1.0	4.7 ±0.4
	(4.0-5.7)	(3.7-6.0)	(3.7-5.2)	(3.7-5.0)	(4.0 - 4.9)	(3.6 - 5.9)
c	17.3 ±1.2	18.2 ± 1.5	18.9± 1.3	18.3 ±1.0	17.5 ±0.4	17.6 ±1.4
	(14.1 -22.3)	(14.9-21.8)	(15.2-23.4)	(16.7-20.2)	(16.6-18.0)	(14.5-21.1)
c'	2.3 ±0.5	2.5 ±0.4	2.6 ± .02	2.1 ±0.1	2.2 ±0.1	2.3 ±0.3
	(1.4-2.8)	(1.7-3.0)	(1.9-3.1)	(2.0-2.3)	(2.1 -2.5)	(1.7-2.9)
V%	76.7 ±1.8	77.6 ± 1.1	80.1 ± 1.6	76.5 ± 1.95	74.7 ±0.6	78 ± 1.2
	(73.9-80.1)	(75.7 - 79.4)	(76.5 - 83.6)	(72.9-81.4)	(74.0 - 75.9)	(75 - 80)
Stylet length	12.1 ±0.6	12.6± 1.1	11.9 ±0.6	14.4 ±0.4	14.7 ±0.4	15.3 ±0.4
	(11.1 -13.1)	(10.7-14.0)	(10.6-12.9)	(13.5-14.9)	(14.2-15.6)	(14.7-15.9)
Tail length	26.3 ±2.0	27.5 ± 1.8	25.7 ± 1.8	30.1 ±2.0	31.5 ±0.8	26.3 ±2.5
	(20.9-29.6)	(24.0-31.1)	(22.0-29.4)	(27.1 -34.6)	(30.2-32.7)	(21.5-31.9)
DGO	2.8 ±0.6	2.9 ±0.5	2.6 ±0.4	2.3 ±0.1	2.5 ±0.1	2.7 ±0.4
	(2.3-4.1)	(2.1 -3.6)	(2.0-3.2)	(2.1 -2.5)	(2.3-2.6)	(1.8-3.1)
Maximum body	16.7 ±1.5	17.8 ±2.0	15.7 ± 1.2	21.8 ±1.4	22.3 ± 1.3	17.0 ±1.8
diameter	(13.7-19.7)	(14.0-20.5)	(12.3-17.2)	(20.5-26.0)	(21.2-24.7)	(13.5-20.9)
Anal body	11.1 ±0.8	11.6 ±0.9	10.2 ± 0.8	14.1 ±0.6	14.1 ± 0.6	11.4 ± 1.5
diameter	(9.7-12.5)	(10.0-13.2)	(9.0 - 11.5)	(13.1 -15.2)	(13.2-14.8)	(8.6-17.2)
Number of head	2.0 ±0.0	2.0 ±0.0	2.0 ±0.0	2.0 ±0.0	2.0 ±0.0
annuli	(2.0-2.0)	(2.0-2.0)	(2.0-2.0)	(2.0-2.0)	(2.0-2.0)
Lip region width	6.1 ±0.5	7.1 ±0.9	6.3 ±0.5	8.2 ±0.4	8.1 ±0.3	7.2 ±0.3
	(4.7-6.8)	(5.6-9.1)	(5.5 - 7.3)	(7.7-8.9)	(7.5-8.5)	(6.7 - 7.4)
Lip region height	2.5 ±0.6	2.8 ±0.3	2.5 ±0.4	2.1 ±0.2	2.4 ±0.2	2.5 ±0.7
	(1.5-3.6)	(2.3-3.2)	(1.8-3.1)	(1.8-2.4)	(1.9-2.6)	(2.0-4.0)
Pharyngeal gland	35.7 ±5.3	31.7 ± 1.4	32.8 ±4.7	28.6 ±3.6	40.5 ± 1.1	24.2 ± 7.7
length	(25.8-45.7)	(30.1 -34.4)	(24.0-39.2)	(24.1 -33.8)	(38.9-42.2)	(10.4-46.6)
PUS	18.5 ±5.5	31.2 ± 1.6	24.5 ±4.8	29.6 ±0.8	30.4 ± 1.2	18.1 ±4.0
	(8.4-27.3)	(29.8-33.2)	(14.8-32.0)	(28.6 - 31.4)	(28.8-32.0)	(9.8-24.5)

Correlations between the first three main components and the morphometric parameters of P_ araucensis females_

Variable	Main component
	Prin1	Prin2	Prin3
Stylet length (μm)	0.298	0.440	-0.159
Vulvar position (%)	-0.288	0.431	0.178
Post-uterine sac length (μm)	0.315	0.176	0.407
Overall length	0.330	0.370	0.245
Tail length (μm)	0.379	-0.046	0.235
Maximum body width (μm)	0.396	0.005	-0.182
Width body anus (μm)	0.377	0.133	-0.188
Head region width (μm)	0.242	-0.345	0.447
Index a (μm)	-0.294	0.122	0.620
Index c (μm)	-0.182	0.547	-0.097

Information of D2–D3 rDNA and COI mtDNA regions downloaded from GenBank and obtained in the present study_

Species name	Location	D2–D3 (LSU) accession number	COI accession number	Reference or source
Pratylenchus araucensis	Colombia	MZ636668; MZ636669 MZ636670; MZ636671 MZ636672; MZ636673 MZ636674; MZ636675 MZ636676; MZ636677 MZ636678; MZ636679 MZ636680; MZ636681 MZ636682; MZ636683	MZ636684; MZ636685 MZ636686; MZ636687 MZ636688; MZ636689 MZ636690; MZ636691 MZ636692; MZ636693 MZ636694; MZ636695 MZ636696; MZ636697 MZ636698; MZ636699 MZ636700	Present study
P. araucensis	Colombia	FJ463262; FJ463261; FJ463260; FJ463275	-	Múnera et al. (2009)
P. hippeastri	China	KR029084	KY424099	Wang et al. (2016)
P. hippeastri	China	KP161608	KY424098	Unpublished
P. hippeastri	South Africa	MH324472	-	Shokoohi (2018)
P. hippeastri	Japan	KC796706; KC796707	-	Wujian et al. (2014)
P. scribneri	USA	KX842632	-	Huang and Yan (2017)
P. scribneri	China	MK209593	-	Li et al. (2018)
P. scribneri	China	-	KY424093	Unpublished
P. scribneri	China	-	KX349425	Liu et al. (2016)
P. pseudocoffeae	Korea	KT175532; KT175533	-	Kim et al. (2016)
P. pseudocoffeae	Costa Rica	KT971360	-	Zamora et at. (2016)
P. pseudocoffeae	China	-	KY424089	Unpublished
P. loosi	China	KF430796; KF430797	-	Unpublished
P. loosi	Italy	-	LR215647; LR215648	Unpublished
P. coffeae	China	MN592778; MN227243; MN588282		Li et al. (2020)
P. coffeae	China	-	KX349421	Liu et al. (2016)
P. coffeae	China	-	MN366418	Unpublished
P. coffeae	USA	-	KU198943; KU198942	Troccoli et al. (2016)
P. speijeri	China	KF974701; KF974698; KF974697		Unpublished
P. speijeri	China		KY424088; KY424087	Unpublished
P. penetrans	USA	KY969632; KY969631		Baidoo et al. (2017)
P. penetrans	Korea	-	MN746802; MN746803; MN746801	Mwamula et al. (2020)
P. kumamotoensis	Korea	KT175529; KT175530; KT175525	-	Kim et al. (2016)
P. parazeae	China	KP903444; KP903445; KP903443; KP903442		Wang et al. (2015)
P. parazeae	China		KY424116; KX349424; KY424115	Unpublished
P. zeae	Brazil	MW363009; MW363001	-	Abade (2020)
P. zeae	China		KX349414; KX349416; KX349419	Liu et al. (2016)
P. horti	Belgium	-	MK114154; MK114153; MK114152	Nguyen et al. (2018)
P. brachyurus	China	-	KX349420; KY424082; KY424083; KY424080	Unpublished