<abstract xmlns="http://www.w3.org/1999/xhtml">

<p>Pistachio yield is annually reduced due to root-knot nematode infections. In order to evaluate its resistance to <italic>Meloidogyne javanica</italic>, three domestic pistachio rootstocks, namely, Badami, Ghazvini and Sarakhs, and a wild pistachio, Baneh (<italic>Pistacia atlantica</italic> subsp. <italic>mutica</italic>), were selected. Their response to the nematode infection was evaluated based on different plant and nematode indexes, 120 days post-inoculation (dpi). The penetration and development rate of nematode in roots of these four pistachio rootstocks were evaluated at different time points by acid fuchsin staining. Based on the measured indexes, Badami, Ghazvini, Sarakhs, and Baneh rootstocks ranked as susceptible, moderately resistant, moderately resistant, and resistant, respectively. The penetration rate of second-stage nematode juveniles (J2) into four rootstocks was discussed. The first “midstage” or swollen juveniles appeared at 4 dpi but to a lesser extent in Ghazvini, Sarakhs, and Baneh cultivars. The first females were seen in Badami at 21 dpi, in Ghazvini and Sarakhs at 35 dpi, and in Baneh at 45 dpi. Three types of defense responses were distinguished and characterized in the examined pistachio rootstocks: (i) a hypersensitive response (HR)-like reaction in the cortex in Ghazvini, Sarakhs, and Baneh root tips at 4 dpi and 6 dpi; (ii) an HR response, degrading J2 which induce giant cells in the vascular cylinder of all rootstocks, at 6 dpi and 10 dpi; and (iii) an HR response, degrading females and giant cells in the vascular cylinder of all rootstocks at 15 dpi onward. These observations open new fields of study in breeding programs of this crop.</p></abstract>

Pistachio yield is annually reduced due to root-knot nematode infections. In order to evaluate its resistance to Meloidogyne javanica, three domestic pistachio rootstocks, namely, Badami, Ghazvini and Sarakhs, and a wild pistachio, Baneh (Pistacia atlantica subsp. mutica), were selected. Their response to the nematode infection was evaluated based on different plant and nematode indexes, 120 days post-inoculation (dpi). The penetration and development rate of nematode in roots of these four pistachio rootstocks were evaluated at different time points by acid fuchsin staining. Based on the measured indexes, Badami, Ghazvini, Sarakhs, and Baneh rootstocks ranked as susceptible, moderately resistant, moderately resistant, and resistant, respectively. The penetration rate of second-stage nematode juveniles (J2) into four rootstocks was discussed. The first “midstage” or swollen juveniles appeared at 4 dpi but to a lesser extent in Ghazvini, Sarakhs, and Baneh cultivars. The first females were seen in Badami at 21 dpi, in Ghazvini and Sarakhs at 35 dpi, and in Baneh at 45 dpi. Three types of defense responses were distinguished and characterized in the examined pistachio rootstocks: (i) a hypersensitive response (HR)-like reaction in the cortex in Ghazvini, Sarakhs, and Baneh root tips at 4 dpi and 6 dpi; (ii) an HR response, degrading J2 which induce giant cells in the vascular cylinder of all rootstocks, at 6 dpi and 10 dpi; and (iii) an HR response, degrading females and giant cells in the vascular cylinder of all rootstocks at 15 dpi onward. These observations open new fields of study in breeding programs of this crop.

Penetration and Development of <italic>Meloidogyne javanica</italic> on Four Pistachio Rootstocks and Their Defense Responses

Journal of Nematology

This work is licensed under the Creative Commons Attribution 4.0 International License.

Penetration and Development of  on Four Pistachio Rootstocks and Their Defense Responses

Pistachio yield is annually reduced due to root-knot nematode infections. In order to evaluate its resistance to Meloidogyne javanica, three domestic pistachio...

Penetration and Development of Meloidogyne javanica on Four Pistachio Rootstocks and Their Defense Responses

Article Category: Research Paper

Publié en ligne: 20 janv. 2023

Pages: -

Reçu: 08 juin 2022

DOI: https://doi.org/10.2478/jofnem-2022-0056

Mots clés
development, hypersensitive response, penetration, pistachio, resistance, root-knot nematode

© 2022 Walter de Gruyter GmbH, Berlin/Boston

This work is licensed under the Creative Commons Attribution 4.0 International License.

Penetration and Development of Meloidogyne javanica on Four Pistachio Rootstocks and Their Defense Responses

Article Category: Research Paper

Publié en ligne: 20 janv. 2023

Pages: -

Reçu: 08 juin 2022

DOI: https://doi.org/10.2478/jofnem-2022-0056

Mots clésdevelopment, hypersensitive response, penetration, pistachio, resistance, root-knot nematode

© 2022 Walter de Gruyter GmbH, Berlin/Boston

This work is licensed under the Creative Commons Attribution 4.0 International License.

Mots clés
development, hypersensitive response, penetration, pistachio, resistance, root-knot nematode