Evaluating Efficacy of Fumagilin-B® Against Nosemosis and Tracking Seasonal Trends of Nosema spp. in Nova Scotia Honey Bee Colonies
, , et
18 nov. 2020
À propos de cet article
Catégorie d'article: Original Article
Publié en ligne: 18 nov. 2020
Pages: 277 - 286
Reçu: 28 janv. 2020
Accepté: 03 oct. 2020
DOI: https://doi.org/10.2478/jas-2020-0025
Mots clés
© 2020 Robyn McCallum et al., published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.
Fig. 1

Honey bee sampling schedule and description from February 2018 to March 2019 in Nova Scotia colonies
Date | Description |
---|---|
28 February 2018 | First sample collection |
27 March 2018 | Regular monthly sample collection |
23 April 2018 | Pre spring treatment |
24 & 25 April 2018 | Spring treatment and feeding (no samples collected) |
27 April 2018 | 2–3 days post spring treatment |
01 May 2018 | 5–6 days post spring treatment |
07 May 2018 | 12–13 days post spring treatment |
10 May 2018 | 15–16 days post spring treatment |
29 May 2018 | 34–35 days post spring treatment/Regular monthly sample collection |
26 June 2018 | Regular monthly sample collection |
26 July 2018 | Regular monthly sample collection |
29 August 2018 | Regular monthly sample collection |
03 October 2018 | Pre fall treatment |
05 & 06 October 2018 | Fall treatment and feeding (no samples collected) |
10 October 2018 | 4–5 days post fall treatment |
16 October 2018 | 10–11 days post fall treatment |
19 October 2018 | 13–14 days post fall treatment |
29 October 2018 | 23–24 days post fall treatment |
14 March 2019 | Final sample collection |
List of primers used to identify Nosema species
Target | Primer | Sequence (5′–3′) | Amplicon size (bp) |
---|---|---|---|
RPS5 | RPS5-F | AATTATTTGGTCGCTGGAATTG | 105 |
RPS5-R | TAACGTCCAGCAGAATGTGGTA | ||
RNA polymerase | Ncpol-F | TGG GTT CCC TAA ACC TGG TGG TTT | 662 |
Ncpol-R | TCA CAT GAC CTG GTG CTC CTT CT | 297 | |
Napol-F | AGC AAG AGA CGT TTC TGG TAC CTC A | ||
Napol-R | CCT TCA CGA CCA CCC ATG GCA |