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In vitro plant regeneration of Christmas cactus (Schlumbergera truncata (Haw.) Moran) by indirect morphogenesis

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13 mars 2021
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Figure 1

Plant regeneration Schlumbergera truncata (Haw.) Moran via indirect morphogenesis in vitro: A – aseptic viable culture and initiation formation of new cladode; B – morphogenic callus tissue on MS with 1.0 mg/l BA and 0.5 mg/l NAA; C – friable callus culture; D, E – root system of plants at 1.0 mg/l IBA; F – rooted plant culture on modified MS
Plant regeneration Schlumbergera truncata (Haw.) Moran via indirect morphogenesis in vitro: A – aseptic viable culture and initiation formation of new cladode; B – morphogenic callus tissue on MS with 1.0 mg/l BA and 0.5 mg/l NAA; C – friable callus culture; D, E – root system of plants at 1.0 mg/l IBA; F – rooted plant culture on modified MS

The effect of growth regulators and conditions of illumination on the induction of callus formation in S_ truncata plant explants, 25 days in in vitro culture

Variants The composition of the nutrient medium Conditions of illumination
Light room (2.0−3.0 klx) callus formation frequency (mean ± standard error), % Thermostat (without light) callus formation frequency (mean ± standard error), %
C Control 0 0
1 MS + 2.0 mg/l 2.4-D 0 33.3±8.8
2 MS + 1.0 mg/l BA + 0.5 mg/l NAA 90.0±5.8 93.3±3.3

Growth characteristics of S_ truncata plant shoots cultivated on modified nutrient media MS, 60 days in vitro culture

Variants The composition of the nutrient medium The length of one segment section of sprouts (mean ± standard error), cm Plants multiplication factor (mean ± standard error) Pigmentation The presence of the root system
C hormones free 1.0±0.2 2.0±0.3 light green +
1 2.0 mg/l BA 0.5±0.1 10.0±0.7 light green -
2 0.5 mg/l kinetin BA + 0.5 mg/l 1.2±0.2 8.8±0.6 green +
3 1.0 mg/l IBA 1.1±0.2 1.6±0.2 green +
4 0.25 mg/l kinetin 1.4±0.1 3.8±0.4 green +
5 activated 0.25 mg/carbon l kinetin adsorbent + 2 g/l 1.5±0.1 4.2±0.4 green +
Langue:
Anglais
Périodicité:
4 fois par an
Sujets de la revue:
Sciences de la vie, Sciences végétales, Médecine, Médecine vétérinaire