<abstract xmlns="http://www.w3.org/1999/xhtml">

<p>Plants of <italic>Schlumbergera truncata</italic> (Haw.) Moran were obtained by indirect morphogenesis from the segment section of shoots in vitro, they were multiplied and rooted. Also were determined the effect of the lighting regime, the composition of the nutrient medium on the consistency and frequency of callus formation. The studies were conducted during 2016–2018. The mode of effective sterilization (more than 90%) of <italic>S. truncata</italic> plant explants using 0.1% HgCl<sub>2</sub> for 7–8 min was established. Optimal conditions for the induction of callus formation in stem node segments of <italic>S. truncata</italic> plants (rate more than 90% and significant growth) were created on MS (<xref ref-type="bibr" rid="j_ffp-2021-0007_ref_011">Murashige and Skoog 1962</xref>) nutrient medium supplemented with 1.0 mg/l BAP (6-benzylaminopurine) and 0.3 mg/l NAA (1-naphthylacetic acid) under conditions of placement on the nutrient medium and doing a significant number of cuts on the explants. The light intensity of 2.0–3.0 klx, obtained by a callus of dense consistency of dark green pigmentation, when using the thermostat condition without illumination, the callus had loose consistency, dark yellow pigmentation. It is established that the influence of the lighting regime and the composition of the nutrient medium on the frequency of callus formation is statistically significant. The largest number of shoots was obtained on the MS medium with the addition of 2.0 mg/l of BA. At the same times, shoot proliferation and root induction in such numbers were observed on MS culture medium with the addition of 0.5 mg/l BA and 0.5 mg/l kinetin (multiplication factor – 8.8±0.6 per 60-day cultivation cycle).</p></abstract>

Plants of Schlumbergera truncata (Haw.) Moran were obtained by indirect morphogenesis from the segment section of shoots in vitro, they were multiplied and rooted. Also were determined the effect of the lighting regime, the composition of the nutrient medium on the consistency and frequency of callus formation. The studies were conducted during 2016–2018. The mode of effective sterilization (more than 90%) of S. truncata plant explants using 0.1% HgCl2 for 7–8 min was established. Optimal conditions for the induction of callus formation in stem node segments of S. truncata plants (rate more than 90% and significant growth) were created on MS (Murashige and Skoog 1962) nutrient medium supplemented with 1.0 mg/l BAP (6-benzylaminopurine) and 0.3 mg/l NAA (1-naphthylacetic acid) under conditions of placement on the nutrient medium and doing a significant number of cuts on the explants. The light intensity of 2.0–3.0 klx, obtained by a callus of dense consistency of dark green pigmentation, when using the thermostat condition without illumination, the callus had loose consistency, dark yellow pigmentation. It is established that the influence of the lighting regime and the composition of the nutrient medium on the frequency of callus formation is statistically significant. The largest number of shoots was obtained on the MS medium with the addition of 2.0 mg/l of BA. At the same times, shoot proliferation and root induction in such numbers were observed on MS culture medium with the addition of 0.5 mg/l BA and 0.5 mg/l kinetin (multiplication factor – 8.8±0.6 per 60-day cultivation cycle).

In vitro plant regeneration of Christmas cactus (<italic>Schlumbergera truncata</italic> (Haw.) Moran) by indirect morphogenesis

Folia Forestalia Polonica

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

In vitro plant regeneration of Christmas cactus ( (Haw.) Moran) by indirect morphogenesis

Plants of Schlumbergera truncata (Haw.) Moran were obtained by indirect morphogenesis from the segment section of shoots in vitro, they were multiplied and...

Variants	The composition of the nutrient medium	Conditions of illumination
Variants	The composition of the nutrient medium	Light room (2.0−3.0 klx) callus formation frequency (mean ± standard error), %	Thermostat (without light) callus formation frequency (mean ± standard error), %
C	Control	0	0
1	MS + 2.0 mg/l 2.4-D	0	33.3±8.8
2	MS + 1.0 mg/l BA + 0.5 mg/l NAA	90.0±5.8	93.3±3.3

Variants	The composition of the nutrient medium	The length of one segment section of sprouts (mean ± standard error), cm	Plants multiplication factor (mean ± standard error)	Pigmentation	The presence of the root system
C	hormones free	1.0±0.2	2.0±0.3	light green	+
1	2.0 mg/l BA	0.5±0.1	10.0±0.7	light green -	−
2	0.5 mg/l kinetin BA + 0.5 mg/l	1.2±0.2	8.8±0.6	green	+
3	1.0 mg/l IBA	1.1±0.2	1.6±0.2	green	+
4	0.25 mg/l kinetin	1.4±0.1	3.8±0.4	green	+
5	activated 0.25 mg/carbon l kinetin adsorbent + 2 g/l	1.5±0.1	4.2±0.4	green	+

In vitro plant regeneration of Christmas cactus (Schlumbergera truncata (Haw.) Moran) by indirect morphogenesis

Article Category: Short communication

Publicado en línea: 13 mar 2021

Páginas: 68 - 73

Recibido: 06 sept 2020

Aceptado: 25 nov 2020

DOI: https://doi.org/10.2478/ffp-2021-0007

© 2021 Oksana Chornobrov, Svitlana Bilous, published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Figure 1

The effect of growth regulators and conditions of illumination on the induction of callus formation in S. truncata plant explants, 25 days in in vitro culture

Growth characteristics of S. truncata plant shoots cultivated on modified nutrient media MS, 60 days in vitro culture